...project management. Mentorship is another invaluable resource: connecting with mentors who have made similar...management often means stepping into unfamiliar territory. Unlike solving technical problems in the lab,...lab, management involves a different skill set: mentoring, setting strategic goals, and fostering team success...for me. I began exploring alternative career trajectories within the industry, but the transition wasn... role is also crucial. I owe quite a bit to my mentors, as they encouraged me to explore small-scale opportunities...company by taking on responsibilities such as mentoring a junior colleague. Guiding an intern, in particular...and leveraging tools like the Myers-Briggs Type Indicator or DISC assessments can help you navigate team...

... your gene of interest in a plasmid and get the vector incorporated into the germline. To begin this process...knockdown. Addgene also has a number of insect vectors to consider. An easy way to see what is available...Find Gal4 and UAS plasmids at Addgene Choosing a vector to generate a transgenic fly There are a few important...important points to consider when choosing the vector you’d like to use to generate your new fly line:... gene to be incorporated into the genome? Some vectors incorporate into the genome randomly, while others...would you like to select for your flies? Sure, the vector will have an antibiotic resistance gene to select...transgene. One such marker is the white gene, which restores eye color to fly lines where the endogenous white...

...bacteria that have taken up this vector, but it won’t tell you whether the vector contains the gRNA insert or...X-gal allows you to identify cells that took up the vector and distinguish between plasmids that contain the...functional 𝛽-galactosidase. Blue-white screens rely on vectors where the gene of interest is inserted into the..., you will see two bands: one representing your vector backbone and the other representing the insert....doesn’t contain the insert, you will just see the vector backbone band. Or, you can choose an enzyme that...specific to your insert or by using primers that are vector specific but amplify a potential insert. If you...plasmid does not contain the insert. If you’re using vector-specific primers, look for size differences. You...

...feels that storytelling is an impactful tool for science communicators. “The power of a story is to make... to learn things quickly. Story telling. The ability to know what’s a story and how to hook your readers...there’s always plenty of writing: plain-language stories about Fred Hutch research, pieces for donors such...that can be a very painful process. And certain stories can make you feel like ‘I’m a terrible writer. ...through connections and that’s something that good storytellers in science can do.” Susan’s number one piece...social media as a scientist here Learn more about storytelling in science ...

...AMA I am a strong advocate for peer mentoring, but volunteer mentors are only able to go so far with their...coach do that is different from an adviser, boss or mentor? First, professional coaches have experience and...infrastructure are not always as effective in mentoring for this transition. Second, a coach will help...experiences. Good coaches will have loads of success stories and plenty of people to vouch for them. Trust...help you. A good coach usually will be a clear communicator and make direct suggestions for improvement ...online content for PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs...has an outstanding blog and, while her PhD is in History, I think she would be a good match as a coach for...

...scientists who were potential Addgene requestors and depositors. China is in one of the top 10 countries...using bacterial phage vectors with CRISPR/cas9 sensitizing gene cassettes. Thse vectors are engineered to ...world. With three Nobel Prize winners, one NIH Director and a co-founder of BGI-China presenting, it’s... discoveries and moving beyond DNA Eric Green, Director of the National Human Genome Research Institute...human tRNA genes and, until now, we've overlooked regulatory fragments of these transcripts. I remember when...was kicked off by Nobel Laureate (and Addgene depositor) Craig Mello. Taking his groundbreaking work in...Medicine presented another unexpected finding in the regulatory RNA field. The Rajewsky lab is studying circular...

...utility of the pRDA_526 vector. Moreover, the screen identified putative novel regulators of CD8+ T cell responses... here! Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School ...in immune cells have identified many putative regulators of immunity. However, in vitro screens cannot... of this system is a lentiviral gRNA expression vector (pRDA_526) that (1) enables efficient knockout ...model in vivo. In this screen, we used the pRDA_526 vector to knock out 30 genes putatively involved in CD8...responses to cancer. The screen recovered known regulators, such as Ptpn2, Pdcd1, and Zc3h12a, demonstrating...

...Administration of substances to laboratory animals: routes of administration and factors to consider. PubMed, 50...type of injection to use depends on a number of factors. You have to weigh the pros and cons of various...be injected, including cells, drugs, DNA, viral vectors, and viruses. All researchers must go through ...choice for administering different drugs and viral vectors. Like IV injections, intraperitoneal injections...common use of intradermal injections is studying inflammatory responses, as this method is useful for analyzing...for the delivery of various therapies and viral vectors. This injection sounds very disruptive, but surprisingly... This is most often used for delivery of viral vectors, though drug treatments are also possible. One ...

...into wide use, there were a variety of ways to monitor cell, organelle, and protein localization. For ...the FP timers, add an entire new dimension to monitoring localization; using FP timers, researchers can...Tsuboi et al.), limiting its use. Nonetheless, its creators noticed a few properties that have now become ...benefit of the common FP timers is that you can monitor age by expressing a single FP-timer protein or ... These separate proteins should be useful for monitoring cellular events occurring at varied time scales...fluorescent protein timers FP timers have been used to: Monitor dynamics of vesicle fusion and release from the...vesicle cargoes to FP timer mk-GO (Tsuboi et al.) Monitor gene expression in the developing pancreas by placing...

...bacteria, but have no fear - plasmids from Addgene depositors are making it easier than ever to use CRISPR  ...double stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage of this...activation of transcription including transcription factors, phage proteins, and RNAP components (Dong et al... al., 2018). They found a transcription factor called SoxS for CRISPRa. Adding complexity, they found ...been observed with Phagemids (Selle et al., 2020, Citorik et al., 2015, Bikard et al., 2014). When the CRISPR... on the presence of specific target sequences (Citorik et al., 2014). These could be genes, specific polymorphisms...refined by the addition of a uracil DNA glycosylase inhibitor and degradation tag on the fusion protein to limit...