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Showing: 661 - 680 of 960 results

  1. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...While various Cas9 mutants have been engineered to perform unique editing tasks, classic Cas9 nuclease, which...generating a monoclonal Cas9-expression line prior to performing a screen or even a single gene knockout. Top ...

  2. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...different doses. See Figure 4 in this paper for more information.   Q6 & Q7: We have been attempting to transduce...high coverage. Please see this paper for more information about PHP.eB brain labeling at various doses....

  3. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...survive this step. Then, a limiting dilution is performed to isolate individual hybridomas and ELISA is ...Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:258–268. https://doi.org...

  4. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...quite different from gamma-retrovirus, but they perform very similar functions to infiltrate the host cell...and mediate efficient viral replication. More information on these viral genes is available in the adenovirus...

  6. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...products for packaging (Gag, Pol, and Env), the isoforms of these proteins, as well as the viral LTRs, ...different viruses and thus use slightly different isoforms of these packaging components. Therefore, lentiviruses...

  7. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...How can I tell if my enzyme will cut? Whether performing a digest for cloning purposes or for diagnostics...consult the REBASE database for more detailed information. Enzyme Dam methylation Dcm methylation EcoKI...

  10. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ... genetic system, and a highly versatile DNA transformation system.Unlike most other microorganisms, yeast...plasmids oftentimes utilize an ARS to aid in high transformation efficiency; however, this region does not necessarily...

  11. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...interactions with RaPID RaPID is similar to the BioID platform for identifying protein-protein interactions, ...proteins reported by other research groups that performed BirA*-based affinity purification mass spec experiments...

  12. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...similar rates to untreated controls. Ma et al. performed sequencing of 23 potential off-target editing ...advancement in our understanding of how CRISPR will perform in embryos but also shows that many more experiments...

  13. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...survey found that many researchers feel they lack formal training in a variety of transferable skills. At... Step One: Identify the problem and gather information to help you understand it. Step Two: Come up ...

  14. Antibodies 101: Conjugation

    Type
    Blog Post
    ...conjugated antibody will have been verified to perform the same as its unconjugated version, but if you...that you will need to confirm your antibodies’ performance after the reaction.  If you are looking to conjugate...

  15. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...the very nature of the way the experiments were performed, they did not see anything unique to male stem...reporting the sex of cells and tissues—when that information is reliable—is important to ensuring excellence...

  16. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...IIS sites and will be re-digested. In contrast, formation of the desired ligation product is irreversible...enzyme recognition sites after ligation favors formation of the construct of interest. Although efficiency...

  17. Engaging with science and society at pgEd

    Type
    Blog Post
    ...crucial if we want to make sure that everyone can be informed and empowered to make their own decisions about...social media and blog posts, lesson plans, or information briefs for policymakers. The rapidly changing...

  18. Hot Plasmids - October 2020

    Type
    Blog Post
    ...time of synthesis and, after a few hours, a conformational change causes the protein to fluoresce red....lentiviral preps in addition to the pooled library format! The library inhibits over 18,000 human genes. ...
Showing: 661 - 680 of 960 results