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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,082 results for: TOR;

Showing: 661 - 680 of 1082 results

  1. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...great deal more information that one might like to store about FPs than is currently represented at fpvis...a centralized, community-editable resource for storage and dissemination of information about FPs. FPbase.org...elaborate spectrum analysis tools, and a better way to store and represent within-study photobleaching comparisons...

  2. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...interactions and growth within the biofilms will then be monitored using fluorescence microscopy and FACS. In an ...to create gene deletions of important unknown regulatory regions in Pseudomonas and Klebsiella spp. The...projects to reach audiences beyond our plasmid depositors and requesters. Check out some of our recent ...

  3. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...how I got my current job as a Communications Coordinator. Wondering how you can develop more transferable...Theresa Liao (@theresaliao) is the Communications Coordinator for the Department of Physics & Astronomy, University...Experimental Medicine. She is the Community Relations Director for Curiosity Collider (hyperlink https://curiositycollider.org...

  4. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...Excellent scientists learn from advisers and other mentors by collaborating as they craft sentences for abstracts...in all STEM fields become excellent science communicators. To empower graduate students to become ambassadors...careers in science communication The strength of story telling Science communication: 9 strategies to get...

  5. Antibodies 101: Conjugation

    Type
    Blog Post
    ...way they are, the research antibodies in your refrigerator often need a little help to be useful. Mainly...of e-Amino Groups Available for Conjugation of Effector Molecules to Monoclonal Antibodies. Hybridoma ...resources on the Addgene blog Antibodies 101: Buffers, Storage, and ConjugatesAntibodies 101: The Basics of Western...

  6. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...interview better! As a scientist turned science communicator, I’ve done a lot of public speaking, and I'm...six figures. Averaging ten panels each. (For this story, it is important to know that the student presenting... turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving board” so you end...

  7. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...of emitted photons before they are destroyed. Factors that affect photostability The photobleaching rate...fluorophore will bleach. This is, however, not the full story. The photobleaching rate does not depend on excitation... for each of these conditions. On top of these factors, environmental conditions such as cellular redox...

  8. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...one to study RNAs expressed from their native regulatory elements. Controls for RaPID experiments RaPID...Ramanathan et al. also used the Contamination Repository for Affinity Purification (CRAPome), to identify...RNA-binding protein that’s highly expressed in neural progenitor cells. Knock-down of QKI decreased Zika viral...

  9. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...team and software engineers to update our Lab Inventory Management System (LIMS) to accommodate our new... discussed them in meetings and 2) creating a pictorial guide for our engineers (Fig. 2). Creating this... a lot on context. New people in the lab or collaborators will not have the context you do, which means...

  10. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...is correct. It's also best to use 1-2 ug of the vector for digestion. PCR Design primers that overlap ... probably left your plate for too long in the incubator. The small colonies are called satellite colonies...after the screening, sequence the plasmid. Many vectors contain sequences that are complementary to standard...

  11. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...inspiration to resolve the technical hurtle of monitoring protein turnover of specific proteins in situ...Erin M Schuman, a former Caltech researcher, now Director at the Max Planck Institute for Brain Research...to all audiences. Right now, she is a science instructor at The Innovation Institute in Boston. When Eugenia...

  12. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse protein ...GBPs together to create a complete transcription factor, activating luciferase transcription. Tang et al...delivered throughout the nervous system, including the motor cortex, cerebellum, and spinal cord. Cre-DOG is ...

  13. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...aligns with your work, your advisor may want to mentor you during the grant application process. When ...first. Additionally, reach out to peers and collaborators who have succeeded at grant writing to see what...write your first grant proposal" by Soapbox Science Editor in Nature Blogs "How to write a research project...

  14. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...They play important roles in many cellular and regulatory processes, but much of their functionality is...effects can be minimized by disrupting lncRNA regulatory elements that are crucial for transcription and..., on the other hand, recruits transcriptional activators such as VP64, SAM, or SunTag to specific ncRNA...

  15. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...NG  - SpRY nuclease  C to T Base Editor SpRY A to G Base Editor SpRY   NYN/NRN NYN is less effective...Engineering a CRISPR Variant with PAM Flexibility History of CRISPR Cas – A Tale of Survival and Evolution...

  16. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...Daniel M. Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some ...particular tool has gained widespread popularity – and notoriety – as a tool of choice in the field: dCas9 fused...Daniel M. Sapozhnikov is a graduate student in the laboratory of Dr. Moshe Szyf at McGill University, working...

  17. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...improved search algorithm which was trained on key factors from past PubMed user searches, such as clicks ...other areas of science. Scientists often post tweetorials, or threaded summaries, of their papers. These...publications in the fields of research they support. Monitoring these types of accounts can help you find papers...

  18. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...2019; Chen & Liu, 2023). This method uses a prime editor (typically a Cas9 H840A nickase fused to a reverse...optimization efforts. This holds true for any prime editor experimentalist, and especially shines once large-scale...Collection The CRISPR Guide Empty gRNA Expression Vectors...

  19. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...If you have ever worked in a molecular biology laboratory you have likely done a polymerase chain reaction...method that can help save time and effort in the laboratory.  There are two main categories of multiplex ... Variations Quantifying low abundance targets Monitoring subtle changes in target DNA levels Multiplex...

  20. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... like PCR, cloning, transfection, or long-term storage. But what about those pesky situations where your...maximize cell density and plasmid yield as these factors are generally, but not perfectly, correlated. ...manufacturer. On Addgene plasmid pages, you may see depositor recommendations to grow a plasmid at 30℃ or room...
Showing: 661 - 680 of 1082 results