We narrowed to 761 results for: Rest

1. Plasmids 101: The Wide World of Natural Plasmids

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   ...Genes allowing bacteria to survive toxins are the clearest example of a plasmid benefiting its host. For ...

2. Antibodies 101: Stripping and Reprobing Western Blots

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   Blog Post

   ...blots are a great tool to identify a protein of interest in a complicated solution like cell lysate. But...

3. Corralling and Cornering the Goldilocks Antibody

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   ...and I am excited to be a part of it! If you are interested to get involved by submitting data, sending antibodies...

4. Hot Plasmids February 2024

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   ...protein, StayGold. Although it quickly saw wide interest, its dimeric nature can interfere with some applications...

5. Viral Vectors 101: Chemogenetics

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   ...development of therapies when expression must be restricted to specific circuits. Methods for targeting expression...

6. Fluorescent Proteins 101: Introduction to FRET

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   ...and can be used to create fusions with a gene of interest. These plasmids will be most useful for constructing...

7. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   Blog Post

   ...Gibson cloning, ligation-independent cloning, restriction enzyme, TOPO cloning, unknown, or other. ...

8. Overcoming the Challenges of Lentiviral Production

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   Blog Post

   ...titer, and successfully transducing a cell line of interest a lot can go wrong. We hope that we have provided...

9. Lab Automation at Addgene

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   ...I would recommend conferences like Lab Robotic Interest Group (LRIG). There is so much to see and so many...

10. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    ...Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible Cas9 activity? Check out these...

11. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    Blog Post

    ...complex of TadA and dCas to the genomic DNA of interest  Figure 1. When ABEs are in action they...

12. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    Blog Post

    ...You’ve done great research and made interesting discoveries. You’ve analyzed the data and generated beautiful...

13. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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    Blog Post

    ...as soon as you can. It is in your supervisor’s interest that you succeed, and if you cannot resolve a ...

14. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post

    ...Regenerative Medicine (CRM), Edinburgh. His research interests include optimizing genome-editing methods in brain...

15. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ... information about this gene or DNA element of interest. If the depositor has indicated that there is ...

16. Production of Virus in Insect Versus Mammalian Cells

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    ...plasmids — a transfer plasmid containing your gene of interest plus two packaging plasmids (three packaging plasmids...

17. Magnetic Control of Proteins: More than a Dream

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    Blog Post

    ...just a starting point for much more. If you’re interested in working on these questions or collaborating...

18. Pooled Library Amplifications

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    ...with. In either case, it can be useful to run a restriction digest of your amplified library on a gel to ...

19. 3D Printing Meets CRISPR Cas9

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    Blog Post

    ...undergo as it interrogates your genome. If you are interested in experiencing Cas9 up close and personal, visit...

20. Fluorescent Proteins 101: Fluorescent Protein Timers

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    Blog Post

    ...new cells are better at producing a compound of interest, thus allowing them to optimize the compound production...