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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 838 results for: ANG

Showing: 661 - 680 of 838 results

  6. The Strength of Story Telling

    Type
    Blog Post
    ...first author. When I raised this, he was pretty angry and started arguing with me. First I calmed the ...

  17. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...integration causing mutations and gene expression changes elsewhere in the genome. Second, expression is ...

  19. Ligation Independent Cloning

    Type
    Protocol
    ...transformation/replication process. LIC employs long overhangs to form a stable association between fragments...exonuclease functions, T4 DNA polymerase can create overhangs of varying length (typically 10-12 bp) based on...recognition sequence. This can create multiple distinct overhangs with a single enzyme, and remove the restriction...in subsequent reactions. Step 3: Create Vector Overhangs Treat the linearized vector with T4 DNA polymerase...accomplished by gel purification . Step 5: Create Insert Overhangs Treat your purified PCR product with T4 Pol in...
Showing: 661 - 680 of 838 results