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... color to achieve the highest color variations. Adjust the plasmid concentrations or AAV titer for the...

...end cloning. So how does TOPO cloning work? As illustrated in the Figure below, the “A” overhang on the ...

...into the cells of interest. In contrast, RNA guides must be expressed from a plasmid or in vitro transcribed...

...popularity of our career posts shows that we are doing just that. Do you like the direction the blog has taken...

...and topology, and cloning and gene expression. Just released: Addgene's Plasmids 101 (2nd Ed.) eBook...

...cell behavior in greater detail. The goal is not just to understand the disease better but hopefully also...

...added, deleted, or reordered and PCR enzymes are adjusted. Appropriate fragment and primer design results...

...hACE2 transgenic mouse strain, for example, is sustained by the availability of this strain from The Jackson...

...you only need 1-2 ul of sample and the run time is just a few minutes per sample. During the run, DNA fragments...

...dynamic range. However, the promoter in question must be σ54-dependent and the system may not be adaptable...

...contributed by Eric Perkins Mammalian genomic DNA is not just a 2-D linear molecule snaking through our cells’...

...ineffective or require several rounds of dose and drug adjustments. Unger ultimately hopes that medical professionals...

...Over the past few years, dCas9 has also become a robust visualization tool to study the spatial and temporal...

.... In applications when repetitive tRNA sequences must be avoided, different tRNAs with optimized 5’ leader...

...fluorescent proteins, for example, as many viral particles must infect a cell to achieve visible expression of the...

...biological reactions that I learned about from textbooks just made everything click in my head and left me thinking...

...they had 10 minutes to expose their strategy, justifying each single element used to build the construct...

... Have you ever found yourself frustrated reading through a paper to find that there is insufficient information...

...or PAM. In order for dsDNA to be cut by Cas9, it must contain a PAM sequence immediately downstream (3...

...light-controlled gene expression in E. coli easier and more robust. Synthetic photobiology: why use light? In synthetic...