We narrowed to 716 results for: CaS;

1. Antibodies 101: Producing Recombinant Antibodies

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   ...directly into your preferred buffer. If that’s the case, a buffer exchange can be performed using a desalting...

2. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   ...binding by tweaking those parameters, but in some cases your antibody may just not be suitable for use with...

3. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...impacts the success of BiFC experiments. In either case, overexpression should be avoided because it can...

4. Plasmids 101: Secondary Nanobody Toolbox

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   ...antibody (HCab), which are unique to camels, llamas, alpacas and other camelids. In terms of structure, HCabs...

5. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   ...fluorescence upon binding to its cognate protein. In some cases this property eliminates the need for removing excess...

6. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   ...regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then filtered based on two ...

7. Tips for arabidopsis transformation

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   ... blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic engineering...

8. AAVs in Retinal Gene Therapy

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   ... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and...

9. AAVs CREATed for Gene Delivery to the CNS and PNS

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   ... infect the cell type of interest, which, in the case of Chan et al, was neurons and astrocytes. Why ...

10. Lentiviral Vector Uses and Overview

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    ...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out ...

11. Plasmids 101: Protein tags

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    ... blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere with protein folding HBH HHHHHHAGKA...

12. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ... series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP system enables...

13. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ...used to study protein-protein interactions. In this case, two portions of EGFP are fused to the proteins ...

14. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    ... will save you a lot of headaches down the line! Cash in on gene synthesis Before you start your cloning...

15. Twenty Years of Sharing: Expanding Accessibility

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    ...Sinapse Biotecnologia 2022 Brazil   In some cases, the distributors broadly helped increase access...

16. Antibodies 101: The Four ELISAs and When to Use Them

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    ...process can be laborious and expensive. In some cases, conjugation methods can interfere with binding,...

17. Tips for Writing a Good Cover Letter

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    ... to three paragraphs. This is where you make the case for why you are a good candidate, explain how your...

18. Plasmids 101: Broad Host Range Plasmids

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    ...incorporate interchangeable antibiotic resistance cassettes (Prior et al. 2010), inducible promoters with ...

19. Antibodies 101: Reading a Flow Plot

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    ... by looking at the different populations. In the case of Figure 2, one population is B- and another two...

20. Hot Plasmids - November 2023

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    ...edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing efficiencies...