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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,030 results for: ELL

Showing: 661 - 680 of 1030 results

  1. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post
    ...process will begin. Partnering with seqWell, we are using the plexWell technology to easily and quickly ...ever worked to sequence DNA on NGS platforms will tell you, while the process has become much more refined...assembly process. Again, thanks to our partners at seqWell we make use of a pipeline that takes the raw data...necessary adapter sequences for Illumina sequencing as well as unique barcodes per sample so we can assign sequencing...Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020) A Novel Next-Generation...

  2. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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    Blog Post
    ...Communications, Harvard University Ray Howell -Founder of Howell Communications Philip Brenner, PhD - Assistant...scientific enterprise and the way it is funded have been well documented over the past 2 years (Alberts et al,... to shape the sector throughout their careers (McDowell et al, F1000Research, 2014; Dolan, F100Research...relationship, along with, of course, union dues. Time will tell the extent of the implications of this ruling and...27175853. Pubmed Central PMCID: PMC4866822. 3. McDowell, Gary S., et al. "Shaping the Future of Research...

  3. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    Blog Post
    ... acid (DAP), a component of the cell wall. Once in the recipient cell, the CRISPRi DNA (purple) flanked... is propagated during normal DNA replication and cell division. Mobile-CRISPRi does not disrupt gene function...mobilize these plasmids for transfer to recipient cells during a tri-parental mating scheme. Mobile-CRISPRi...bacteria associated with human disease (S. aureus, Klebsiella pneumoniae, and P. aeruginosa) in addition to...antibiotic trimethoprim. When Enterobacter aerogenes, Klebsiella pneumoniae, and P. aeruginosa (all opportunistic...

  4. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    Blog Post
    ...AmpliCan We chose to adapt Crispresso by Luca Pinello and Kendell Clement for batch analysis because of its...Leonetti Ryan Leenay Jason Li Max Haeussler Luca Pinello Kendell Clement Andy May Additional resources on the...members have taken up to a month to design a single 96-well plate for HDR that satisfy all requirements. To ...all the standard information that goes into a 96-well plate and comes out of it. Biotech companies are...

  5. Degrading DNA with Cascade-Cas3

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    Blog Post
    ...there isn’t a template strand left behind that the cell can use to repair. Figure 3: General overview...Occasional cleaving of the target strand is not as well understood but likely occurs in similar regions....complex in nucleotide unwinding steps. Molecular Cell, 70(3), 385-394.e3. https://doi.org/10.1016/j.molcel...formation by a type I-C CRISPR Cascade. Molecular Cell, 83(5), 746-758.e5. https://doi.org/10.1016/j.molcel...broad and unidirectional genome editing in human cells. Nature Communications, 10(1). https://doi.org/10.1038...CRISPR–Cas systems for genome engineering in human cells. Nature Biotechnology, 37(12), 1471–1477. https:...

  6. Antibodies 101: Flow Compensation

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    Blog Post
    ... make it difficult to properly gate your desired cell population. By compensating between fluorescent ...online manual for your cytometer should be able to tell you the laser configuration. In general, if two ...Figure 1A). Applying compensation means you are telling your cytometer to adjust the fluorescent signals... your fluorescent markers are staining for. If a cell is positive for both markers, it will show up as...Figure 3: Flow plot for a double positive sample. The cell population in the top right corner of the plot is...learning! Ashlyn has a research background in CD8+ T cells and cancer immunotherapies. She is currently a research...

  7. Anatomy of a Plasmid Page at Addgene

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    Blog Post
    ...different kinds of information on our plasmid pages? Well, to help make the most of these pages, we've written...Addgene’s plasmids is an indication of popularity. A yellow flame indicates that a plasmid has been ordered...process and these can be found in this location as well. These maps make it easy for scientists to see if...analyzed using Addgene’s Analyze Sequence tool. Excellent, how can I check if the plasmid will be within...resistance, and optimal growth temperature are listed, as well as the name of the strain.  Most Addgene plasmids...such as some of our viral vectors, in NEB Stable cells. Resource information: This section contains information...

