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We narrowed to 653 results for: EPO

Showing: 331 - 340 of 653 results

  2. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...piggyBac plasmids    Tol2  Tol2 was the first reported active DNA transposon in vertebrates. It was discovered...mutations. In these screens, the transposons encode reporter genes, mutagenic cassettes, or barcodes. When ...

  3. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... self-labeling tools: Tetracysteine: The first reported chemical labeling tag, from Roger Tsien’s lab ...collection. CLIP-tag: Also in 2008, Kai Johnsson’s lab reported another AGT-based tag named CLIP-tag, which reacts...

  4. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... unbiased methods, high background BLESS Not reported Cell-based (in vitro) Can be used on tissue from...senstive to time since cell fixing HTGTS Not reported Cell-based Identifies translocations Limited by...

  6. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Plasmid 68285 SepOTSδ Plasmid 68286 SepOTSϵ Plasmid 68287 SepOTSζ Plasmid 68288 SepOTSη Plasmid 68289 SepOTSθ...68290 SepOTSι Plasmid 68291 SepOTSκ Plasmid 52054 SepOTSµ ( aka. B40 OTS) Plasmid 68294 SepOTSν Plasmid...Plasmid 34624 SepOTSα tRNA-Sep ( aka. pCAT112TAG-SepT) Plasmid 68283 SepOTSβ Plasmid 68284 SepOTSγ Plasmid ....Δserb.Amp S Plasmid 68292 SepOTSλ Plasmid 68307 SupD Plasmid 34623 SepOTSα SepRS/EF-Sep ( aka. pKD-SepRS-EFSep... Depositor Collections Rinehart Lab Phosphoprotein Reagents...188537 ) a better choice in place of the popular SepOTSλ ( 68292 ). An important technical note: pSerOTS-C1...can be generated with either phosphoserine using SepOTSλ ( 68292 ) or serine using supD tRNA ( 68307 ) at...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process by a novel reporter protein... DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters via electrochromic...genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes A turquoise...Calcium Ratiometric calcium biosensors from nested reporters of green- and orange-emitting proteins Ratiometric...imaging (Perceval) A genetically encoded fluorescent reporter of ATP:ADP ratio. Nat Methods. 2009 Feb . 6(2)...

  8. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...plasmids, which contain various promoters and reporters, and subsequently inserted into a Cas9-containing...-in-one CRISPR/Cas9 vector system.” Scientific Reports 4 (2014): 5400. PubMed PMID: 24954249. PubMed Central...

  9. Bacterial Expression Systems

    Type
    Collection
    ...Tagging Purification Controlled Expression Reporter Plasmids Reporter Plasmids Tagging and Visualization Purification...expression at a specific level. Return to Top Reporter Plasmids Reporter plasmids can be used to detect events...they activate expression of the reporter gene. Plasmid ID Promoter Reporter PI Purpose pRU Series Various...Purification Controlled Expression Reporter Plasmids You may also like… Plasmids 101 Addgene's Molecular...promoters, their associated transcription factors, and reporter genes. pCS-PesaRlux 47640 PesaR 3OC6HSL (Quorum...bacterium. Plasmids containing easily measurable reporter genes (eg. LacZ or Fluorescent Proteins ) under...transcription factor to bind to a small molecule, the reporter plasmid can be used to detect the presence of ...

  10. Luciferase Plasmid Collection

    Type
    Collection
    ...Multiple Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, ...transcriptional regulation and bioluminescent reporters. Plasmid...Highlights Empty Backbones Expression Constructs Reporter Constructs Luciferase is the enzyme responsible... common choice in assays ranging from use as a reporter gene in vitro and in vivo to use in biosensors...-lives, making them useful as transcriptional reporters. However, they are not secreted, so cells must...CMV-LUC2CP/intron/ARE, CMV-LUC2CP/ARE : A splicing reporter that contains an intron in the middle of the Firefly.... pKC-4.04, pKC-4.06 : Nonsense-mediated decay reporter and control plasmid utilizing Firefly luciferase...
Showing: 331 - 340 of 653 results