We narrowed to 772 results for: Dos;

...become resistant to? CRISPR may provide a method for doing just that. While challenges remain in the delivery...

...virus (similar to lentiviral vectors) because they do not encode all of the necessary viral proteins. Minigenome...

...protein molecules fail to correctly fold? Misfolded FPs do not fluoresce. A dark population is not readily apparent...

... list is a great place to start, the tables above do not delve into the tissue or development-specific...

... regions communicate with each other and how they do it (i.e. where the signals come from and what implications...migrates through neurons where it can replicate and by doing so spread across several synaptic connections (Ugolini...

...to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of dCas9. The...editors often produce a mixed population of edits, ABEs do not display significant A to non-G conversion at ...

...total plasmid DNA. Tips and Troubleshooting What do I do if my transformation efficiency is not high enough... Pooled Libraries Molecular Biology Reference How do I process my Addgene pooled library? Introduction...Endura from two separate transformations). Pro-Tip Do not pipette repeatedly or mix when removing SOC containing...Critical Be careful not to rip or shred the agar. Do so by gentle spreading. Some spreaders have a sharp...Maxi Kit (one conical is its own Maxiprep). Critical Do not freeze pellets for later purification. Immediately...colder reagents (not including the recovery media!). Do not proceed with Maxipreps or NGS until adequate ...representation is maintained. Maxipreps - Less is more: Do not overload the Maxipreps as yield can dramatically...

... given sequence. You want to choose enzymes that: Do not cut within your insert. Are in the desired location...(usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid. Bonus: It is helpful...GAATTCATGTGGCATATCTCGAAGTAC-3'. Many restriction enzymes do not cut DNA efficiently at the end of a linear piece...any 6 bases, but you should ensure that the bases do not result in the formation of a hairpin structure...empty plasmid, you will still have colonies when you do not add ligase. If the colonies are a result of recipient...significantly more colonies when you add ligase. If you do not see any colonies, you should conduct a positive...

...more. Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that should...separate non-integrating episomes to allow sorting and dosage tracking of reprogramming factors Fluorescent tagged...

...want to choose enzymes that: Flank your insert, but do not cut within your insert Are in the desired location...(usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Will result in your...you cannot find enzymes that meet these criteria, do not fear. You have other options, such as: Adding...empty plasmid, you will still have colonies when you do not add ligase. If the colonies are a result of recipient...significantly more colonies when you add ligase. If you do not see any colonies, you should conduct a positive...

...little excess gel around the band as possible. To do so, it is often important to take the excised band... about DNA quantification here . Tips and FAQ How do you get better resolution of bands? A couple simple...gel comb; or c) loading less DNA in the well. How do you get better separation of bands? If you have similarly...

...clinical grade hiPSCs, reprogramming methods that do not involve modification of the original genome by...

...science.1138389 Le TTH, Tran NT, Dao TML, Nguyen DD, Do HD, Ha TL, Kühn R, Nguyen TL, Rajewsky K, Chu VT ...off-target editing, and, for RNA editing, fine-tuning dosage so that enough wild-type MECP2 is made inside the...

...find a positive one. So if you have better things to do than clone picking, choose your guide RNAs wisely...

...saturation mutagenesis with the same protocol. What do you think about one pot saturation mutagenesis? How...

...allowing co-imaging. And because bioluminescent markers do not need to be excited by an external light source...

...biorender.com.   Of course, this is quite easy to do on a plot generated in Biorender! While a helpful...

...we can tell, DnaA doesn’t need to hydrolyze ATP to do its job — although ATP does get hydrolyzed over time...

...machinery such as Rep or other initiator proteins, they do not require host proteins for replication and can...spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas...

...camera’s image to my remote location, live. After doing a bit of looking, I found an inexpensive HDMI transmitter...