  8. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ...favorite parts for plant synthetic biology Addgene: Tell me about the Golden Gate Modular Cloning (MoClo)...really easy to clone genes for expression in plant cells. Sylvester and I chose the 96 most useful things...used as we have tested them to show that they work well in plants and give similar stability to transcripts... parts available in your lab - if you get the 96-well kit - everything is so easy. We have undergrads ...a real person, so that students see academics as well-rounded human beings. It’s also a great communication... that want to have a big impact on the world and tell them to work in plant science. I think their chances...

  9. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post
    ...CRISPR complex much easier in various cells, including primary mouse cells. We believe that this system will...In 2014, you joined Addgene's Advisory Board, as well as the Editorial Board of Scientific Reports and...US, I very much enjoyed interactions with many excellent colleagues at NYU and visiting scientists, such...In addition, I've been very lucky to work with excellent colleagues in my lab. On the other hand, I feel...good for Japanese science. I really miss the intellectual environment in the US in this regard. In addition...

  10. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    Blog Post
    ...occurs when bacterial cells regulate gene expression in response to changes in cell density. At the mixer...cohort of friends and colleagues had moved on as well, so I knew very few people. I didn’t really fit ...Singletons gained from spending time with a mentor, as well as having a cohort experiencing similar situations...introduced to several people by their mentor, as well as their bin-mates.  They liked us. They really ...Hendrickson, Jonathan Jacobs, Mo Kaze, Christina Kellogg, Heather Martinez, Pat Schloss, Tara Smith, Marc...

  11. Lab to Office Culture Shock

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    Blog Post
    ... it, and many of my scientist colleagues have as well. Making the transition from the bench can be jarring...scientist going through even the worst experimental dry spells. Losing that sense of discovery can be hard since...place. The feeling of camaraderie one gets from a well-functioning lab team. Obviously not everyone gets...applying. I probably did not respond to her question as well then as I would now. I would have to repress laughter...the right set of tools to help you do your job as well as possible. It’s in their best interest to do so...the newer members of our Scientist team says, “a well-run meeting where everyone has a voice and the group...

  12. Plasmids 101: Gateway Cloning

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    Blog Post
    ...choose a molecular cloning technique that will work well with a given set of resources, time, and experimental...the next step is to transform competent E. coli cells and select the positive clones. The entry clone ... expression of the human KRAS gene in mammalian cells. STEP 1: Generate an Entry Clone There are a few...ccdB gene. Since the ccdB product is toxic to the cell, Gateway cloning efficiency can reach >99%. ...digest! Then, you can transform or transfect the cells that you want to use for your experiments and verify...Phage (Lambda) and Higher Organisms (Cambridge, MA: Cell Press). Additional Resources on the Addgene Blog...

  13. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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    Blog Post
    ...editing reagents into cells. Viral vectors, because of their natural ability to infect cells, have emerged as...biologist, academic, and researcher. Among the most well-known CRISPR-associated nucleases are Cas9 and Cas12a...which poses a challenge for efficient delivery into cells, particularly via viral vectors. While viral delivery...for gene editing and allows targeting of specific cell types, it has a limited capacity to carry large ...biolistic, or PEG-mediated transformation into plant cells. Conclusion & Future Directions TnpB represents...

  14. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    Blog Post
    ...recommend transforming into chemically competent cells in a 96-well PCR plate. Always confirm your results Congratulations... sequences of the parts and acceptor vectors, as well as the restriction enzymes, and it will create the...transfer and the restriction–ligation reactions in a 96-well (or higher!) format. Liquid handling robots are ...Dr. Rob Hurt for high-throughput minipreps in 96-well plates. Plate wisely You will likely see fewer numbers... this, you can increase the volume of competent cells you plate after each transformation and/or use different...

