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We narrowed to 910 results for: Nes

Showing: 661 - 680 of 910 results

  1. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...GFP-tagged Atg8p or LC3: ATG8 is part of a group of genes that affect autophagy (ATG) in yeast. Atg8p (“p”...stands for protein) associates with autophagosomal membranes, so tagging it with GFP allows for tracking the...

  2. Summer SciComm: Preprints

    Type
    Blog Post
    ...transparency Another major benefit of preprints is their openness. Preprints can be read freely by anyone and are... work too openly too soon or simply a lack of awareness of the benefits of preprints. Some worry about...

  3. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...you cannot test them all, so how do you know which ones to try?  Read on and we will provide you with some...in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody assay...

  4. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...methods are often significantly longer than direct ones.  Even though the secondary antibody incubation ...to improve direct methods and avoid the indirect ones. Some Western blots, for example, use a primary ...

  5. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...silence–but it was thorough and coherent.  To be honest, if my paper hadn’t been ridiculously more complex... in front of a very confused group of visiting Chinese undergrads. You get nervous and freeze, or forget...

  6. To Each HIS Own

    Type
    Blog Post
    ...acid, proteins containing two or more adjacent histidines are present in most production systems and can...can affect protein function. B, The string of histidines in the tag is typically between 6 to 9 residues...

  12. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ... C. elegans embryo when all essential embryonic genes are individually depleted via RNA interference (...that can bind to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In ...

  13. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...genomes, scientists can control expression of their genes of interest. While recombinases are powerful gene... knockout library that targets human epigenetic genes was deposited by Kivanc Birsoy. Find the library...

  14. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ..., and as we all know, a structural component of bones and teeth. It is an enzyme co-factor, a crosslinking...with other measurements, specifically fluorescent ones? Many of the available biosensors use a single GFP...

  15. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...Since polyclonal antibodies contain a variety of clones and thus the potential to bind many different sites...secondary antibodies rather than generally reactive ones. And make sure to check for any reported cross-reactivity...

  16. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...fiber optics need to be polished to significant smoothness to prevent drastic loss of efficiency between...diamond polishing paper of sequentially increasing fineness helps to maintain a consistent stream of light...

  17. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...more I realized that it cuts across multiple disciplines, which really epitomizes what the makerspace ...Caenorhabditis Genetics Center (CGC) at the University of Minnesota has declared our makerspace an educational institution...

  18. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...can be identified. Even if some mammalian cells lines have similar morphologies, being familiar with these...Twice a week often works for most fast-growing cell lines (such as HEK293, which multiplies every 16 hours...

  19. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...proteins both transiently or through stable cell lines. Both methods produce high protein yields if transfection...cells)  that allow for continuous expression of genes integrated into the insect cell genome. Both of ...

  20. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...that could directly impact patients. And, to be honest, I’ve never enjoyed grant-writing. The more I talked...academia, however, writing papers and grants is an inescapable and essential part of being able to continue ...
Showing: 661 - 680 of 910 results