We narrowed to 729 results for: STO

1. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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   Blog Post

   ...) to name two more. Likewise, there are several store-bought cloning kits available which are all based...

2. How to Negotiate a Successful Lab Start Up Budget

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   Blog Post

   ...laboratory research management consultant and coach in Boston, MA. He specializes in assisting research scientists...

3. Is this the right place for me? 8 tactics for choosing a lab

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   Blog Post

   ...Symposium and #MeTooSTEM, academic bullies are hard to stop—they often have no boss or management structure ...

4. Exploring Applications of the Bioluminescent HiBiT Tag

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   Blog Post

   ...protein activity. Bioluminescence detection is a cornerstone of modern cell-based assays and enables ultra-sensitive...

5. Viral Vectors 101: AAV Variables That Matter

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   Blog Post

   ...Kügler, S. (2017). A MicroRNA124 Target Sequence Restores Astrocyte Specificity of gfaABC1D-Driven Transgene...

6. Technologies Enabled by NanoLuc® Luciferase

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   Blog Post

   ...furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp to a compact powerhouse...

7. Generating Mouse Models Using CRISPR/Cas9

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   Blog Post

   ...Humanization (25-kbp) Created Using Embryonic Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches. Sci Rep...

8. PiggyBac-ing Through the Genome Editing Field

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   Blog Post

   ...re-cutting. Transposase removal of the cassette later restores the target sequence. This allows researchers to...

9. Plasmids 101: Restriction Cloning

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   Blog Post

   ...to covalently link the backbone and insert and, PRESTO, you have a full plasmid ready to be used in your...

10. Molecular Biology Reference

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    Guide

    ...bacterial cells that have been preserved as glycerol stocks. Function in many species — Plasmids can drive ...inoculating bacterial cultures . Antibiotic Recommended Stock Concentration Recommended Working Concentration ... acid. There are 20 amino acids plus a start and stop codon. Below you will find helpful resource tables..., UAC Valine Val V GUU, GUC, GUA, GUG Start AUG* Stop UAG (amber), UGA (opal), UAA (ochre) *AUG is the...

11. Finding Your Science Policy Path

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    Blog Post

    ...move into science policy. Read broadly about the historical roots of science policy to connect to your why...

12. Deep Dive: Statistical Tests (Comparisons)

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    Blog Post

    ...distribution you are working with, graphing data with a histogram is sometimes sufficient to determine normality...

13. Sequencing Options for CRISPR Genotyping

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    Blog Post

    ...Targeted amplicon sequencing doesn’t tell the whole story Figure 1: Schematic representations of the...

14. Antibodies 101: Flow Cytometry

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    Blog Post

    ... then gate the B cell population and create a histogram of CD19-containing cells versus total cell number...

15. Which Fluorescence Microscopy Technique is Best for Me?

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    Blog Post

    ...Illumination (SIM), Single Molecule Localization (PALM/STORM), or Reversible Saturable Optical Linear Fluorescence...

16. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post

    ...experiments. Immune response dynamics are much better understood thanks to these technologies but many questions...

17. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post

    ...., Butler, R., Bewersdorf, J., Rothman, J. E., Johnston, D. S., Schepartz, A., & Toomre, D. (2019). Labeling...

18. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post

    ...and for good reason. For the first time in the history of neuroscience research we have a technology that...

19. Molecular Cloning Techniques

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    Guide

    ...their recognition site allows for the creation of custom overhangs, which is not possible with traditional...have utilized this approach to create compatible custom overhangs that can then be efficiently assembled...yeast the fragments of DNA to be fused along with custom synthesized DNA oligos that span each junction....

20. Gamma-Retroviral Vector Guide

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    Guide

    ...VSV-G due to wide infectivity VSV-G (vesicular stomatitis virus G protein) is the most common envelope ...supernatant containing the viral particles is removed and stored or centrifuged to concentrate. Crude or concentrated... The viral envelope gene; typically vesicular stomatitis virus G glycoprotein (VSV-G), an envelope protein...