Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 1,027 results for: LEA

Showing: 661 - 680 of 1027 results

  1. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...your questions, data, and conclusions should be clearly identified and interpreted in less than a couple...to read. The key to the success of this style is clear borders around your columns, and good spacing between...used for displaying all of one’s figures, or at least the majority of the visual data. This central box...

  2. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control...Self-complementary AAV mediates gene targeting and enhances endonuclease delivery for double-strand break repair." Gene...

  4. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...one is in the making by Dr. Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated...these criteria and deemed positive, it will be released through the website for public reference. The ... sending antibodies, and/or working with Dr. Wu please contact us here! Ava Shipman is a research assistant...

  6. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...receptors hijack cell signaling pathways and can lead to such responses as decreased heart rate (Redfern...properties such as endogenous gene expression, which can lead to changes in normal cell physiology and function...posts about neuroscience Resources on addgene.org Learn more about chemogenetics in our chemogenetics guide...

  7. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...the different SARS-CoV-2 spike variants. You can learn more about their research in this article. To see...that are codon optimized. Browse the collection or learn more about it on the blog. COVID-19 research is ....0030 Additional resources on the Addgene blog Learn more about mapping the SARS-CoV-2 - human protein-protein...

  8. CRISPR in the Clinic

    Type
    Blog Post
    ...cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic potential...of their disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult...Early-stage trials showed a high success rate in clearing misfolded protein levels as measured in the blood...

  9. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...analyzes publications and figures using machine learning to extract important features such as influential...you organize and search for papers later. Pro tip Learn more about authors by exploring author pages (for...literature. Additional resources on the Addgene blog Learn why scientists should be on Twitter Read our Early...

  10. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ... “Download Deposit Spreadsheet” (see Figure 1). Please read our “How to Deposit Your Plasmids with Addgene... a collection that is greater than 75 plasmids, please contact us at [email protected]. These deposits...37 °C, or room temperature. Growth Strain Please indicate which growth strain Addgene should propagate...

  11. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...Our Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has...Lineage Tracing Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral...

  12. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...Having never been to China myself, I was pleased to be able to participate when the organizers of the...dozens of different mushroom varieties alone. At least I could tell that they were mushrooms. In some cases...Medicine Initiative and how this initiative will lead to advances in human health. It was inspiring to...

  13. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...preparation of the fiber optics is probably the least difficult portion of optogenetics (though it does...polishing. Efficiency of the implant should be at least 85% of the initial laser output. The same considerations...University.  His interests include the role of learning and memory in opioid addiction and science communication...

  14. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...account, business cards, incorporation, laboratory lease, etc. I still flip to that page in my lab notebook... into executing a successful business that the learning curve is pretty steep. But we can rely on one ... about this transition (so far) and what we’ve learned.  Thank you to our guest bloggers! Nikolai Braun...

  15. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ... are nuclear- or DNA-binding proteins, you may need to sonicate your lysates in order to release the proteins...ladder on your gel and keep an eye on it and the leading line (dye front) visible in the sample buffer. ...stripping and reprobing. Just make sure to go from least to most abundant protein if you’re stripping, since...

  16. Pooled Library Amplifications

    Type
    Blog Post
    ...pooled library with Addgene, you may be surprised to learn that we ask for an amplification protocol with your... repeated amplifications of pooled libraries can lead to issues such as recombination and loss of plasmid...Barcode Watermelon Library 5,000,000 30 bp At least 200 ng Liquid Electroporation Protocol (DOCX...

  17. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... Resources on the Addgene Blog Learn about inducible promoters Learn about repressible promoters Check...the addition of post-translational modifications Learn more molecular biology in our Plasmids 101 eBook...

  18. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...conference If there’s a cool new method that you learned about at the talk, bring it to your next lab meeting...fruitful. After the conference Bring back what you’ve learned from the poster session to your lab. Perhaps some...scientific communication, and scientific networking Learn how to set up and informational interview and Expand...

  19. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...FPs to highlight various subcellular structures. Please see Figure 1 for commonly used markers for major...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...Microscopy. Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous Gene Tagging ...
Showing: 661 - 680 of 1027 results