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...between my lab in Pennsylvania and a team in Paris, France. While efforts like these accelerate the progress...everyone can access data and drafts of manuscripts and grant applications in real-time so they can provide input...International Cancer Immunotherapy Conference in Frankfurt, Germany When funding and circumstances permit...

...or A:T->G:C edits in DNA without causing double-strand breaks. Our new page shows you base editing plasmids...certainly taken notice! Our newest page in the Transcriptional Regulation category already has five blue flame...with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation: Truncated gRNAs for Regulating ...

...of neuroscience. The larvae of zebrafish are translucent and allow for noninvasive live imaging with fluorescent...that allow charged particles to cross the cell membrane when exposed to light. Neuronal firing depends... Portugues, Ruben, et al. "Optogenetics in a transparent animal: circuit function in the larval zebrafish...

...better. More transcription factor binding sites in a promoter lead to higher transcriptional activation....for single molecule imaging, finding that plasma membrane-targeted CAAX-SunTag was 18-fold brighter than...

... research project establishes your professional brand. It offers colleagues insight into your interests...understand where the field is today? Have you talked to veterans in the lab or the field? What are possible pitfalls...how can you mitigate them? If you were to write a grant for this project, what would be your critical experiments...

...sequences required for viral replication and transcription as well as reporter genes. To learn how you ... your research, check out this post! R Bodies: Membrane-Rupturing Microscopic Tools In this post, Jessica...also showed that R-bodies can rupture E. coli membranes. Read the full post for potential applications...

... synthesis companies often work within a narrow range for GC content. Rare codons also are not good predictors...sequences is Proteobacteria - the most frequent transfer of genes is from Proteobacteria to mammalian expression...natural gene sequence is, the less likely it is transferred into another organism in the lab. Predicting ...

...plasmids from the same group should not be co-transformed, so if you need two plasmids for an experiment... same incompatibility group should not be co-transformed. +Actual copy number varies. See below for ...stocks, agar stabs, or liquid cultures. Freshly transformed cells also give a higher plasmid yield that colonies...

...used to stay up past my bedtime, poring over my grandparents’ medical textbooks by torchlight under the covers... “motivated reasoning,” the catchall term for a range of neat little tricks our minds perform to avoid...difference between airborne and aerosolised droplet transmission and how different fabrics and mask designs could...

... out and onto the screen and transform your work into a paper or a grant. Here’s a list of tools and resources...in red, words with a medium frequency of use in orange, and common words with high frequency of use in...

..., K. phaffii is exceptionally good for post-translational modification of recombinant protein products...proteins. Peroxisome studies Peroxisomes are small, membrane-bound organelles essential for sequestering certain...non-homologous end joining (NHEJ) to perform DNA double strand break repair instead of homologous recombination...

... The space was modern with clean bold colors of orange and white. The decor was very contemporary and ...components. Without proper processing, these small DNA strands can form concatemers in later steps. This new NEB...hours. It was robust, capable of generating a wide range of insert sizes and also produced high yields of...

...particular study, which includes comparing the transduction patterns of different AAV serotypes. To help... Biosensor AAV The ability to detect the neurotransmitters and other bioactive molecules is integral ...ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120  AAV1 pAAV-ihSyn1...

...ready-to-use lentivirus from Addgene, you’ll also receive transfer plasmid DNA that you can use along with lentiviral...Addgene Blog Get the Most from Your Lentiviral Transdution Using Virus in Your Research: A Primer for Beginners...Beginners 5 Tips for Troubleshooting Viral Transductions Additional Resources on Addgene.org Visit the...

...Resistance gene and Selection Markers) and one “Transfer Plasmid” game board. Once they agreed on each ...had 35 minutes to develop a strategy and build a transfer plasmid that could be used to genetically manipulate... this way, the students also had to practice transferable skills like team building, collaborative critical...

... 3’ end. This repeat region associates with the trans-activating crRNA (tracrRNA) which interacts with... important cellular RNA molecules with post translational modifications. One of the most common of these...targeting experiments allows you to visualize transfection efficiency and even track localization in cellular...

...Fragmenting  The next step is break the DNA into random, small pieces of roughly equal size, typically ...enzymatic digestion. Sonication provides a truly random digestion, but is harsher and requires more optimization...your protein of interest is a low abundance transcription factor or cofactor. In such cases, the gentler...

...that helps attack extracellular pathogens.  Transportation of antibodies to places they can’t reach on...region, can be bound by special receptors that transport antibodies through cells and into different body...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ...

...expression, researchers often need to determine these transduction patterns empirically for their own experiments...AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP Brandon Harvey 50457 AAV2 pAAV-hSyn-DIO-EGFP Bryan Roth...system takes advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression...

..., spike proteins, envelope small proteins, and membrane proteins. Although all viral components are available...glycoproteins and other proteins that require post-translational modification may not be easily expressed in ...sequencing as a first pass QC. The lab team manually transforms, innoculates, and performs DNA minipreps for ...