We narrowed to 953 results for: cha;

1. London Calling - Addgene’s New Europe Office in London

   Type

   Blog Post

   ...States - Cambridge, MA to be exact. Today, that changes. Today, Addgene launches a brand new Europe office...scientists together from all over the world to exchange research materials in an easy way. She has a passion...

2. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

   Type

   Blog Post

   ... interest by taking advantage of bacterial characteristics such as prototrophy, the ability to synthetize...used for negative selection on 5% sucrose plates. Exchange of the sacB gene for the gene of interest allows...

3. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

   Type

   Blog Post

   ... Mary, Max, Maya, Meghan, Melina, Meron, Michael Lo., Michael Le., Michel, Michelle, Morgan, Natashia,...

4. SunTag and Fluorescent Imaging

   Type

   Blog Post

   ...localized, and how its localization and expression may change across various conditions. However, this system...Improved brightness and signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified...

5. Viral Vectors 101: Parts of the AAV Packaging Plasmid

   Type

   Blog Post

   ...Introducing changes and/or short peptides sequences into these variable loops is one method for changing a capsid...

6. New Viral Vectors - Fall 2024

   Type

   Blog Post

   ...types of the basal ganglia. They work via newly-characterized enhancers that enable a whole host of (awesome...AAV-GfaABC1D-Cre-4x6T AAV5 Recombinases Stanley Thomas Carmichael New viral prep pAAV-EF1a-fDIO-Cre AAV5 Recombinases...

7. CRISPR 101: Drug discovery and CRISPR

   Type

   Blog Post

   ...model can be generated, and drugs screened for a change in pathology. If the disease pathology is complex...Target identification Not all diseases are fully characterized or have a simple pathology. In these cases, ...

8. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

   Type

   Blog Post

   ...growth is possible even if your job title doesn’t change. Master’s level training can prepare you to present... career with a master’s. While it's possible to change fields of research with a PhD, a master’s is less...

9. Oh, The Places You Can Go: Careers in Science Communication - Product Development

   Type

   Blog Post

   ... a job and I stumbled upon eScience by luck, by chance, by complete happenstance. It was on Indeed or ...company. And make sure to subscribe to the Career Channel of the blog so you don’t miss the future posts ...

10. Sleeping Beauty Awakens for Genome Engineering

    Type

    Blog Post

    ...cells, but they are susceptible to cells’ defense mechanisms. The use of non-viral vectors, like transposons...sequences and then testing various combinations of changes. The basic functional version of the Sleeping Beauty...

11. 10 Ways to Share Your Science!

    Type

    Blog Post

    ...people like a good story and science involves characters – in this case you – on a quest to ask and answer...   Consider participating in a future SciFund Challenge. While the goal is ostensibly to get some funding...

12. Advice for Choosing a Research Project

    Type

    Blog Post

    ... apart a piece that can address a topic in a mechanistic way using the technology available.” Choosing...combining “new discoveries or technologies increase chances that your project will be fresh as well.” Don't...

13. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type

    Blog Post

    ...LOV2 and Zdk1 to dissociate due to conformational changes, and as a consequence, EB1 protein function is ...expansion of the genome editing toolkit. However, a challenge in optimizing base editing technologies across...

14. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type

    Blog Post

    ...antibody fragments typically engineered from heavy-chain only antibodies found in camelids. They possess ...H2O2, APEX2 labels electron-rich amino acid side chains (e.g. Tyrosine) with biotin. These biotin-labeled...

15. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

    Type

    Blog Post

    ...more. Downloadable materials are available free-of-charge from the ABRC Outreach website. Seeds, which are...system comes with fewer ethical and logistical challenges than does working with animals. Because Arabidopsis...

16. The Strength of Story Telling

    Type

    Blog Post

    ...anecdotal forms of information — identifying with a character, building emotional investment and following cause-and-effect... to date, invite people to ask questions, be approachable. Engage with your audience and try to learn ...

17. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type

    Blog Post

    ...antigen-binding fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs) (Inbar et al., 1972) ...within the variable portions of heavy and light chains. Proceedings of the National Academy of Sciences...

18. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type

    Blog Post

    ...general comments and adjust them if my opinion changed after this more detailed look.        Encourage... Tester, where she focuses on (1) salt-induced changes in root-to-shoot ratio in Arabidopsis, (2) expression...

19. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type

    Blog Post

    ...particles containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B,...Redon R, Moullier P, Léger A (2015) Advanced Characterization of DNA Molecules in rAAV Vector Preparations...

20. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type

    Blog Post

    ...our growing group of Addgene AAV data sharing champions? If you’ve recently used Addgene AAV tools, we...caption. For microscopy images, please include channel imaged, a color legend for probes/dyes/labels, ...