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...blots are a great tool to identify a protein of interest in a complicated solution like cell lysate. But...

...You’ve done great research and made interesting discoveries. You’ve analyzed the data and generated beautiful...

...directional gene transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer...

...as soon as you can. It is in your supervisor’s interest that you succeed, and if you cannot resolve a ...

...your own LB/agar plates with your antibiotic of interest. Last Upload: September 14, 2016 Protocol Video...selecting only bacteria containing the plasmids of interest (for example, fewer satellite colonies will grow...

...plasmids — a transfer plasmid containing your gene of interest plus two packaging plasmids (three packaging plasmids...

...just a starting point for much more. If you’re interested in working on these questions or collaborating...

...Colloids and Interfaces. Sana is particularly interested in Glycobiology and Immunology. You can find ...

... of your poster visitors had a great idea or interesting questions worth discussing with your lab. If ...

...new cells are better at producing a compound of interest, thus allowing them to optimize the compound production...

...undergo as it interrogates your genome. If you are interested in experiencing Cas9 up close and personal, visit...

...Regenerative Medicine (CRM), Edinburgh. His research interests include optimizing genome-editing methods in brain...

...staggering. The process is complicated. If you’re interested in the drug discovery process, or you want to...

...with. In either case, it can be useful to run a restriction digest of your amplified library on a gel to ...

... information about this gene or DNA element of interest. If the depositor has indicated that there is ...

... protocols, including making LB agar plates, restriction digest of plasmid DNA, and bacterial transformation...

...- a common method for visualizing molecules of interest within a cell or tissue. In that introduction,...

...was free of agarose and could be digested with restriction enzymes.  Cellulose nucleic acid purification...

... to sometimes result in cell death, has led to interest in using adenoviral vectors for vaccine delivery...

...distribution as DNA (Trimmer, 2020), you may be interested in using them in your own experiments. It is ...