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...when enhancers were active or silent, they could identify the target genes of previously uncharacterized...has made targeting much easier! With the help of guide RNAs, fusions between Cas proteins and the epigenetic...this fusion protein allows for LSD1 targeting to a wider set of genomic targets. These two vectors are part...CRISPR-based DNA demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair... the Addgene Blog Cas9 Activators: A Practical Guide CRISPR Between the Genes: How to Experiment with... Resources on Addgene.org Check out Our CRISPR Guide Pages Find Validated gRNAs to Target Your Genomic...

...Watakabe et al., 2015) (Figure 1). You’ll need to consider if such properties could help, or harm, your experimental...tools, where modification of cellular activity outside of your experiment site or circuit can occur. Promoters... promoters  This type of promoter allows you to decide when to turn on, or turn off, gene expression by...promoters to begin testing, you’ll next need to decide how much virus to put into your system, which is...injection volume.  There are a few factors to consider. First, the titer required is inversely related... posts in our Viral Vectors 101 series can help guide you with these, so keep reading! Good luck, and...

... coming out of MITs Human Dynamics Laboratory identified five characteristics of very successful teams...this series). Members carry on back-channel or side conversations within the team. We are not talking...contribute. Members periodically break, go exploring outside the team, and bring information back. No team ...colleague: MBWA "Manage By Walking Around" – don't hide in your office. Stop by instead of emailing.    ...volunteer constructive feedback and suggestions. Consider offering rewards (gift card, afternoon off, etc...

...mostly because of this article: “A systematic identification and analysis of scientists on Twitter.” This...Scientific Disciplines on Twitter. Twitter users were identified as scientists if they had a scientific title ...scientists within their fields than scientists outside their fields Figure 6: Follower Network of Scientists...follow scientists within their field rather than outside their field. Lower: Follower networks of subgroups... Y., & Sugimoto, C. R. (2017). A systematic identification and analysis of scientists on Twitter. Plos...

... a very different schedule. Fortunately, I was provided with a detailed training schedule that helped ...it relates to other teams at the company. This provides new (and old) employees with information on how...possible to work the remainder of the three hours (outside of the core hours) at a time that works for you...transition into a traditional work environment was aided by the efforts being put in by Addgene to ensure...blog.      More resources on the Addgene blog A Guide to Getting Started in Undergrad Research Tips for...

...requesting and ordering. This split allows us to provide researchers with increased choice and flexibility... Plasmids available for Packaged on Request consideration will have a banner on their material page. Interested...testing, and viral genomic sequencing to confirm the identity of the packaged DNA and serotype. Please note ... in-stock preps. If a request has technical considerations that may affect expected titers, a customer...1–2 days (USA); 3–4 days (international; varies widely by country and/or distributor.)  You should plan...

...certainly hope not! One of our core values here is to provide unmatched customer support, as this is vital to...exact problem, and it’s called “self-service.” The idea here is to build features that allow users to help...general question or concern about the answers we provide, within the widget you also have the option to ... we’ll be continually improving the support we provide based on your valuable feedback. This is Addgene...first. Resources on the Addgene Blog Read other Inside Addgene Posts Learn about New Features on Our Plasmid...

...joined by Addgene Scientist Matt Ferenc (one of our resident CRISPR experts) at the 2014 Johnson & Johnson’...up to the discovery of the mechanism of dual-RNA-guided DNA cleavage in adaptive bacterial immunity and...Dounda and Charpentier met at a 2011 conference and decided to collaborate on what was a “curiosity” driven...thanked Martin Jinek and Krzysztof Chylinski (who coincidentally speak the same Polish sub-dialect making their... Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale of survival and evolution...

...Visit our viral vectors guide Find our chemogenetics and optogenetics science guides ...Chemogenetic approaches excel at broad tissue or system-wide targeting, as the ligand can reach target cells ...optogenetics. If broad control of whole circuits or tissue-wide systems is necessary, use chemogenetics. Note that...approaches suffer from unique sets of off-target side-effects that should be carefully controlled for ...