  15. CRISPR 101: Any Base Transversion Editors

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    ... edit A → Y (Y = T or C) in 67.17% of screening cells (Tong, Wang, et al., 2023). Based on this promising...the base editor. Black arrows indicate natural cellular processes. Created with BioRender.com. The...Cas9 nicks the non-edited strand to encourage the cell to repair the DNA using the edited strand as a template... efficient A•T-to-C•G base editing in mammalian cells and embryos. Nature Biotechnology, 42(4), 638–650...programmable T-to-G and T-to-C base editing. Molecular Cell, 84(7), 1257-1270.e6. https://doi.org/10.1016/j....efficient T-to-G and C-to-G editing in mammalian cells. Nature Biotechnology, 42(10), 1538–1547. https:...

  16. Hot Plasmids: Fall 2024

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    Blog Post
    ...(or even in the middle of proteins) and performs well in many different antibody-based applications.  ...Randall, R. E., Young, D. F., Goswami, K. K., & Russell, W. C. (1987). Isolation and characterization of...useful addition to the toolkit for visualizing cellular processes in C. elegans: mScarlet3 for applications...biologically relevant forces at focal adhesions in living cells in several different contexts.   Figure ...substrate furimazine. Right: Demonstration of PILATeS. Cell adhesion to a PILATeS-functionalized coverslip creates...minutes.  Find PILATeS plasmids here! Zhong, B. L., Elliot, J. M., Wang, P., Li, H., Hall, R. N., Wang, B....

  17. Top Requested Plasmid of 2017 - pMD2.G

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    ...used to deliver Cas9 and other genes to mammalian cells. For this reason, the top requested plasmid of 2017... coat the virus and help determine the types of cells that it can infect. The VSV-G envelope is widely... lentiviruses to infect many different types of cells. This means researchers who use VSV-G can deliver...deliver their genes of interest to a variety of cells and study their effects. To date, nearly 900 articles...in studies of the secretome, ubiquitin ligase, cellular senescence, autophagy, CRISPR, and so much more...

  18. An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

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    Blog Post
    ...fluorescent proteins as scaffolds for cell-specific gene manipulation.” Cell 154(4) (2013): 928-939. PubMed ...Cepko Interested in Working on the Eye 9:05-10:16 : Cell Targeting in Retinal Gene Therapies 10:17-14:34 ...28:39 : Methods for Delivering Multiple Genes to Cells for Gene Therapy 28:40-35:10 : GFP Activatable Tools...publication at Addgene. 2. Tang, Jonathan C.Y., et al. “Cell type-specific manipulation with GFP-dependent Cre...

  19. Transferable Skills Guide: Conflict Resolution

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    Blog Post
    ...other people, but resolving your own conflicts as well. What I really brought to the table was empathy,...you can identify them in yourself and learn how to sell them. Types of Conflict Before explaining my approach...occurs when people or teams who normally get along well with each other disagree about a specific decision...good listener absorbs and remembers and makes the teller feel heard. I am not always a stupendous listener...will accept. If you are able to broker a compromise well, you can help everyone walk away from a conflict...solution C. And if a conflict resolver does their job well, not only will solution C be better than either ...

  20. CRISPR 101: Homology Directed Repair

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    Blog Post
    ...double-strand break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen...repair fix DNA double-strand breaks?  In eukaryotic cells, repair of DSBs occurs primarily by two pathways...used, however, these are generally more toxic to cells than their plasmid counterparts. Another consideration...how to fix a broken relationship. Pardo B, et al. Cell Mol Life Sci (2009). PubMed. Regulation of DNA...break repair pathway choice. Shrivastav M, et al Cell Research (2008). PubMed. Mechanisms of DNA double-strand...Improve HDR Efficiency for CRISPR Editing in Human Cells Resources on Addgene.org Browse All CRISPR Plasmids...
Showing: 661 - 680 of 1030 results