We narrowed to 963 results for: APP
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TypeBlog Post...wanted to continue doing research, Doench went on to apply to graduate school at MIT. Doench excitedly remembers...Harlow’s group at Harvard Medical School and then applied to work at the Broad Institute: “The model that...
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Plasmids 101: Mammalian Vectors
TypeBlog Post...should die within 3-5 days and resistant colonies appear in about 10-14 days depending on how quickly your...culture to suppress bacterial growth, and is not appropriate for mammalian selection – do not confuse this... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post...methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) Most rely on transfection... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog Post...this need in the community, we approached it in the way Addgene approaches everything; we asked ourselves... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...repair, or replication, or any number of CRISPR applications and thought “OK, but how does this work in the...Chromosome Organization Keystone Symposium. As it happens, there are a lot of scientists who not only remember... -
Antibodies 101: Flow Cytometry Controls
TypeBlog Post...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...isotype control. Pro tip! Many companies will have appropriate isotype controls linked on their commercial antibody... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post...reactions by mixing this isolated plasmid DNA with appropriate sequencing primers, and hand deliver them to ...standards are met, we are taking a unique hybrid approach compared to our standard pipeline. For the first... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...faster deamination rate so that deamination can happen before Cas homologs let go. The Liu lab developed... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post...researchers (or your own from years ago!) do you really appreciate how few details are provided in most published...important enough to be answered and the methods are appropriate to answer those questions, the article is given... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog Post...Juan R. Torruella, U.S. First Circuit Court of Appeals. She served as in-house counsel at St. Elizabeth...responsible for bringing a systematic and disciplined approach to evaluating and improving the effectiveness ... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...TurboID and miniTurbo podcast segment A fundamental approach to studying membranes, organelles, and other subcellular...directed evolution to create APEX2. They have now applied the same directed evolution technique to the biotin... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...function relationships or, in more practical applications, allow a protein to be more effectively used...the creation of many new tools and potential applications. But this focus on development, along with the... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...not yet understood, one thing is clear: this application opens a lot of doors in neuroscience. The retrograde...retrograde serotype also has promising therapeutic applications; it can enhance therapeutic delivery across ... -
How Addgene Creates Content
TypeBlog Post...antibody and content team, we identified what applications should be included and what basic information...we usually make figures in BioRender or use appropriately licensed and cited figures from papers or image... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post... the appropriate ligand to activate the G proteins. It is estimated that there are approximately 950 different... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...product is then digested and ligated into an appropriate vector. Gibson Assembly, GeneArt Cloning, and...assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis, Trehan... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post...over or write to us to get them. Even though we’re happy to provide them, that never works on a large scale..., or words of advice for people who may want to apply this tagging system to their own work? Mendenhall... -
CRISPR 101: Non-Homologous End Joining
TypeBlog Post...Here, we’ll discuss NHEJ, and how it impacts what happens to Cas9-mediated DSBs in the genome. Non-homologous...also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR guides that flank regions of... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...design,” she said. “Before it was a little like tapping in the dark - a lot of trial and error.” The structural...analyzing the data. It should be straightforward to apply the new library for use in various cell types and...