Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 1,028 results for: LEA

Showing: 661 - 680 of 1028 results

  1. Engaging with science and society at pgEd

    Type
    Blog Post
    ...Washington DC; convening meetings of experts and leaders from academia, industry, community, education and...the biological sciences, as well as to acquire leadership and communication experience. These included ... science career path Resources on Addgene.org Learn how Addgene engages with the community on our Addgene...

  2. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...one is in the making by Dr. Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated...these criteria and deemed positive, it will be released through the website for public reference. The ... sending antibodies, and/or working with Dr. Wu please contact us here! Ava Shipman is a research assistant...

  5. A Look at Addgene's QC Process

    Type
    Blog Post
    ... high-fidelity polymerases can make mistakes and lead to the accumulation of errors in DNA sequences. ...originally intended. Sharing unverified plasmids can lead to far reaching consequences, from wasting time ...available for distribution. In these cases, the QC team leaves a note on the plasmid page to make requesting scientists...

  6. CRISPR in the Clinic

    Type
    Blog Post
    ...cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic potential...of their disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult...Early-stage trials showed a high success rate in clearing misfolded protein levels as measured in the blood...

  7. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...Genes allowing bacteria to survive toxins are the clearest example of a plasmid benefiting its host. For ...engineered entirely new types of plasmids without clear analogues in nature. For example, helper plasmids...plasmid tools available in the Addgene repository. To learn more about some other common plasmid features, such...

  8. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...reprobing, maximize your signal by starting with the least-abundant protein and working toward the most abundant...primary and secondary antibodies from your membrane, clearing the way for the second blot. There are multiple...with the mild one. Reprobe Once your membrane is clean of antibody, you’re ready to repeat your western...

  9. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...receptors hijack cell signaling pathways and can lead to such responses as decreased heart rate (Redfern...properties such as endogenous gene expression, which can lead to changes in normal cell physiology and function...posts about neuroscience Resources on addgene.org Learn more about chemogenetics in our chemogenetics guide...

  10. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...final recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide webpage, and references...purified plasmid stock should be analyzed by endonuclease restriction digest or fully sequenced to confirm...a week), and do NOT harvest the cells before at least 10 days of incubation as it will result in very ...

  11. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control...Self-complementary AAV mediates gene targeting and enhances endonuclease delivery for double-strand break repair." Gene...

  12. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ... “Download Deposit Spreadsheet” (see Figure 1). Please read our “How to Deposit Your Plasmids with Addgene... a collection that is greater than 75 plasmids, please contact us at [email protected]. These deposits...37 °C, or room temperature. Growth Strain Please indicate which growth strain Addgene should propagate...

  13. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...Our Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has...Lineage Tracing Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral...

  14. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...Having never been to China myself, I was pleased to be able to participate when the organizers of the...dozens of different mushroom varieties alone. At least I could tell that they were mushrooms. In some cases...Medicine Initiative and how this initiative will lead to advances in human health. It was inspiring to...

  15. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...preparation of the fiber optics is probably the least difficult portion of optogenetics (though it does...polishing. Efficiency of the implant should be at least 85% of the initial laser output. The same considerations...University.  His interests include the role of learning and memory in opioid addiction and science communication...

  16. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...account, business cards, incorporation, laboratory lease, etc. I still flip to that page in my lab notebook... into executing a successful business that the learning curve is pretty steep. But we can rely on one ... about this transition (so far) and what we’ve learned.  Thank you to our guest bloggers! Nikolai Braun...

  17. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ... are nuclear- or DNA-binding proteins, you may need to sonicate your lysates in order to release the proteins...ladder on your gel and keep an eye on it and the leading line (dye front) visible in the sample buffer. ...stripping and reprobing. Just make sure to go from least to most abundant protein if you’re stripping, since...

  18. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...conference If there’s a cool new method that you learned about at the talk, bring it to your next lab meeting...fruitful. After the conference Bring back what you’ve learned from the poster session to your lab. Perhaps some...scientific communication, and scientific networking Learn how to set up and informational interview and Expand...

  19. Pooled Library Amplifications

    Type
    Blog Post
    ...pooled library with Addgene, you may be surprised to learn that we ask for an amplification protocol with your... repeated amplifications of pooled libraries can lead to issues such as recombination and loss of plasmid...Barcode Watermelon Library 5,000,000 30 bp At least 200 ng Liquid Electroporation Protocol (DOCX...

  20. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... Resources on the Addgene Blog Learn about inducible promoters Learn about repressible promoters Check...the addition of post-translational modifications Learn more molecular biology in our Plasmids 101 eBook...
Showing: 661 - 680 of 1028 results