...the viral vector. This delivery method frees up space in the AAV allowing a researcher to fill it with...

...several ways to measure stickiness. In vitro, ultracentrifugation or gel filtration is the most common analytical...

...brightness of Tetbow allows this system to be used to trace neurons over millimeter-long distances across multiple...

...this commentary by Uhlen, et al. 2016 is a good place to start.) Reviewing available antibodies can seem...

...choose, your loading control measurement will take place either before or after blotting for your POI. ...

...Pooled Libraries to Generate Mutant Populations Use REPLACER Mutagenesis to Mutate Your Plasmids Resources...

...Joanne's "How to Choose the Right Next Lab or Workplace" On YouTube Joanne's 10 Things Scientists Ask ...

...expression vectors, the codon for the amino acid to be replaced with phosphoserine must be recoded to ‘TGA’ using...

...variables, we suggest these serotypes as a good place to start. Table 2: AAV tissue tropism for mice in...

...experiments, there is hardly any flexibility in gRNA placement (e.g., mutating a specific amino acid or introducing...

...2018). In this control, the primary antibody is replaced with pre-immune serum from the animal the antibody...

...optimized sequence will do little if it is in the wrong place, but because the target window is more-narrow, there...

...developing tropical country. This is why I set up a placement working in a rural health clinic in Ghana. My ...

...Douglas. Explorations in statistics: statistical facets of reproducibility. Adv Physiol Educ. 40(2): 248...

...bleach solution should be used to clean AAVs from workspaces. Speed: AAV transduction experiments are ...

...-target breaks Prediction algorithms: A good place to start for biased validation At the moment, many...

...continuous excitation. By generating PA-FPs fused to intracellular proteins, scientists can track single protein...

... used to ensure proper lysis, but you will still face column capacity limitations since the columns can...

...goal in the field of regenerative medicine is to replace lost or damaged cells. Here, I will discuss the...

...the trace file, you will see that these base calls are unreliable. This is an example of a trace file...with the sequence, trace file, and primer used. What program can I use to view my trace file? There are ...You should receive your sequencing results as a trace file (.ab1) which graphically depicts the sequence...four nucleotide bases. This is an example of a trace file from a high-quality portion of a sequencing...and include: The sequencing result The original trace file (.ab1 file) provided by your sequencing company...sequencing result, what should I do? Check your trace file first; the apparent mismatch/mutation may be... be the result of a mis-called peak in the trace file. If the mutation is not an artifact, please email...