We narrowed to 997 results for: lars;

...codons altogether.  So what does this mean for a molecular biologist who wants to express genes from one ...might want to optimize codons for expression in a particular organism. Why is codon usage important? Translation...tRNAs to build a peptide. Image from Mariana Ruiz Villarreal. By the late 1980s, scientists even created... vivo (Slimko and Lester, 2003).  Addgene has a large selection of codon optimized genes, so check out...information encoded in the primary sequence of a particular gene which could affect translation initiation...optimization in human therapeutics. Trends in Molecular Medicine 20:604–613  https://doi.org/10.1016/j.molmed...Resources on Addgene.org Check out Addgene's Molecular Biology Reference Read about genetic code expansion...

...A similar genetic code is used by most organisms on Earth, but different organisms have different preferences...reasons include: Metabolic pressures - it takes cellular resources to produce tRNAs that recognize different...contains a gene that’s been codon optimized for a particular organism, this will sometimes (but not always... purification procedures. Even if you produce a large amount of protein in your expression host of choice...cotranslational folding." Nature structural & molecular biology20.2 (2013): 237. PubMed PMID: 23262490...bias as a means to fine-tune gene expression." Molecular cell 59.2 (2015): 149-161. PubMed PMID: 26186290...featured topic page Resources on Addgene.org Molecular biology reference pages Genetic code E. coli strains...

...publications Online tools like ResearchGate, GoogleScholar, Academia, ORCID, and MyScienceWork all help... especially published research, have grown in popularity over the last decade. Research by Scott Spicer...abstracts identified. The journal Cell had the largest average view count per video abstract at 2,947 ...being used to share science. TedTalks are very popular, though they focus mostly on highly innovative ...even use a computer.  Currently, two of the more popular biology videos are:     “Singing” Fish Rely ...Ryan at [email protected]   References 1. Larivière, Vincent, Yves Gingras, and Éric Archambault. ...and case study." Journal of Librarianship and Scholarly Communication 2.2 (2014): 2. Additional Resources...

...scientific or scholarly research contributions in the field Evidence of authorship of scholarly books or articles...should not be relied upon for legal advice in any particular circumstance or fact situation.  The information...agencies to propose reforms to the H-1B visa system. Large firms and firms that work on projects with an international... extraordinary ability category The EB-1A - is similar to national interest waiver criteria however the...showcases (not usually applicable to scientists). High Salary –not relevant to most postdocs. Commercial successes... articles (in scholarly journals with international circulation) in the field EB-1Bs historically have...documents that are not in English. Create a Google Scholar account Sanitize your LinkedIn, Facebook profile...

...Biology have shown similarly disappointing rates of reproducibility. These alarming results are due, at...publications and funding. If any of these three pillars crashes, the lab's success and the careers of its...effective, and our schedules more organized. From the alarm in the morning, to the daily news briefing, to quick...solution that fits the most labs, our team conducted a large study which focused on the adoption of ELNs in labs...talking about ELN costs and limited budgets, “a large percentage of survey respondents indicated that ...support that. In addition, if you are working for a large institution or company, site licenses might be available...

...to efficiently deliver large repair templates for applications that require large gene fusions. The current...vehicles for episomal gene expression and are particularly useful for gene delivery to the nervous system...is composed of single stranded DNA, which is particularly well suited for strand invasion, a key step ...edit your gene of choice. These vectors are very similar, both with polylinkers for the addition of gene-specific...gene targeting in human pluripotent stem cells." Molecular Therapy20.2 (2012): 329-338. PubMed PMID: 22108859...Matthew H. Porteus. "CRISPR-mediated integration of large gene cassettes using AAV donor vectors." Cell reports...

...plasmids together on a tight budget. To help popularize our kit resources,  we’re highlighting just a...seven-repeat proteins: Sjuts, Hanno, et al. "Molecular basis for inhibition of AcrB multidrug efflux ...open (2016): bio-017129. Hsia, Justin, et al. "Modular Synthetic Inverters from Zinc Finger Proteins and...translation initiation sequences are optimised for a particular system. The Alexandrov lab at the University ...University of Queensland’s Institute for Molecular Bioscience has provided a solution for this problem by developing...changes in enzyme function can lead to surprisingly large fitness effects during adaptive evolution of antibiotic...specific RNA bases. By swapping out these different modular repeat segments, you can create a PUF protein that...

...relatively low postdoc salaries as opposed to Staff Scientists Increase the Starting Salary for Postdocs to ...organizers wanted to touch on in organizer David Riglar’s blog post. Essentially the goal was to determine... base of all these questions as there is not particularly good data on how many post-docs there are in...positions outside of academia Require that postdoc salaries be paid on fellowships instead of grants such ...to $50,000 (the current median postdoc starting salary as reported by Paula Stephan is $40,000) Increase... Found Here Resources at Addgene  Read David Riglar's FOR Boston 2015 Blog Post Read Our Career Blog...

...personnel salary ranges, go to online job information sites (like, indeed.com, or salary.com). Search ...institute is located. For example, salaries in Kansas versus salaries in NYC will vary widely. Therefore...institution/funding source. Major equipment includes large centrifuges, sequencers and analyzers, and any ultra...candidates, and, more importantly, it’ll help you define salary costs. 4. Ascribe a cost structure associated to...Therefore, start up awards should reflect salaries in the region you’ll be working. At this point, your budget...

...Double-Strand Break Repair in Mammalian Cells. Molecular and Cellular Biology, 18(1), 93–101. https://doi.org...search for "CRISPR", short-hand for Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed...Matt Ferenc, to organize the CRISPR forum's most popular questions and answers. Designing your CRISPR genome...introduce cleavage of the target site with efficiency similar to using a single chimeric gRNA. At the same time...with the Cas9/sgRNAs vector (like PX330). For large changes (>100 bp insertions or deletions), we typically...lab typically use PAGE purified long oligos. For large changes (>100 bp insertions or deletions), the Zhang...Addgene blog CRISPR Cheat Sheet Build Your CRISPR Vocabulary CRISPR 101: Off-Target Effects Visit the CRISPR...