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We narrowed to 908 results for: Nes

Showing: 661 - 680 of 908 results
  1. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...GFP-tagged Atg8p or LC3: ATG8 is part of a group of genes that affect autophagy (ATG) in yeast. Atg8p (“p”...stands for protein) associates with autophagosomal membranes, so tagging it with GFP allows for tracking the...
  2. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...silence–but it was thorough and coherent.  To be honest, if my paper hadn’t been ridiculously more complex... in front of a very confused group of visiting Chinese undergrads. You get nervous and freeze, or forget...
  3. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...complete. The nanobody based RANbody platform from the Sanes Lab overcomes this limitation and allows for the...its sequence in the comments below. References Sanes, J.R., & Yamagata, M. (2018). Reporter-nanobody ...
  4. To Each HIS Own

    Type
    Blog Post
    ...acid, proteins containing two or more adjacent histidines are present in most production systems and can...can affect protein function. B, The string of histidines in the tag is typically between 6 to 9 residues...
  5. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...genomes, scientists can control expression of their genes of interest. While recombinases are powerful gene... knockout library that targets human epigenetic genes was deposited by Kivanc Birsoy. Find the library...
  6. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ... C. elegans embryo when all essential embryonic genes are individually depleted via RNA interference (...that can bind to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In ...
  7. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ..., and as we all know, a structural component of bones and teeth. It is an enzyme co-factor, a crosslinking...with other measurements, specifically fluorescent ones? Many of the available biosensors use a single GFP...
  8. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...Since polyclonal antibodies contain a variety of clones and thus the potential to bind many different sites...secondary antibodies rather than generally reactive ones. And make sure to check for any reported cross-reactivity...
  9. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...fiber optics need to be polished to significant smoothness to prevent drastic loss of efficiency between...diamond polishing paper of sequentially increasing fineness helps to maintain a consistent stream of light...
  10. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...more I realized that it cuts across multiple disciplines, which really epitomizes what the makerspace ...Caenorhabditis Genetics Center (CGC) at the University of Minnesota has declared our makerspace an educational institution...
  11. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post
    ...CRISPR plasmids categorized by year of deposit. Outlines show a representative proportion of CRISPR plasmids...of tools seen in Figure 3, indicates that the usefulness of popular CRISPR tools is both enduring and ...
  12. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...can be identified. Even if some mammalian cells lines have similar morphologies, being familiar with these...Twice a week often works for most fast-growing cell lines (such as HEK293, which multiplies every 16 hours...
  13. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...proteins both transiently or through stable cell lines. Both methods produce high protein yields if transfection...cells)  that allow for continuous expression of genes integrated into the insect cell genome. Both of ...
  14. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...that could directly impact patients. And, to be honest, I’ve never enjoyed grant-writing. The more I talked...academia, however, writing papers and grants is an inescapable and essential part of being able to continue ...
  15. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...provides the AAV replication (rep) and capsid (cap) genes. AAV replication uses the host’s polymerase, but... the pHelper plasmid which expresses adenovirus genes which help mediate AAV viral replication; and 3)...
Showing: 661 - 680 of 908 results