Hot Plasmids: Winter 2025
Type
Blog Post
...plasmids here!
Vu, T. V., Nguyen, N. T., Kim, J., Song, Y. J., Nguyen, T. H., & Kim, J.-Y. (2024). Optimized...
We narrowed to 733 results for: SON
-
-
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post...Chan Zuckerberg Biohub Manuel Leonetti Ryan Leenay Jason Li Max Haeussler Luca Pinello Kendell Clement Andy... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...Li M, Zhou F, Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post...droplets is determined and then analyzed using Poisson statistics to determine the concentration of the... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...forward the next year when both the Yamanaka and Thomson labs showed the successful generation of human ... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog Post... efforts for over 50 years, prefers ligation of Watson-Crick base pair substrates, it demonstrates significant... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...cloning. Taking a page from bacterial cloning, we reasoned that we should be able to keep cells moist and... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post...Y or fourth chromosomes, you are a very unlucky person. Animals are diploid except for their germ cells... -
Academic vs. Industry Postdocs
TypeBlog Post...is not always the case. There are “difficult personalities” in every work setting and no manager will ever... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog Post...Cancer – a term familiar to almost every person regardless of profession, race, or gender. Almost no ... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...of the empty plasmid backbone or a researcher’s personal observations. If you are experiencing low yields... -
Genome-wide Screening Using CRISPR
TypeBlog Post...Resources Page. References McDade JR, Waxmonsky NC, Swanson LE, Fan M (2016) Practical Considerations for Using... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog Post...accidental escape to a negligible level. One major reason we chose to publish the concept and likely capabilities... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post...talking to people and representing yourself as a real person, so that students see academics as well-rounded... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post... Jason Niehaus... -
Which Fluorescent Protein Should I Use?
TypeBlog Post...effect called FRET (fluorescence [or Förster] resonance energy transfer). FRET occurs when energy transfer... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post...lines in B-D represent the pure DNA spectra for comparison. Each of these contaminants will have a slightly... -
Great Results Start with Great Standard Curves
TypeBlog Post... ELISAs and When to Use Them A Control for All Seasons More resources on addgene.org Addgene's Antibody... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post...finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator where... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...Medical Center. You’ve probably heard of Forster Resonance Energy Transfer (FRET). Through the non-radiative...