We narrowed to 938 results for: mpl
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TypeBlog Post...mck-PGC-1a1 mice found it wasn’t that they were simply immune to stress altogether. Their muscles showed...muscle and brain, which may have therapeutic implications, say Hyo Youl Moon and Henriette van Praag of...
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A Conference By Postdocs For Postdocs: Future of Research
TypeBlog Post...planning and execution. I am seeing many similar examples of science trainees taking a proactive role in... Consulting. I know of many schools that have completely student/postdoc-driven biotechnology clubs such... -
Save Time and Money by Making Your Own Competent Cells
TypeBlog Post... need a high efficiency of transformation (for example, if you are working with a library or an especially...making competent cells are available online. A few examples are provided here for reference: Untergasser ... -
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog Post...advantage of KTRs is that they are easy to create and simple to multiplex. Problems with FRET kinase reporters...swapping of fluorescent proteins and multiplexing is simple when fluorescent proteins with limited spectral... -
Science Communication for Everyone
TypeBlog Post... so on, differently than scientists would. For example, “manipulate” could mean “illicit tampering” to...founder of The Addictive Brain. Prior to this, he completed PhD from Philipps-University Marburg, Germany ... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog Post...metagenomic datasets to try to determine whether simpler and maybe smaller Cas systems could exist in nature... more primitive version of the larger and more complex Cas9 and Cas12 proteins. Cas14 cleaves ssDNA But... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog Post... of the coast of Washington state. Using these samples, he was able to isolate two proteins from the jellyfish...organisms to find the culprits. He even obtained samples of certain ones from local pet shop aquariums in... -
Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog Post...cassettes for selection of transgenic plants. The complete GreenGate toolkit, now available at Addgene, also...had been humming along at 99 percent efficiency, simply stopped working. They finally traced the problem... -
Reflections and Looking Ahead
TypeBlog Post...by adding long-read sequencing capabilities to complement our next-generation sequencing, setting new standards...Request, a service that dramatically expands and simplifies your viral vector options. Almost any AAV transfer... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog Post...provide you with that control in a modular way. For example, say you would like to label neurons with mCherry...pmSyn1-EBFP-Cre Of course there are also more complicated ways to use this particular AAV. For instance... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog Post...manner, but these systems have not been widely employed in vivo. The CHIME and new X-CHIME systems, developed...containing a gRNA. These transduced stem cells can be implanted in irradiated recipients to create mice with gene... -
SciComm with the Experts at Science in the News Part 2
TypeBlog Post... at a local bar. It doesn't have to be crazy complicated! Listen to the full podcast for more great science... a Science Communication group started. It's as simple as getting people together at a bar. Chapter 10... -
Science as Art
TypeBlog Post...could provide a new tool to study old and unique samples, such as those collected by Darwin on his voyage...incompatible with the need to conserve these unique samples, meaning that we know very little about these specimens... -
Addgene is Expanding Our Viral Vector Service!
TypeBlog Post...the service to be fulfilled and QC assays to be completed and reviewed. The timeline therefore will be as...follows: Request review: 1–2 days Prep and QC completed: 6–8 weeks Average shipping time: 1–2 days (USA... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog Post...can be used with many conventional microscopes, amplified staining methods, and oligonucleotide-based staining...deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National Academy of... -
What's New in CRISPR - March 2020
TypeBlog Post... Blue light renders the NLS accessible and the complex enters the nucleus where dCas9-Mxi1 can repress.... In the dark, the NLS is inaccessible and the complex remains in the cytoplasm. Read the paper in Cellular... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...could also have major applications to cancer, for example, the visualization of chromosomal translocations...Addgene. A protein-tagging system for signal amplification in gene expression and fluorescence imaging.... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post...signal-to-background ratio, thus, allowing higher resolution in samples containing substantial autofluorescence background...direction of biosensors capable of revealing, for example, how muscles work inside a living animal. For those... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog Post...2017). Type I CAST systems rely on the Cascade complex minus its nuclease component, Cas3, while Type ... systems; Cascade shown) combines with a donor template and transposon proteins (Tns) to integrate large... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog Post...kind of walking them through the steps of, for example, cutting open a heart to look at the inside. We...new technologies in education and ask ‘Can we implement that?’ [And think about] where we [eScience] are...