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...Comparison of TnpB (A) and Cas9 (B) genome editors. Effector nucleases are shown bound to the target DNA...AMPA.FingR (AF) that binds to AMPA receptor subunit GluA1 found at excitatory synapses (Rivera & Huang, et ...plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles. Here's what...components (nucleus, endosomes, lysosome, golgi, ER, inhibitory synapses, and cytoskeleton components) and in... household name, the search for smaller genome editors is heating up and TnpB has found itself in the ...but TnpB is small enough to be carried by viral vectors (~400 amino acids, less than a third of the size... (2025). Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis...

... you want the works: organizing reagent storage and inventory, managing orders, tracking students and ....io beta platform for sharing, annotating, and storing life science protocols. Since we began sharing ...website in December 2006, the pLKO.1-TRC cloning vector (deposited by David Root of the Broad Institute...Institute) has consistently been one of the repository’s most frequently requested plasmids. I wondered how many... and you decide to share them using Addgene’s repository, you can also share your associated protocols...listed under “Supplemental Document”: gRNA_Cloning Vector (deposited by George Church’s lab). Addgene has...the software aims to be a life science protocol repository. The software can be used with the ZappyLabs ...

... insert and vector are first PCR amplified. The forward primer used to amplify the vector is located in...your vector and insert. Once you’ve confirmed that the PCR was successful, mix together the vector and ... PCR with vector specific primers to confirm that the products are the correct size (vector + insert)....specific sequences for base pair overhangs, insert and vector purification, and, most importantly, excessive ..., it does not require purification of insert or vector products, nor does it require the use of specific...process in brief In FastCloning, you PCR both the vector and the insert in separate reactions and then combine...bases that are complementary to the overlapping vector ends. See an overview of the PCR process and the...

...Professional to mentor and coach you. There are many grant consultants who offer grant training, mentoring and coaching...to late-career grant professionals have similar stories about a time when their passion for an organization...their new career path.  A part of the career path story for most established grant professionals is learning...commitments to and proof of compliance with the grantor’s requirements as well as state and federal regulations...amount of grant funds available and the increasing regulatory process for grants has fostered the demand for...professional. Dr. Cook served as a principal investigator for several research grants. One of the benefits...benefits of being a principal investigator is that you are asked to review grant proposals from others. ...

...guide to viral vectors Find all of our viral vector blog posts Download our viral vectors 101 eBook Additional...that this approach can be adapted by vector cores and laboratories alike as an easy and inexpensive method...pressure on the production of retroviral vectors: Enhancement in vector stability." Biotechnology and bioengineering...been actively investigating ways to improve viral vector production. Whether redesigning viral packaging...membrane When packaging adeno-associated viral vectors (AAV) not all serotypes are equal; while some serotypes...cells by treating the cells with the endocytosis inhibitor sucrose. Previous work by Stoneham et al. demonstrated...less time and resources resupplying our current inventory and more time providing new materials to the research...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...Catalysis by TEV protease allows a transcription factor to enter the nucleus and modulate transcription... a cell. The Goedhart lab, Gadella lab, and collaborators have an ongoing effort to improve genetically...pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector commonly used for in vivo biotinylation via BirA...Institute developed fluorescently labeled piggyBac vectors for uniform and sustained expression of single ...multiplexed gRNAs and of dCas9 fusions. The dCas-KRAB activator and the dCas-VPR repressor are introduced into...an EGFP reporter. Expression of the dCas9-KRAB activator or dCas9-VPR repressor in hPSCs is also driven...

...into small, well defined chunks, called stories, and work on stories during short periods of time called ...clarifications lead to the story being scrapped or broken up into smaller stories. Since this occurs before...plasmids from the moment a deposit kit is sent to a depositor, to streamlining the order fulfillment process... sprints. The team is mindful of the number of stories we commit to for each sprint, to ensure we will...process for working on a new feature. We start with a story that describes the feature and what the criteria...accepting the feature as done correctly. These initial stories are usually a result of collaborations between ...stakeholders (other Addgenies). Once we begin work on a story, we continue to collaborate closely with each other...

...joined Elsevier after her postdoctoral studies, and has held various editorial roles on review and primary... of the Cell Press editorial department, a team of around 100 professional editors who produce a broad...contributed by Deborah Sweet, Vice President of Editorial at Cell Press. Almost everyone who works in a ...filtration system, environmental stability in an incubator), but there’s a lot you can do to help other scientists...shoulders of journals and publishers. As journal editors, we often find ourselves having to ask authors ...paper off their desk and move on. Nevertheless, editors can and do play this role, and we take our responsibility...Press. Deborah Sweet is the Vice President of Editorial at Cell Press.  She trained originally in cell...

...expression vectors which are often more difficult to transfect or package into viral vectors. Cas9 multiplexing...multiplexing vectors are larger because they require regulatory sequences to allow for expression of multiple... 1) Most rely on transfection of more than one vector to express the gRNAs and Cas9. Co-transfections...Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and up to 7 gRNAs Each gRNA requires...Expression Cassettes lentiviral transduction One vector expresses Cas9, eGFP, and up to 4 gRNAs Each gRNA...plasmids resulting in smaller, more streamlined vectors which are easier to use across multiple expression...In most of Zetsche et al’s experiments, a single vector co-expressing Cpf1 and a crRNA array were used....

...stimuli are processed by a network of excitatory and inhibitory neurons whose function and connectivity...She fused fluorescent protein tags to the receptors to monitor their localization and expression. Qiu saw... are working on developing new surface display vectors to improve expression and trafficking of these ... that is then transferred to thiol-containing acceptors. These hydropersulfides modulate protein activity...have important roles during oxidative stress. To monitor this activity, Pedre Pérez’s biosensor fuses MPST...can also be used along other thiol-containing acceptors in competition assays. “I believe that improved...where she engineers genetically encoded voltage indicators. Tian developed a video-based high-throughput...