We narrowed to 911 results for: Tro;

...Importantly, all of these assays are performed in vitro. Assays to detect FP dimerization in in vivo cellular...Structural Biology. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

...image acquisition is performed using cameras with electron multiplying couple-charged device (EMCCD) or complementary...use of viruses allows much better spatiotemporal control over labeling methods as compared to transgenic...

...False, embed_button=False, autoplay=False, hidden_controls=False, loop=False, muted=False, width='600'... impacted. The inferred weekend effect is, in retrospect, generally more dramatic than the COVID-19 effect...

...but the addition of a high concentration of a chaotropic salt (guanidine hydrochloride for the plasmid... glass milk, gel slices are first placed in a chaotropic salt sodium iodine (NaI) solution. NaI has two...

...like to isolate a minimum of five. You have no control over how many insertions occurred. Having too many...segregate 3:1 in the T2 generation. You also have no control over where your construct will be inserted. If ...

...anyone can find enhancers with Quilt. Seek and destroy enhancers with CRISPR Armed with the genome math...ESCs) with a lentiviral vector, an attenuated retrovirus in the same family as HIV. By design, our lentivirii...

...can occur in two different orientations, but is strongly biased towards T-RL, where the R end is proximal...coli (Klompe et al., 2019). Recently, the lab introduced a streamlined expression approach (Fig. 2A), ...

... easiest to adapt to genome editing, but the introduction of type V Cpf1-driven systems has added another... endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due to its proximity to the cleavage...

...glycerol stocks, prepped DNA ready for our quality control processes - all of these samples have the 2D barcodes...along the way involves good aseptic technique and controls to ensure that nothing is contaminated. We use...

...years of experience in my job, I was willing to introduce myself to someone new and ask for help. That was...confidence to meet someone new, as they’d been introduced to several people by their mentor, as well as...

...that are more career-focused. After these talks, introduce yourself to the speaker and offer your business...them for work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great...

...defective chloramphenicol resistance gene (CamR) by introducing a point mutation (H193Y). The reversion of this...promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple...

...world and corrective approaches and appropriate controls are required to make this method useful for dynamic...measurements. Extra care should be taken so as not to destroy the donor molecule. Fluorescence lifetime imaging...

...alongside standard surface labels. Here, I will introduce you to a few useful labeling and staining techniques...Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...

...If you read my eBook and it helps you, please introduce yourself to me by email or in person. If you feel...Medical Institute. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

...evident that widespread testing is crucial in controlling this pandemic. Currently, the CDC uses RT-qPCR... need for thermocyclers. This is followed by in vitro transcription and Cas13 is then used to detect the...

...Like graphing data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist...women), visibility (how to get it, especially if introverted ), and networking (how to get people to remember...

...exonuclease feature of T4 DNA polymerase, which must be controlled by the presence or absence of dNTPs. Secondly...assembly a ligase is added to repair the nicks in vitro, whereas in SLIC these constructs are repaired in...

...Epigenetic modifications are an additional layer of control over gene expression that go beyond genomic sequence...catalytically dead modifiers are also available as controls. For an up-to-date list of CRISPR epigenetic tools...

...Structure-guided directed evolution If you desire a bit more control, structure guided directed evolution might be more...evolution, all while maintaining a higher degree of control and efficiency! Screening mutant libraries After...