...After a virion infects a cell, the single particle creates many copies of itself, sometimes up to millions...infected cell.) This is in contrast to bacteria and eukaryotes, which use a binary replication process whereby...

...fields of biology and enable the conversion of RNA templates to DNA but suffer from several important shortcomings...faithfully transcribes RNA to DNA with low error rates compared to other RTs. MarathonRT represents a major...

...again until you don’t.” As every researcher can attest to, science is nothing if not solving your way ...creativity, changed the outcome of the project, or illustrates the value of the work and then build a story ...

...KSQ uses a CRISPR-Cas9 platform to systematically test the function of all 20,000 genes in disease models... of Oncology Target Discovery at the Novartis Institutes for Biomedical Research. Drug development was...

...faster with technology transfer offices (TTOs). The latest of these efforts is the Golden Stopwatch Award ...Montana State University  National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)  National...

...KAUST, Saudi Arabia, in the group of Prof. Mark Tester, where she focuses on (1) salt-induced changes ...Credibility, peer review, and Nature, 1945–1990." Notes and Records: The Royal Society Journal of the History...

...-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent protein ...through successive rounds of mutagenesis for the brightest teal fluorescent proteins that were photostable...

...applied for identification of essential MYC binding sites in a wide range of human cells.  A new pooled library...targeting start codons, splice acceptor and donor sites, and introducing premature stop codons.    New...

...to the lysosome, and degradation of autophagic substrates inside the lysosome. Historically, methods for...the cytosol, this ratio is low; but when Keima accumulates in autolysosomes, this ratio is high. When untargeted...

... was natural or synthetic 97.7% of the time in a test set of 173 known synthetic and natural genes.  ...saw that a percent sequence identity below 85% correlates with synthetic sequences. The researchers then...

... described by Li ZY, et al. PLoS One, 2016) and tested a series of mutations. Through this process mCarmine... mNeptune684, and has a favorable pKa of 5.6. To test out this new FP in your own experiments, mCarmine...

...detection algorithm that tolerates occasional mismatches or indels. Empirical tests indicated that a reasonable...

... Dangerous Viruses Contributed by guest blogger Tessa Cressy from the labs of Dr. Elke Mühlberger and ...Medical School describes how AAVs function and indicates important things to consider if you’re getting...

...diseases, offering them the chance to win some of the latest tools and technologies in life sciences research... other genes involved in photosensitivity in melanocytes with the goal of understanding both XP and melanoma...

...been limited to the overexpression of various metabolites in Escherichia coli or Saccharomyces cerevisiae...regulator or the number of designed scaffold binding sites. With hundreds of characterized aptamer domains ...

... for marketing positions posted on recruitment websites, this job specifically looked for a researcher...think of to start was “Marketing for Dummies”. The fastest way to learn something about marketing would be...

...neurons at once. Addgene spoke to Boyden about this latest optogenetics advance, the big questions that motivate...neuron or set of neurons, you can see how it contributes to behavior or pathology, and by disabling neurons...

...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...instead of room temperature or 37 °C. The protocol notes when there are different incubation options available...empirically determined. If possible, run an initial test with serially diluted primary antibody and determine...Equipment Spectrophotometer compatible with 96-well plates 1–10 µL single channel pipette 2–20 µL single channel...VWR 76322-150 Pipette tips, 20 µL, VWR 76322-134 Pipettes, 10 mL, VWR 89130-898 1 L polystyrene bottle, ...display a green product. A precipitated product indicates the presence of too much HRP and the need to optimize... and incubated overnight with Anti-Desmin [D7]. Plates were incubated with HRP-linked anti-Human IgG secondary...

...sequence containing transcription factor binding sites can increase transgene expression and transduction...validate ITR sequences as these enzymes cut at known sites within the ITR. However, small deletions and point...

...under a new promoter each time a new hypothesis is tested. Controlling multiple genes with Gal4/UAS system...Targeted gene expression as a means of altering cell fates and generating dominant phenotypes., Development...