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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 993 results for: Ran

Showing: 661 - 680 of 993 results

  1. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ... °C but preferentially nicks the non-target DNA strand. The lab then found gain-of-function mutations ...mutations that allows it to be repurposed for double strand breaks at target sites. Read the paper in Nature... of stable genomic integration and is easily transferred via conjugation.   Read the paper in Nature...

  2. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...ability to direct enzymes – from nucleases to transcription factors – to specific sequences of DNA has become...Biological Engineering in March is fused to a translational repressor. In principle, however, designer PUF...of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014...

  3. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ... emphasizes Accountability, Consistency, and Transparency. This label scores products by their environmental... but with the right incentives (for example, by grant agencies), we can shift toward a more sustainable...your RMV to the landfill, it’s collected and transferred to the Red2Green processing facility, where it...

  4. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    ...connection that many people honor. Talking with strangers is usually challenging but does get easier with...you might spend a whole day having to talk with strangers. Be bold in asking all kinds of people to connect...to share their stories. Thanks to the Benjamin Franklin Effect, most people actually enjoy helping (just...

  5. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ... LaFleur et al., 2019b). CHIME works through transduction of Cas9-expressing (Rosa26-Cas9) hematopoietic... a lentiviral vector containing a gRNA. These transduced stem cells can be implanted in irradiated recipients...four systems Name Function Plasmid Transduction Marker Mouse Strain C-CHIME Knocks out...

  6. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...pile of lab notebooks and binder on your desk. Arrange them in chronological order (or by project, if ...Organize you experiments, keep track of upcoming grant deadlines, and sync everything to your calendar....lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These programs can be...

  7. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...life repair genomic damage, specifically double strand breaks. Researchers have long taken advantage of...product is introduced into the cell by direct transformation. A given insert will typically contain both...selectable gene product for isolation of successful transformants. Addgene distributes several ready-to-use, modular...

  8. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ...data, more discussion, and more functionality. Transparency, both in what works and in what doesn’t, and...Ichise, H., Gola, A., Kabat, J., Lowekamp, B., Speranza, E., Croteau, J., Thakur, N., Jonigk, D., Davis...00644-9 Radtke, A. J., Kandov, E., Lowekamp, B., Speranza, E., Chu, C. J., Gola, A., Thakur, N., Shih, R...

  9. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...The system consists of dCas9, the mammalian transcriptional repressor Mxi1, and an optogenetic tool that...CRISPRi plasmids A multiplexed gRNA piggyBac transposon system for human pluripotent stem cells Lindy...and gRNAs whose expression varies depending on transgene design and delivery. They generated hPSC lines...

  10. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...historians have the most Twitter users. Biologists rank #8. Figure 1: Highly Represented Scientific Disciplines...Discipline. Biologists, neuroscientists, and ecologists (orange box) post tweets containing scientific domains ...network of life (green), physical (blue) and social (orange)  scientists largely cluster by discipline, suggesting...

  11. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ... Today, that changes. Today, Addgene launches a brand new Europe office located in London and operated...spondylarthritis, using Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study the expression...specialising in Molecular Biology. She has worked in a range of labs - cancer research, microbiology, virology...

  12. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ... the pores being opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive... survive when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody...where the eosinophils release cytotoxins via degranulation.  Antibody-dependent cellular phagocytosis (...

  13. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...of neuroscience. The larvae of zebrafish are translucent and allow for noninvasive live imaging with fluorescent...that allow charged particles to cross the cell membrane when exposed to light. Neuronal firing depends... Portugues, Ruben, et al. "Optogenetics in a transparent animal: circuit function in the larval zebrafish...

  14. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ... have pushed the boundaries in neuroscience by granting targeted control over neuronal activity. While... Mouse studies, however, require a permanent intracranial implant to be surgically installed to allow ...receptors Invasiveness Mice require permanent intracranial implant Noninvasive Controlling Stimulation...

  15. Fostering communication in remote collaborations

    Type
    Blog Post
    ...between my lab in Pennsylvania and a team in Paris, France. While efforts like these accelerate the progress...everyone can access data and drafts of manuscripts and grant applications in real-time so they can provide input...International Cancer Immunotherapy Conference in Frankfurt, Germany When funding and circumstances permit...

  16. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...or A:T->G:C edits in DNA without causing double-strand breaks. Our new page shows you base editing plasmids...certainly taken notice! Our newest page in the Transcriptional Regulation category already has five blue flame...with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation: Truncated gRNAs for Regulating ...

  17. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...better. More transcription factor binding sites in a promoter lead to higher transcriptional activation....for single molecule imaging, finding that plasma membrane-targeted CAAX-SunTag was 18-fold brighter than...

  18. Advice for Choosing a Research Project

    Type
    Blog Post
    ... research project establishes your professional brand. It offers colleagues insight into your interests...understand where the field is today? Have you talked to veterans in the lab or the field? What are possible pitfalls...how can you mitigate them? If you were to write a grant for this project, what would be your critical experiments...

  19. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...plasmids from the same group should not be co-transformed, so if you need two plasmids for an experiment... same incompatibility group should not be co-transformed. +Actual copy number varies. See below for ...stocks, agar stabs, or liquid cultures. Freshly transformed cells also give a higher plasmid yield that colonies...

  20. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ... synthesis companies often work within a narrow range for GC content. Rare codons also are not good predictors...sequences is Proteobacteria - the most frequent transfer of genes is from Proteobacteria to mammalian expression...natural gene sequence is, the less likely it is transferred into another organism in the lab. Predicting ...
Showing: 661 - 680 of 993 results