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  1. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...visiting role is because the host lab or institute provides something that you can’t obtain at your current...skills and equipment! Preparing for your visit   Design and communicate a research plan Your time at the...

  2. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...biomass production techniques in the following decades, Phillips made the decision to release the K. phaffii...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...

  3. Hot Plasmids - January 2023

    Type
    Blog Post
    ...effects, both of happen with Cas13. CRISPR-Csm degrades both nuclear and cytoplasmic mRNA with 90-99% ...crystalizing the hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you...

  4. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...best serotype for their particular study, which includes comparing the transduction patterns of different...fluorescence of the sensor. This “contrast” variant provides improved detection for tracking large populations...

  5. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ... chromosome-specific loci in the genome and the guides are relatively unstable.   Figure 1: CRISPRainbow... the gRNA tetraloop and went through subsequent design iterations to increase stability and minimize misfolding...

  7. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...mammalian plasmid components, it may be useful to describe the means of introducing genetic material (such...the plasmid in the same way. Selection methods, described below, are typically employed when creating stable...

  8. AAV Viral Preps

    Type
    Collection
    ...comprehensive high-quality quality control process includes titering, purity assessment, sterility testing...

  11. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...induction of a promoter. Below, you’ll find a short description of these three types of inducible promoters, ...yellow flame light-regulated two component systems designed by the Tabor lab. Which inducible promoter is ...

  12. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...control, generates  educational materials, and provides viral vector technical service (details about ...tracking. Our Software and IT Teams made major upgrades to our inventory management systems to enable ...

  13. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...1x1012 vg/mouse is more standard) and reflects a desire for very high MOI on top of very high coverage....researchers have used the flexibility of AAV genome design in remarkably creative ways. The continued development...

  14. Antibodies 101: Isotypes

    Type
    Blog Post
    ...molecules are recruited by the antibody to help destroy and remove a pathogen. Different isotypes also ...well as the genes that encode those proteins are designated by the lower-case Greek letters μ, δ, γ, ε, and...

  15. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ... such as citizenship status, academic year, and grades that serve as automated filters of consideration...and cover letter revised by your network, which includes your friends, family members, and professional...

  16. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...promoter should flank this component as well. On both sides of this promoter-gene combo are two long terminal...Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from: https://www.ncbi.nlm.nih.gov/books...

  17. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...through a seminal paper from Peter Schultz’s lab describing a technique that allowed for intentional incorporation...reagents accessible to your lab. The solution GCE provides almost endless possibilities for researchers, ...

  18. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a ...delivery of the actual virus to which immunity is desired. Instead, components of the virus are now delivered...

  19. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...target cells and organisms, and when that cargo is designed to make you sick…well, you feel it. But the same...host genome or temporarily express whatever it encodes. This ability to infect cells with a high efficiency...
Showing: 661 - 680 of 983 results