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We narrowed to 1,041 results for: ins

Showing: 661 - 680 of 1041 results

  1. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...that couldn’t package itself due to a few missing proteins. The envelope plasmid contained a viral envelope...lentiviral vector containing an antisense sequence against the HIV-1 envelope. As of 2013, 65 patients had...Lentiviral vectors in cancer immunotherapy. Oldham RA, Berinstein EM, Medin JA. Immunotherapy. 2015;7(3):271-84...

  2. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...concentration of a serially diluted standard is plotted against its OD560 measurements and the trendline y = 0.6561x...the minimum and maximum detection limits of the instrument being used to measure the response. The dynamic... to set yourself up for success!  Study the instruments and specific assays you will be using ahead of...

  3. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...be better suited for the job. As single domain proteins, they can be expressed in bacteria, making nanobodies...primary antibodies from different IgG subclasses. Instead, you can pre-incubate individual primary antibodies...Learn more about protein tags Read about E. coli strains for protein expression Additional Resources on...

  4. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...is introduced into the cap plasmid library by inserting short DNA sequences that contain all possible ...to 2-fold higher GFP intensity compared to the strains they were derived from. They also transduced a ...A cocktail of three AAV vectors expressing RGB proteins under the control of an inducer, i.e. tet-off ...

  5. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ... Cas13 enzymes do not contain the RuvC and HNH domains responsible for DNA cleavage, so they cannot directly...functional A->G change. They fused ADAR deaminase domains (ADARDD) to dPspCas13b, but observed low RNA editing...perform C-to-U edits, the authors note that it retains its adenine deaminase ability. The authors then...

  6. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...using water-oil emulsion technology. Each droplet contains the ingredients for amplification of the target...experiment. The process to titrate AAV by ddPCR begins with diluting the virus. It is important to note...containing approximately 20,000 droplets. Each droplet contains the material required for a mini amplification...

  7. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...Our Parent Teacher Association (PTA) has been instrumental in supplementing that curriculum. It’s through...little intimidating. Too simplistic and I would insult the students. Too complicated and I would quickly... Eric J. Perkins...

  8. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...credential from the Grant Professional Certification Institute (GPCI). GPCI evaluates a candidate's expertise...through the Grant Professionals Certification Institute, an Approved Trainer by the Grant Professionals... Social Media Strategist through the National Institute of Social Media. Follow Jo on Twitter @JM_Grants...

  9. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...love you back A successful lentiviral infection begins with your cells, both your packaging cells and ...However, viruses pseudotyped with other envelope proteins may have a limited number of targets and therefore...Finkelshtein, D., Werman, A., Novick, D., Barak, S., Rubinstein, M. “LDL receptor and its family members serve...

  10. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ... have since adapted it for a host of different proteins and small molecules from a variety of sample types...antibodies and thus are not suitable for low abundance proteins. Because the method uses a single antibody, it... and a series of wash steps removes non-target proteins and molecules from the assay. The user then adds...

  11. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...Members of the bacterial world produce an assortment toxins to claim territory or kill competing microorganisms...an efficient cloning tool. The plasmid pKSCTMF contains the mazF gene and a gene for Zeocin resistance... James Kaper used the hok/sok system to create strains that can stably express antigens in sufficient ...

  12. Bioinformatics at Addgene

    Type
    Blog Post
    ...well-supported, so we could focus on our development instead of building the entire ecosystem Proven performance...throughput necessary to keep up with our sequencing instrument (hundreds of plasmids each week!), we already...process. Similar to our first attempt, we manually inspected the output with a print statement at the end of...

  13. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...his or her chosen career path,” but individual institutions can have many different names for the positions...From Panel Session 2: Defining the Postdoc: An Institutional Perspective. This lack of complete information...Require that postdoc salaries be paid on fellowships instead of grants such that PIs no longer save grant money...

  14. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...for many labs. However, RNA interference (RNAi) remains steadfast in the background, a handy “oldie” as...cleaves the target mRNA or recruits other silencing proteins. Figure 1: Comparison of shRNA and miRNA...RNAi has retreated a bit to the background, it remains a solid option for altering the expression of specific...

  15. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ... each project and how useful each item is. For instance, if I don’t have draft blog post ready for publication...prevent you from making progress on a task. For instance, even if I had planned my day extensively, my ...that highlights the things you like about it. For instance, while I may have found splitting cells boring...

  16. Finding Your Perfect Job After University

    Type
    Blog Post
    ... a summer role as a Research Scientist at the Institute of Cancer Research in Sutton, located on the edge...and plenty of jobs in science at world renowned institutes. The lab at the University of Queensland required...field don’t really get to do a lot of lab work but instead really need to be comfortable with programming....

  17. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...targets, you might decide to do simultaneous IF instead (Figure 1B). To label all your targets in one step...yourself. If you can’t find a suitable antibody against your target protein, consider fusing a protein ...Neumann, E., De Caestecker, M., Gutierrez, D., & Spraggins, J. (2022). Multiplex Immunofluorescence on Fresh...

  18. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ... break, or is engineered not to cleave DNA and instead activates/represses gene expression by various ...minimum of 1,000 targeted cells to sequence. In this instance, a 5% off-target frequency is something to keep...-defective lentiviral vectors - which rely on insertion of DNA by the NHEJ pathway during DNA repair of...

  20. Deep Dive: qPCR

    Type
    Blog Post
    ...used GAPDH has noted issues as a reference gene (Higgins, 2000; Ref, Zhu, 2002), though there are cases ...run and the difference in CT values is plotted against the log input concentration for each dilution. ...://doi.org/10.1038/s41598-018-26707-8Suzuki T; Higgins PJ; Crawford DR. Control selection for RNA quantitation...
Showing: 661 - 680 of 1041 results