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We narrowed to 1,014 results for: REN;

Showing: 661 - 680 of 1014 results

  1. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...Also, keep all liquid containers closed when they aren’t in immediate use. This will prevent spills, but...could change their growth rates and morphology, rendering their continued use inadvisable for experiments...in a lab where multiple people are working on different things, this will help you avoid cross-contamination... Immunology. You can find Sana on LinkedIn.   References 1. Yin, R., et al. "Gene expression profiling...

  2. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... Let it incubate Check the bacterial strain Differences between E. coli host strains can impact plasmid...might want to include an extra wash step or use a different strain. For most applications, Addgene uses and...inadequate plasmid DNA yields. Why would volume make a difference? In the simplest of terms, more volume = more...conditions play a critical role in plasmid DNA yield. Different plasmids and strains will vary in their optimal...

  3. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ... ever found yourself bamboozled by all of the different kinds of information on our plasmid pages? Well...depositing lab, although you may have an entirely different use in mind! This section of our plasmid pages... you to use in your Materials and Methods and References sections when citing the use of your colleague...e.g. if it was derived from a plasmid made in a different lab - or one obtained from Addgene!).  It also...

  4. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ...anymore. The kit contains sequences for several different fluorescent proteins. Visualizing fluorescence...use our promoters and terminators, but then they aren’t sure how to convert their own coding sequence to...with the Athena SWAN Charter and it has really strengthened the network of female scientists. Twitter has...for those who go to work in cancer biology.  References: A Golden Gate Modular Cloning Toolbox for Plants...

  5. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...dimerized linear ssRNA strands (which can have a different genomic sequence from one another) — by various... with lentiviral replication.  Resources and References More resources on the Addgene blog Viral Vectors...popular lentiviral plasmids γ-Retrovirus Guide References Cullen, B. R. (1992). Mechanism of action of ...Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses, 13(7), 1288. https...

  6. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...alleles, or introduce a premature stop codon to render an RNA nonfunctional. In their quest to build a...are present in the gRNA spacer, as ADAR will preferentially edit an adenine if the template has a cytosine...(Abudayyeh et al., 2019). To avoid some of the inherent drawbacks for RNA editing of natural cytidine ... Valley contributed to updating this article. References Cox, David B.T., et al. “RNA editing with CRISPR-Cas13...

  7. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...known quantities of DNA. Additionally, an AAV reference, a virus with a known titer, can be used to confirm...both results would be valid. This is why the AAV reference is critical. It allows you to determine if your... PCR (ddPCR) does not require a standard or a reference, meaning saving on reagents (and time)! AAV titering...becoming a popular option for PCR experiments.  References Furuta-Hanawa, Birei, Teruhide Yamaguchi, and...

  8. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ... adaptations of this system have been made in different fields of study. In addition to the identification...purification with an anti-Cas9 antibody. These different tag systems and affinity purification schemes ...dystrophy (FSHD), a disease for which there is currently no cure (Campbell et al., 2018). Find plasmids...please also see our interview with Dr. Fujii. References Campbell, Amy E., et al. "NuRD and CAF-1-mediated...

  9. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ... applications. Read on to learn more about the current (and future) applications of lentiviral vectors...facilitates nuclear export of transcripts). A different viral envelope protein (Env), usually from VSV-G...plasmid number. For more information on the key differences between the 2nd and 3rd generation packaging ...use lentivirus in the comments section below! References 1. In vivo gene delivery and stable transduction...

  10. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...when separated (2), and that DBD and TAs from different systems could be combined effectively (3). In ...a more vigourous screen is to use a number of different bait and prey vectors. This has been shown to ...split ubiquitin system has been devised (8). References 1. Casadaban M.J., Martinez-Arias A., Shapira...Science. 1986; 231:699-704. PubMed PMID: 3080805. 3. Brent, R., and Ptashne, M. A eukaryotic transcriptional...

  11. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...endonuclease domain, indicating that Cpf1 functions differently. Since Cpf1 loci are widely distributed across...could be adapted for genome editing. The use of a different nuclease could potentially overcome some of Cas9...Figure from Kleinstiver et al., 2016. Cpf1’s strength may lie in its low off-target editing rates, determined...shown the diversity of CRISPR-based systems. References 1. Zetsche, Bernd, et al. "Cpf1 is a single RNA-guided...

  12. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    .... While these differences may seem subtle they result in a key physiological difference between lenti-... system is that it involves transfecting four different plasmids into the producer cells (two packaging...as the viral long terminal repeats (LTRs) are different. As a result, lentiviral and retroviral packaging...

  13. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... to a conference. Really. No one will starve while you are away a few days. Also, your children will enjoy... yourself, “I’m not married,” or “I don’t have children, so my life will be under control.” Think again... hard not to do this. For a family with working parent(s), the sooner your kids can make a decent meal...

  14. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...middle of proteins) and performs well in many different antibody-based applications.  IPI’s recombinant...virus 5 and their use in revealing antigenic differences between human, canine and simian isolates. J ...systems don’t always work across species due to differences in natural DNA repair pathways and spontaneous...at focal adhesions in living cells in several different contexts.   Figure 5: Left: Mechanism ...

  15. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ... the Shapiro lab at Caltech.   References and Resources References Piraner, D. I. et al. Going Deeper...produce ultrasound contrast. We (the authors) are currently working on improving and optimizing ARGs, with...straightforward to engineer various cell lines from different tissues and mammalian species to produce gas vesicles...

  16. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...the lab can be applied anywhere! References and Resources References Grant, A. (2017). Originals how non-conformists...inclusivity. Work with and value people who think differently from you and you will arrive at unique solutions...ability to innovate. (Finkelstein, 2019) One issue currently plaguing the scientific community is supply chain...

  17. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...variations of Cas13 and Cas12a, are combined with different fluorescent reporters for each enzyme. In addition...Zetsche et al., 2015 ). Cas12a however recognizes a different PAM site and generates 5’ and 3’ staggered ends...Jennifer Tsang contributed to updating this article. References Abudayyeh OO, Gootenberg JS, Konermann S, Joung... Mondini A, Nogueira ML, Isern S, Michael SF, Lorenzana I, Yozwiak NL, MacInnis BL, Bosch I, Gehrke L,...

  18. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ... Optimizing FLP FRT through smart engineering Different FLP versions – making FLP efficient The first ...efficiency to Cre (Kranz et al. 2010). These differences in recombinase efficiency are important to keep...should be visualized at the same time from two different plasmids this approach becomes problematic as ...the genOway press release for more information. References 1. Atasoy, D., Y. Aponte, H. H. Su, and S. M....

  19. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... The entry clone and destination vector carry different antibiotic resistance markers (indicated here ...STEP 1: Generate an Entry Clone There are a few different ways to generate our desired entry clone - human...interest: Find Gateway cloning plasmids here! References 1. Chee JY, Chin CF (2015) Gateway Cloning Technology... Digest Video Check Out Our Molecular Biology Reference Pages  ...

  20. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...of conversations with people who work in many different scientific fields. They also host AMA forums where...first makes Reddit a great platform for those who aren’t so great at coming up with answers off the cuff...future. The AMA was also cataloged for permanent reference by the Winnower. The only small downside to the...leaving out the term “implicit bias” which is apparently a troll trigger phrase these days). Redditors...
Showing: 661 - 680 of 1014 results