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  2. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...ll need to produce your viruses in-house.   Introducing the systems There are many ways that viruses ...insectspodoptera frugiperda (lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077...

  3. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...protein’s structure into experimentally determined electron density, have been known to say something silly...educators. Using these hands-on tools, educators introduce their students to this otherwise invisible molecular...

  4. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...used to tag endogenous genes and simultaneously introduce a selection marker for screening modified strains...with a Cas9 expression vector. They can also be introduced directly into a Drosophila S2 cell line stably...

  6. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...not that I would know about this… A no template control (NTC) should always be included. While the physical...recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols in molecular ...

  9. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...image acquisition is performed using cameras with electron multiplying couple-charged device (EMCCD) or complementary...use of viruses allows much better spatiotemporal control over labeling methods as compared to transgenic...

  10. DNA Purification Without a Kit

    Type
    Blog Post
    ...but the addition of a high concentration of a chaotropic salt (guanidine hydrochloride for the plasmid... glass milk, gel slices are first placed in a chaotropic salt sodium iodine (NaI) solution. NaI has two...

  12. Tips for arabidopsis transformation

    Type
    Blog Post
    ...like to isolate a minimum of five. You have no control over how many insertions occurred. Having too many...segregate 3:1 in the T2 generation. You also have no control over where your construct will be inserted. If ...

  15. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ... easiest to adapt to genome editing, but the introduction of type V Cpf1-driven systems has added another... endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due to its proximity to the cleavage...

  17. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...that are more career-focused. After these talks, introduce yourself to the speaker and offer your business...them for work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great...

  19. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...defective chloramphenicol resistance gene (CamR) by introducing a point mutation (H193Y). The reversion of this...promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple...

  20. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...world and corrective approaches and appropriate controls are required to make this method useful for dynamic...measurements. Extra care should be taken so as not to destroy the donor molecule. Fluorescence lifetime imaging...
Showing: 661 - 680 of 914 results