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  1. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ... need a high efficiency of transformation (for example, if you are working with a library or an especially...making competent cells are available online. A few examples are provided here for reference: OpenWetWare ...

  2. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...cassettes for selection of transgenic plants. The complete GreenGate toolkit, now available at Addgene, also...had been humming along at 99 percent efficiency, simply stopped working. They finally traced the problem...

  3. Reflections and Looking Ahead

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    Blog Post
    ...by adding long-read sequencing capabilities to complement our next-generation sequencing, setting new standards...Request, a service that dramatically expands and simplifies your viral vector options. Almost any AAV transfer...

  4. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

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    Blog Post
    ...provide you with that control in a modular way. For example, say you would like to label neurons with mCherry...pmSyn1-EBFP-Cre Of course there are also more complicated ways to use this particular AAV. For instance...

  5. SciComm with the Experts at Science in the News Part 2

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    Blog Post
    ... at a local bar. It doesn't have to be crazy complicated! Listen to the full podcast for more great science... a Science Communication group started. It's as simple as getting people together at a bar. Chapter 10...

  6. X-CHIME: Context Dependent Germline Knockout in Immune Cells

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    Blog Post
    ...manner, but these systems have not been widely employed in vivo. The CHIME and new X-CHIME systems, developed...containing a gRNA. These transduced stem cells can be implanted in irradiated recipients to create mice with gene...

  7. Science as Art

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    Blog Post
    ...could provide a new tool to study old and unique samples, such as those collected by Darwin on his voyage...incompatible with the need to conserve these unique samples, meaning that we know very little about these specimens...

  8. Addgene is Expanding Our Viral Vector Service!

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    Blog Post
    ...the service to be fulfilled and QC assays to be completed and reviewed. The timeline therefore will be as...follows:  Request review: 1–2 days Prep and QC completed: 6–8 weeks Average shipping time: 1–2 days (USA...

  10. What's New in CRISPR - March 2020

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    Blog Post
    ... Blue light renders the NLS accessible and the complex enters the nucleus where dCas9-Mxi1 can repress.... In the dark, the NLS is inaccessible and the complex remains in the cytoplasm. Read the paper in Cellular...

  11. Mapping the 4D nucleome with CRISPR/Cas9

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    Blog Post
    ...could also have major applications to cancer, for example, the visualization of chromosomal translocations...Addgene. A protein-tagging system for signal amplification in gene expression and fluorescence imaging....

  12. In Living Color: The Skinny on In Vivo Imaging Tools

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    Blog Post
    ...signal-to-background ratio, thus, allowing higher resolution in samples containing substantial autofluorescence background...direction of biosensors capable of revealing, for example, how muscles work inside a living animal. For those...

  13. Embracing Serendipity: A Crucial Element in the PhD Journey

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    Blog Post
    ...serendipity during your PhD studies and offer two simple steps to welcome these unforeseen moments. Widening...everything is a miracle.” This quote encourages us to contemplate our approach to life and how our viewpoints influence...

  14. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...hybridoma, etc.), as well as sex and age at time of sampling if available. STR profiling for human, mouse, ... publications cited. If the information seems incomplete or unclear, Bairoch follow up via email to the...

  15. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

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    Blog Post
    ...information.” Addgene is here to help scientists accomplish their goals. Many nonprofits must spend a lot...thought leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters...

  16. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...wrong. Whether it be a bad fridge freezing your samples, or a dysregulated water bath boiling your experiments...faced an odd situation with experiments? Our art simply mirrors our lives at this point! It was a great...

  17. Tips for Improving Your Next Manuscript

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    Blog Post
    ...spent collecting it will have been worthless. But simply writing down your data doesn’t guarantee a publication...portion of the paper will be generated. After you complete a publication quality figure, produce the figure...

  18. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...prepaid return shipping label. To return the box, simply peel your address sticker off the box, remove the...packaging (no coated tissue culture plates, for example). Check the link for more details on what they ...
Showing: 661 - 680 of 929 results