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We narrowed to 939 results for: TIM

Showing: 661 - 680 of 939 results

  1. Working with Nuclear Receptors

    Type
    Blog Post
    ...be required by one or more NRs for efficient and timely activation or repression of gene expression (see...the research literature and acquiring them in a timely manner. Addgene represents an excellent solution...

  2. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...for targeting thousands of long ncRNAs at the same time (Liu et al. 2018). However, removing large lncRNA... the ncRNA (Figure 1D). While this method can be time-consuming, it is relatively effective in reducing...

  6. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...underdressed, but read emails from HR carefully because sometimes companies have relaxed dress codes for interviews...interview is complete. Remember, your interviewers took time out of their day to talk with you, so be engaged...

  7. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...not only highlighted new tools but also included time for questions and a group discussion on the best... to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In 2018, the lab...

  8. Engaging with science and society at pgEd

    Type
    Blog Post
    ...for a year, I’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a...courses in social, health or policy-related topics, as time permits. Also seek out opportunities to communicate...

  10. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...companies and start-ups choose to locate there. I made time to go into the city of Guangzhou and see some of...surprising” small RNAs can be and posited that “we underestimate living things.” He further cautioned the distinction...

  12. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...the mutants, analyse their motor behaviour, and ultimately submit our new strains back to the CGC for other...differs from that of wild cats with the goal of optimizing the health of domesticated cats and learning ...

  13. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...be distinguished using conventional flow can (sometimes) be distinguished using spectral flow since the...signal, even for rare cellular markers, beads can sometimes distort the fluorescent signature upon antibody...

  17. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...we have analyzed hundreds of viral vectors and estimate that recombinase-independent recombination occurs...that users titrate their AAV vectors to find the optimal dose that allows for sufficient transgene expression...

  18. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...recombination, but with longer homology arms comes more time spent cloning. In contrast, the short homology required...the risk of off-target effects, Sakuma et al. optimized the PITCh-gRNA for minimal off-target binding ...

  19. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...acquire one image, thereby significantly reducing the time it takes to image through a large z-stack (8).  ...compatible with LSFM, a method termed COLM (clarity-optimized light-sheet microscopy) (16).  Both 3DISCO and...

  20. MXS Chaining

    Type
    Blog Post
    ...cloning site (MCS). Modules are assembled one at a time by standard restriction enzyme digestion followed...DNA sequences, no restriction enzymes needed Not optimal for joining sequences with a high degree of identity...
Showing: 661 - 680 of 939 results