We narrowed to 717 results for: CaS;

... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and...

...antibody (HCab), which are unique to camels, llamas, alpacas and other camelids. In terms of structure, HCabs...

...impacts the success of BiFC experiments. In either case, overexpression should be avoided because it can...

...binding by tweaking those parameters, but in some cases your antibody may just not be suitable for use with...

... infect the cell type of interest, which, in the case of Chan et al, was neurons and astrocytes. Why ...

...fluorescence upon binding to its cognate protein. In some cases this property eliminates the need for removing excess...

...regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then filtered based on two ...

...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out ...

...directly into your preferred buffer. If that’s the case, a buffer exchange can be performed using a desalting...

... blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere with protein folding HBH HHHHHHAGKA...

... series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP system enables...

...used to study protein-protein interactions. In this case, two portions of EGFP are fused to the proteins ...

...harbouring a Cre-dependent IL-1 beta overexpression cassette. Expression of Cre in these neurons turns on overexpression...

...both homeobox genes that are part of a genetic cascade that determines the cell fate of the dorsal mesoderm...

...inevitably have downtime and plenty of it. That’s not the case at an office job. I’ve frequently told job candidates...

...Nextflow for bioinformatics pipelining In many use-cases, including ours at Addgene, the overall purpose ...

...even make their own antibiotic compounds. In such a case, the microbe itself would be unharmed by the compound...

...the salary changes expected for postdocs in many cases should be as described in the notice of the new ...

...in a higher volume of lower titer virus. In such cases you may want to consider concentrating your viral...

... by looking at the different populations. In the case of Figure 2, one population is B- and another two...