...this, scientists use a multitude of techniques including co-transfection of two or more plasmids, the use...

...encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato (pH sensors), HyPer (H2O2 ...

... molecules into regulation of gene expression including growth or fluorescence. There are a few examples...

...identification of several disease-causing genes, including the RPE65 gene responsible for an autosomal recessive...

...mammalian cells. The modules used in this method include fluorescent proteins, promoters, enhancers, polyadenylation...

...transformation method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium...

...PPIs that regulate numerous cellular functions, including ubiquitinoylation, kinase signaling, integrin ...

... ability to edit RNA has multiple advantages, including reversibility and use in non-dividing cells. With...

...confers resistance to several aminoglycosides, including G418 which is commonly used for eukaryotic antibiotic...

...insights into the cytoskeleton of malaria parasites including the conservation of a conoid. PLoS Biology 19:...

...various gram-negative bacterial hosts of interest, including within mixed communities and into species not ...

... multiple approaches. Major restaurant chains including Chipotle and Panera Bread have pledged to eliminate...

...Cpf1 found and tested? Class 2 CRISPR systems, including the type II Cas9-based system, require a single-component...

...assigning a physical location to your samples, include as much detail about that location as possible....

...pathogens including organisms such as Staphylococcus aureus or Vibrio cholerae . BSL-2 includes all of the...sharps container. Sharps include anything that can pierce your skin, including, but not limited to broken...experiment, make sure your workspace is clean and uncluttered. Disinfect your workspace with appropriate materials... all areas containing biohazardous materials, including biohazardous waste. Dispose of waste that may ...followed in addition to BSL-2 guidelines below, including PPE protocols . Working in a BSL-2 laboratory ...biohazardous waste containers that can be autoclaved. Conclusion Although simple, following appropriate BSL-1 ...

...smFPs can result in better resolution images. Conclusion By combining advantages of FPs and epitope tags...

...burden from getting overwhelming. These mechanisms include efforts like flexible staffing on case teams, where...

...activity, for example. Useful positive controls could include: pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS & pSB166...

...whether you realize it or not. These skills might include collaborating, managing, and communicating. After...

... use of other tools they use in research. This includes chat apps like Slack, Microsoft Teams, and Google...