We narrowed to 838 results for: tat
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TypeBlog Post...resembles cas10. All type IV subtypes are missing adaptation genes, Cas1 and Cas2, which are critical for ... become the most engineered Cas enzyme, with adaptations for increased fidelity, activation/repression...
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Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...cells used and the readout, this could be a major limitation. An alternative to the full genome CRISPR screen...PhD student. In particular, one slide of the presentation made a lasting impression on me. The slide showed... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post...being able to specify and correct single-base mutations without introducing DSBs at all. Base editing ...are smaller and less likely to cause off-target mutations than those derived from naturally occurring cytidine... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...lane 1 is lane 8. Common ways to keep membrane orientation straight are to mark the upper left-hand corner... Ensuring wash is happening under sufficient agitation (rocking) Add Tween-20 to wash buffer (can... -
New Antibodies in the Lab Animation!
TypeBlog Post...study proteins via ELISAs, western blots, immunoprecipitation, immunohistochemistry, immunocytochemistry... -
Plasmids 101 eBook 4th Edition
TypeBlog Post...they go to Addgene.”-Ryan Mehl, Professor, Oregon State University. ... -
Addgene’s First AAV Data Hub Challenge
TypeBlog Post...be invited to compete in our speed round data presentation, with two winner each receiving a $500 travel... -
2022 Depositor Week
TypeBlog Post... Monday: Thank you post (this is it!) Tuesday: Stats roundup on Twitter Wednesday: 2022 Blue Flame Depositors... -
Introducing the Addgene Antibodies 101 eBook!
TypeBlog Post...eBook collection useful. And, as always, don’t hesitate to reach out to [email protected] with any thoughts... -
Expanded Access to Rabies Plasmids
TypeBlog Post...Addgene shipping and ordering processes, don’t hesitate to contact our friendly customer support team ... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog Post...achieving it. Once they designed the protein computationally, the researchers expressed, purified, and characterized... -
Addgene Protocols Go Viral: AAV Purification and Titration
TypeBlog Post... published an article describing a sucrose supplementation strategy to improve yield. In addition, as ... -
New Podcast Segment: Hot Plasmids
TypeBlog Post... new tool for making diverse, localized point mutations. Listen to Our Segment on CRISPR X Hot Plasmid... -
CRISPR Cheat Sheet
TypeBlog Post...cells are screened for phenotypes of interest. Limitations of CRISPR Precise edits are difficult. CRISPR... -
Depositor Week 2020 is Happening Now. Join us!
TypeBlog Post... Here's the schedule for the week: Monday: Fun stats and trivia begins! Tuesday: Depositor features (... -
AAV Packaged on Request is Here!
TypeBlog Post... convenience. After an order is placed, we’ll facilitate the necessary MTA, then start production and ... -
Newly Updated AAV Data Hub!
TypeBlog Post...standards (* above) will be given a DOI for easy citation by others and, when published, will meet FAIR ... -
2018 #DeckTheLab Contest Winners
TypeBlog Post... year, @BordeauxFoSho takes you through our interpretation of Buddy the Elf's journey from the North Pole... -
Editor's Choice, July 2016
TypeBlog Post...for Gene Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo As... -
Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs
TypeBlog Post...mechanisms behind interactions between crops like potatoes and their microbial pathogens. This work has given...