We narrowed to 1,052 results for: STI

...specific cell type. Find some transduction characteristics of select AAV serotypes in Table 2 from the...set of neurons can then be targeted with optical stimuli. The restricted light exposure to a specific area...opsin expression in their cell bodies. Optical stimulation in a different brain area can then reveal the...

...scientists around the world to conduct robust investigations into the human transcriptome. The MORF library...June, we announced a new partnership with the Institute for Protein Innovation (IPI), in which Addgene...new content, a new look, and a reorganization of existing content to make it more accessible. Other accessibility...

...With many interesting articles coming out in a myriad of journals every week, it can sometimes be difficult...We do this by promoting citation, supporting sophisticated search, and curating plasmid collections. Addgene's...whether the plasmid has been used in your system. Sophisticated search You can search for specific plasmids ...

... change in response to the cell cycle or other stimuli.   To create a colorful Cas9 toolbox, Ma et al....study is the method’s sensitivity. The authors estimate that a minimum of 150-200 fluorescent protein ...epigenetics and cellular reactions to external stimuli. It could also have major applications to cancer...

...fluorescent proteins, iRFP670 and iRFP720, as photoacoustic contrast agents for two-color imaging in animals...Verkhusha says they are always looking to improve upon existing genetically-encoded probes by making them brighter...: 10.1038/ncomms3153. PubMed) Multicontrast photoacoustic in vivo imaging using near-infrared fluorescent...

..., or as Caitlin put it, “I get a little over-enthusiastic when it comes to the labs.” While her love ... design good lesson plans, how to design test questions, and how to design lectures. I really built a ...East Coast. When we first moved out here, I was still editing from home and looking for a job.” But Caitlin...

...The discovery of GeoCas9 started with an interesting question: why engineer a new Cas9 when you can just... GeoCas9 RNPs to be incubated at high temps but still maintain activity following heat treatment. The ...

...certification on social media data, and I was given a fantastic spreadsheet template to build out a report on....be useful and how to organize the metrics in a digestible manner.  At first, the numbers I presented at...smoothly and was followed with great discussion and questions. I achieved my goal of helping everyone understand...

...endogenous function of the gene as a result but will still allow for expression pattern monitoring. Enhancer...require the trapping of endogenous enhancers to stimulate the expression of the reporter. The system is ...insertion into an exon, like promoter traps, it will still likely disrupt the gene it monitors due to transcriptional...

...conditions, cells must be able to detect extracellular stimuli. One way they do so is through G protein-coupled...of the Rluc8 insertion within the Gα. Despite exhaustive refinement, it was not possible to generate an...a target of many pharmaceutical drugs, there is still only a partial understanding of how GPCRs signal...

...manipulate gene expression in target cells. Characteristics such as flexibility, versatility, safety, and...and straightforward as possible. If you have a question about a specific plasmid element that you would...would like answered or any topic suggestions for our Plasmids 101 series, please let us know in the comments...

... process was to embed personality in pieces of plastic, but it’s gotten easier now that I identify with...the main character is much more than a piece of plastic for us now). With the personality in place, everything...children who have no preconceived obstacles, optimistically grasp at limitless potential, and who sometimes...

...Microbiology (ASM) Scientific Writing and Publishing Institute. Writing is the cornerstone of any scientist’s...and the ASM Scientific Writing and Publishing Institute (SWPI) is an extremely beneficial guide to becoming...and results to make the final product with an interesting introduction and concise discussion. Many thanks...

...content that scientists will find useful and interesting than that will simply get people ordering plasmids...analytical thinking, time management, information distillation, etc) are transferable to many different fields...thinking about preparing yourself for a career while still in graduate school, you’ll be well suited to follow...

...feedback in formal sessions using a specific list of questions after a task or period of work. Using this standard...For a formalized feedback session, answer the questions below together and discuss openly. Both parties...helped if the feedback session is over and they still don’t know how you actually feel about their performance...

...localize opsins to the soma to avoid off target photostimulation of axons and dendrites on neighboring neurons... AAV in smaller aliquots of 20 ul for serotype testing to help you pick the best AAV for your experiment...control plasmids in new serotypes. See our Serotype Testing AAV inventory for the entire collection! Plasmid...

...addgene.org to learn more or get started.  If you have suggestions about how to make the resource better or ideas...collection, please let us know by filling out this suggestion form. As we build the collection, we hope that... represent the official views of the National Institutes of Health....

...Alejandro Balazs, a Group Leader at the Ragon Institute of MGH, MIT, and Harvard, and his lab found that...SARS-CoV. These two viruses are quite genetically distinct from SARS-CoV-2 and its variants. Yet, the B.1.351... Cele S, Gazy I, Jackson L, Hwa S-H, Tegally H, Lustig G, Giandhari J, Pillay S, Wilkinson E, Naidoo Y...

...of the Ocular Genomics Institute, and an Associate Member at the Broad Institute of Harvard and MIT. ... of Oncology Target Discovery at the Novartis Institutes for Biomedical Research. Drug development was...

...nucleic acids? Sounds sort of dangerous at first suggestion! The good news is that viruses are very valuable...long-term? Expressed short-term? Answering these questions will help guide you to the right method for your...are non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform...