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We narrowed to 1,015 results for: LEA

Showing: 661 - 680 of 1015 results

  1. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...final recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide webpage, and references...purified plasmid stock should be analyzed by endonuclease restriction digest or fully sequenced to confirm...a week), and do NOT harvest the cells before at least 10 days of incubation as it will result in very ...

  2. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    Blog Post
    ...Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control...Self-complementary AAV mediates gene targeting and enhances endonuclease delivery for double-strand break repair." Gene...

  3. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...Our Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has...Lineage Tracing Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral...

  4. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...Having never been to China myself, I was pleased to be able to participate when the organizers of the...dozens of different mushroom varieties alone. At least I could tell that they were mushrooms. In some cases...Medicine Initiative and how this initiative will lead to advances in human health. It was inspiring to...

  5. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...preparation of the fiber optics is probably the least difficult portion of optogenetics (though it does...polishing. Efficiency of the implant should be at least 85% of the initial laser output. The same considerations...University.  His interests include the role of learning and memory in opioid addiction and science communication...

  6. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...account, business cards, incorporation, laboratory lease, etc. I still flip to that page in my lab notebook... into executing a successful business that the learning curve is pretty steep. But we can rely on one ... about this transition (so far) and what we’ve learned.  Thank you to our guest bloggers! Nikolai Braun...

  7. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ... are nuclear- or DNA-binding proteins, you may need to sonicate your lysates in order to release the proteins...ladder on your gel and keep an eye on it and the leading line (dye front) visible in the sample buffer. ...stripping and reprobing. Just make sure to go from least to most abundant protein if you’re stripping, since...

  8. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    Blog Post
    ...FPs to highlight various subcellular structures. Please see Figure 1 for commonly used markers for major...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...Microscopy. Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous Gene Tagging ...

  9. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...conference If there’s a cool new method that you learned about at the talk, bring it to your next lab meeting...fruitful. After the conference Bring back what you’ve learned from the poster session to your lab. Perhaps some...scientific communication, and scientific networking Learn how to set up and informational interview and Expand...

  10. Pooled Library Amplifications

    Type
    Blog Post
    ...pooled library with Addgene, you may be surprised to learn that we ask for an amplification protocol with your... repeated amplifications of pooled libraries can lead to issues such as recombination and loss of plasmid...Barcode Watermelon Library 5,000,000 30 bp At least 200 ng Liquid Electroporation Protocol (DOCX...

  11. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... Resources on the Addgene Blog Learn about inducible promoters Learn about repressible promoters Check...the addition of post-translational modifications Learn more molecular biology in our Plasmids 101 eBook...

  13. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ... in the primary literature, and as a result can leave scientists under-informed with respect to how different...the year, those that meet our minimum standards (clear and detailed methods and image descriptions, a minimum...computer files. If you asked your team members or colleagues: How much unpublished AAV data do you have sitting...

  14. Tips for arabidopsis transformation

    Type
    Blog Post
    ...fantastic model organism for many reasons, not the least of which is ease of transformation. There are many...finding true transformants more difficult. Don’t leave your plants on selective plates for too long: even...recovered from the stress of selection you can clip a leaf and extract genomic DNA for genotyping. This won...

  15. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...commonly result in a complete loss of function while nuclease-deactivated Cas9 (dCas9) can be tethered to a ...which contains multiple point mutations in the nuclease domain. These mutations result in an inactive ...about CRISPR Kinome Libraries available at Addgene Learn about the advantages of pooled CRISPR libraries ...

  16. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...retroviruses. Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing ...Third-generation plasmids can be packaged with either system. Please note that the generation used to package the virus...Addgene Blog: Download our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus...

  17. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... variety of expression systems. Keep reading to learn more about the Gateway cloning method and its advantages...conditions, the attL and attR sites can recombine, leading to the excision of the phage from the bacterial...Resources on the Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony...

  18. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...contributed by the Mesothelioma Cancer Alliance. We have learned much about the causes of cancer and the different...nucleus, if the cell dies from injury, this protein leaks out into the cytoplasm, migrates out of the cell...About Cancer and Addgene's Cancer Collection Pages Learn How the ClonTracer Library Can be Used to Study ...

  19. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...been demonstrated in zebrafish using zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another...zebrafish zygote transforms into an organism with a clearly established body plan including head, eyes, and... tracked in whole brains of swimming, actively learning larvae, providing unprecedented views into cognitive...

  20. Bioinformatics at Addgene

    Type
    Blog Post
    ...much trial and error to learn the scripting language, but our path forward was clear: to build one process...assembly algorithms meant that we already had a clear idea of the necessary steps to perform:    ...
Showing: 661 - 680 of 1015 results