We narrowed to 986 results for: des
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TypeBlog Post...through a seminal paper from Peter Schultz’s lab describing a technique that allowed for intentional incorporation...reagents accessible to your lab. The solution GCE provides almost endless possibilities for researchers, ...
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Viral Vectors 101: Viral Applications
TypeBlog Post...biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a ...delivery of the actual virus to which immunity is desired. Instead, components of the virus are now delivered... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...target cells and organisms, and when that cargo is designed to make you sick…well, you feel it. But the same...host genome or temporarily express whatever it encodes. This ability to infect cells with a high efficiency... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog Post... such as citizenship status, academic year, and grades that serve as automated filters of consideration...and cover letter revised by your network, which includes your friends, family members, and professional... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post...mentoring questions I set out to answer. First, I will describe some of the many ways you can approach finding...Watch for my upcoming mentoring post in which I describe how to organize a successful peer mentoring circle... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...Solution: In Zhou et al, the Lin lab present a generic design for creating photoswitchable kinases and use MEK1...create other photoswitchable kinases? The general design of psMEK1 also worked for creating three other ... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...Amsterdam. The previous two posts in this series described a practical approach to selecting a bright fluorescent...preprint, or documenting it in some other way. Besides the data, new plasmids and/or cells or organisms... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog Post...of copy number (Segall-Shapiro et al., 2018). Designing stabilized promoters To keep gene expression stable...ideally be optimized to promote generation of the desired product while avoiding the accumulation of toxic... -
RANbodies: Reporter Nanobody Fusions
TypeBlog Post...overcomes this limitation and allows for the flexible design and small scale production of antibodies. Problems...Their His tags allow for further purification, if desired, but it's not required for their use. Each RANbody... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...electrical activity in the brain by inserting electrodes into the brain. But this process is time consuming... the resulting optogenetic plasmid tools which includes plasmids encoding a fusion between cPYP and tgRFP... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post...ensure scientists everywhere can access plasmids despite the evolving export/import rules. Our shipping ...most popular website in the world. I know, right? Upgrades to the internal LIMS pages and excellent work ... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post...and Accelerate Research Discoveries) Challenge provides grants for rare disease research. This year is...mutations in one of these parts, the GNB1 gene, which codes for G𝛃1 subunits of G protein complexes, lead to... -
Addgene: The First Twenty Years
TypeBlog Post...business practices, like accepting e-signatures and designing a website users could easily navigate. This meant...Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, responding to a request... -
Summer SciComm Series: Cognitive Load
TypeBlog Post... other sources. Mode of communication Different modes of communication take up different amounts of mental...Maybe I needed to employ a visual aid instead of describing something verbally. Sometimes a group was just... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...sulfurtransferase (MPST) which catalyzes the desulfuration of 3-mercaptopyruvate and generates an enzyme-bound...transferred to thiol-containing acceptors. These hydropersulfides modulate protein activity and have important... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post...in one direction. Cas3 is thus not helpful for designer genome edits, but it is effective at wiping out...David, Chen, S., Janice, et. al. Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. Science, 362... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...showing the potential for broad use. This kit is designed for use with acceptor vectors from the MoClo Toolkit...tracing Article contributed by Erin Sanders CreLite, designed by the Eisenhoffer lab, is a new tool that increases... -
Plasmids 101: Cre-lox
TypeBlog Post...using a resistance marker; however, it is often desirable to remove the marker after the initial selection... Combining this with some of the loxP methods described above, a genetic modification can be restricted... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post... Guardian, BBC Breakfast or the Londonist. BBC provides a peak into their day-to-day business with popular...Co-Ordinating Centre for Public Engagement (NCCPE) provides on-site courses in public engagement for institutions... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post... but educators face significant challenges in designing suitable projects. In the O'Connor lab, we recognized...Over Time project The Pathways over Time project provides a framework for these undergraduate research questions...