We narrowed to 937 results for: mpl

...metagenomic datasets to try to determine whether simpler and maybe smaller Cas systems could exist in nature... more primitive version of the larger and more complex Cas9 and Cas12 proteins. Cas14 cleaves ssDNA But...

...cassettes for selection of transgenic plants. The complete GreenGate toolkit, now available at Addgene, also...had been humming along at 99 percent efficiency, simply stopped working. They finally traced the problem...

...provide you with that control in a modular way. For example, say you would like to label neurons with mCherry...pmSyn1-EBFP-Cre Of course there are also more complicated ways to use this particular AAV. For instance...

...by adding long-read sequencing capabilities to complement our next-generation sequencing, setting new standards...Request, a service that dramatically expands and simplifies your viral vector options. Almost any AAV transfer...

...manner, but these systems have not been widely employed in vivo. The CHIME and new X-CHIME systems, developed...containing a gRNA. These transduced stem cells can be implanted in irradiated recipients to create mice with gene...

... at a local bar. It doesn't have to be crazy complicated! Listen to the full podcast for more great science... a Science Communication group started. It's as simple as getting people together at a bar. Chapter 10...

...could provide a new tool to study old and unique samples, such as those collected by Darwin on his voyage...incompatible with the need to conserve these unique samples, meaning that we know very little about these specimens...

...the service to be fulfilled and QC assays to be completed and reviewed. The timeline therefore will be as...follows:  Request review: 1–2 days Prep and QC completed: 6–8 weeks Average shipping time: 1–2 days (USA...

...can be used with many conventional microscopes, amplified staining methods, and oligonucleotide-based staining...deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National Academy of...

... Blue light renders the NLS accessible and the complex enters the nucleus where dCas9-Mxi1 can repress.... In the dark, the NLS is inaccessible and the complex remains in the cytoplasm. Read the paper in Cellular...

...could also have major applications to cancer, for example, the visualization of chromosomal translocations...Addgene. A protein-tagging system for signal amplification in gene expression and fluorescence imaging....

...signal-to-background ratio, thus, allowing higher resolution in samples containing substantial autofluorescence background...direction of biosensors capable of revealing, for example, how muscles work inside a living animal. For those...

...prepaid return shipping label. To return the box, simply peel your address sticker off the box, remove the...packaging (no coated tissue culture plates, for example). Check the link for more details on what they ...

...wrong. Whether it be a bad fridge freezing your samples, or a dysregulated water bath boiling your experiments...faced an odd situation with experiments? Our art simply mirrors our lives at this point! It was a great...

...spent collecting it will have been worthless. But simply writing down your data doesn’t guarantee a publication...portion of the paper will be generated. After you complete a publication quality figure, produce the figure...

...country that we support. This story is a vivid example of how the global scientific community’s generosity...immunogenicity and protection efficacy of self-amplifying and circular mRNA vaccines against SARS-CoV-2...

...sequence reduces the stability of the genome-bound complex. This decreases the Cas protein’s tolerance for...on-target efficacy. For perfect sequence matches, the complex is stable enough that the DNA substitution doesn...

...2017). Type I CAST systems rely on the Cascade complex minus its nuclease component, Cas3, while Type ... systems; Cascade shown) combines with a donor template and transposon proteins (Tns) to integrate large...

...kind of walking them through the steps of, for example, cutting open a heart to look at the inside. We...new technologies in education and ask ‘Can we implement that?’ [And think about] where we [eScience] are...

... editing sphere.” But things aren’t always that simple, especially when your spouse is also in science... stumbled upon eScience by luck, by chance, by complete happenstance. It was on Indeed or one of the job...