We narrowed to 997 results for: lars;

...coding the molecular biology of the worm into a computer model that recapitulates how molecular biology ...get involved a in variety of aspects from the molecular biology to learning how to grow and care for the...but we need to develop the manual skills at the regular microscope, and the scripting skills at the automated...locomotion. Our most recent models of C. elegans molecular biology are based on the standard worm genome....Center (CGC) at the University of Minnesota has declared our makerspace an educational institution meaning...Silicon Valley as a student and then instructor at Singularity University at NASA Ames, and an instructor at...

...250 kDa Similar in size 8% 20–250 kDa Similar in size 10% 10–100 kDa Similar in size ...Conversely, if your protein of interest is large, look for a larger pore size (0.45 µm).  Types of transfers...important part of your western is your antibodies, particularly your primary antibodies. We always recommend...When choosing your lysis buffer, consider the subcellular location of your protein. Proteins located in...to overnight to run, and is recommended for very large proteins. A semi-dry transfer is faster — 3–30 minutes...differing amounts of available epitopes for that particular antibody, this truly does provide a relative,...

...noise, due to the large amount of dsDNA in most starting materials, and is not particularly sensitive. The...Protocol: How to Design a primer  Molecular Biology Reference Molecular Cloning Techniques References Bell... copy number assays for years. qPCR works like regular PCR, just with signaling molecules added to the...be affected by DNA quality and primer design, particularly for amplicons under 100 bp (Ma, 2015.) A schematic...ll have to consider:  Resources needed Most molecular biology labs and departments will have qPCR machines...

...columns had similar DNA yields. Additionally, columns regenerated up to five times had similar DNA yields...Addgene.org Find protocols for molecular biology and cloning Read our Molecular Biology Reference Visit our...plasmid miniprep protocol from Addgene follows a similar workflow as a column-based plasmid extraction kit... This method provides DNA yield and quality similar to that obtained with commercial kits, but in less...cellulose-based method was faster, cheaper, and had a similar level of sensitivity. Figure 2: Steps to ...

...fluorophores conjugated to molecular species with high affinity for endogenous molecular targets such as paclitaxel...conjugate, and the complexity of the system. A particularly desirable property for small-molecule labeling...removing excess dye from the sample, which is particularly important for samples where washing is difficult...and fluorogenic ligands. New fluorophores As a corollary to these innovative labeling strategies, several... these fluorophores are often bulky and contain polar groups (Figure 2b), which can preclude their use... and structure at the single-molecule level." Molecular cell 58.4 (2015): 644-659. PubMed PMID: 26000849...

...Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally...the insert site. A positive clone will produce a larger size product then a negative clone without the ...determine their size. Make sure to use an appropriate molecular weight standard for reference and to add a loading...-) will not. Backbone-specific primers (2) give larger sized products for positive clones (+) compared..., colony PCR is a useful tool to have in your molecular biology tool kit. Consider giving it a try next...Tips and tricks from the bench Don't pick too large of a colony. Too many bacteria can inhibit your ...Resources on Addgene.org Read about Choosing a Molecular Cloning Technique Learn to Perform a Bacterial...

... looking for some additional information or clarification on these vectors. Read on to find the answers...allow researchers to target transduction to a particular cell type or tissues; this is useful in gene ...therapy applications where a distinct subset of the cellular population needs to be targeted. Q6: Are transfer...self-inactivating, which is achieved by deleting a large portion of the 3'LTR in the transfer plasmid. The...not only the transgene but also that of adjacent cellular genes. Q7: Which genes are deleted or modified...through insertional mutagenesis. These risks are largely based upon the vector system used and the transgene...Library Resources on Addgene.org Addgene's most popular lentiviral plasmids Overview of Lentiviral Packaging...

...toolbox. However, both of these proteins are quite large — comprising approximately 1000–1400 amino acids...challenge for efficient delivery into cells, particularly via viral vectors. While viral delivery is a... cell types, it has a limited capacity to carry large DNA cargos. As a result, the search for smaller,... Cas12a, uses just one to cleave both strands. Similar to how Cas proteins require a PAM (protospacer ...capacity, which makes it difficult to accommodate the large coding sequence of commonly used nucleases like ..., Nasti, R. A., Vollbrecht, M., Starker, C. G., Clark, M. D., & Voytas, D. F. (2019). Plant gene editing...an Old Favorite A History of Genome Editing in Popular Culture Additional Resources on addgene.org Genome...

...A similar genetic code is used by most organisms on Earth, but different organisms have different preferences...reasons include: Metabolic pressures - it takes cellular resources to produce tRNAs that recognize different...contains a gene that’s been codon optimized for a particular organism, this will sometimes (but not always... purification procedures. Even if you produce a large amount of protein in your expression host of choice...cotranslational folding." Nature structural & molecular biology20.2 (2013): 237. PubMed PMID: 23262490...bias as a means to fine-tune gene expression." Molecular cell 59.2 (2015): 149-161. PubMed PMID: 26186290...featured topic page Resources on Addgene.org Molecular biology reference pages Genetic code E. coli strains...

...regions. PHP.eB, an enhanced version of PHP.B, shows similar transduction efficiency, but at a log lower dose...insertions and deletions (indels) most likely to have large phenotypic effects such as those that affected gene...pattern in the mouse brain.  Both labs then took similar routes to verify LY6A’s role as a receptor for ...transduce cells. The Wilson Lab generated results similar to the Deverman Lab when they looked at LY6A protein... B Transport across the Blood-Brain Barrier." Molecular Therapy 27.5 (2019): 912-921. PubMed PMID: 30819613... of AAV-PHP. B are limited to C57BL/6J mice." Molecular Therapy 26.3 (2018): 664-668. PubMed PMID: 29428298... across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP. B capsids." bioRxiv (2019...