...post-grad plans, but in the end, writing won out. “I considered teaching at a liberal arts school but I’ve always...Iowa, but kept up a freelance writing hustle on the side. “I actually stumbled upon, about 6 months in, this... was for Cactus Communications, a company that provides scientific editing services. “I was working during...At the end of her postdoc, Caitlin had a clearer idea of her career goals: “My plan was to go into editing...landing her job. All of the hard work she’d put in outside the lab had paid off. “The combination of the editing...

...biosensors When choosing a biosensor, you first need to decide the best way to track or measure the process you... of small molecules like arabinose or hydrogen peroxide or ions like calcium and zinc. Some biosensors...compatible with your lab and experimental setup? Consider what kind of instrumentation you have available...components of individual cells Separation/identification of fluorescently-labeled components Fluorescence-activated...Optogenetics Plasmids Visit the Fluorescent Protein Guide Pages ...

... mapping where a protein binds genome-wide, and has been widely applied in many model organisms and cell... Enter CUT&RUN, an enzyme-based protocol for identifying DNA binding patterns that overcomes some of the... degree of signal to noise is desired. It also provides an excellent additional measure of DNA binding...technique will quickly become a standard method alongside ChIP. Many thanks to our guest blogger, Matthew...

...clinical data about a disease or therapeutic Side-by-side comparison of the same experiment from different...makes your insights discoverable. Watch this quick video to learn how ReFigure works and continue reading...Apps to Save Time and Track Your Experiments Use video to share your science at We Share Science Learn ...with Addgene Find Molecular Biology Protocols and Videos Find Other Molecular Biology Resources ...

...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...a new CRISPR screening method that makes use of guide RNAs with internal barcodes (iBARs) in their loop...experimental replicates for each of the gRNAs. The idea was to assign four different barcodes to each of...covers every annotated human gene, it enables genome-wide screening with a high MOI, which is beneficial for... and can serve as a drug-screening platform to identify new antifungals that target calcineurin. Current...

...important to use a high fidelity polymerase to minimize mutations. The fidelity of the polymerase becomes...as Addgene’s Sequence Analyzer, to allow you to identify which restriction sites are present in a given...compatible with a standard PCR reaction (see PCR Video). Therefore, our Forward Primer will use the sequence... chance for mis-priming, so you can use a pretty wide range of annealing temperatures, but you may need...

...demonstrate that 85% of leaving postdocs pursue a career outside the traditional academic silo to tenured professor....  Here are some questions and perspectives to consider. What do academic and non-academic careers in ... a greater sense of autonomy - there are fewer outside influences on your topics of research or course...move around every 3-6 years—a short and exciting ride Complicated matrix structures are common Small... a scientist you enjoy the most. Having a better idea of where you want to go will help you focus on the...

..., Addgene will have unique Research Resource Identifiers (RRIDs) for each antibody, making it easy for...topics are discussed on Addgene’s blog and Antibody Guide, which include information about immunofluorescence...suggestions about how to make the resource better or ideas for new materials to add to the collection, please...find useful antibodies for your research. Please consider sharing your experiences with antibodies from ...Introduction to Immunofluorescence Addgene’s Antibody Guide The project is supported by a NIH BRAIN Initiative...

...transformation fall into two main categories: antibiotic/herbicide resistance and fluorescent proteins. Selection...undifferentiated plant cell culture), which is great for a wide variety of applications. But some techniques require...CRISPR-Cas9 gene editing, transformation may be identified by resistance to an antibiotic. Successful editing...therefore, are useful for research, and they are identified by a phenotype caused by the segregating mutation...these mutant plants. Using RUBY to simplify the identification of these mutants will speed up my work and ...

...developed and tested by ABRC staff. These kits provide in-depth, ready-to-teach lab protocols and supporting...supporting materials such as instructional videos and datasheets. The remaining kits were donated to ABRC...cycle, and is relatively easy to grow, it is an ideal organism for teachers looking to expand their science...outlined in ABRC’s kits vary by module. As a general guideline, experiments that include growing plants require...available via e-mail and phone to answer questions and provide support. Growing capacity - ABRC outreach today...

...service that we, as scientists, provide for journal editors to help decide whether work is suitable for ...of the 18th century. Yet the peer-review was not widely adopted by the scientific community until the mid...peer-reviewing journal submissions since 2015 for a wide variety of journals. Since then, I developed a routine...submitted paper in addition to the journal-specific guidelines.  Peer-review step 1: The first read At the first...