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  1. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ... using biorender.com   If you’re new to talking about viruses, here’s a quick tip on terminology. “Virus...have a growing number of Viral Vectors 101 posts about each type, so be sure to check our blog, or our ...

  2. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...Tags have a lot of major benefits – as discussed above – but there are a few instances when they should... hindrances, and destabilization can be brought about by the tag so it’s important to validate tagged ...

  3. RUBY-Red Siliques

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    Blog Post
    ... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...resistant disease. (RUBY-red: You can’t beet it!) About the Author  Andrew Willoughby is a graduate student...

  4. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...naturally.  Sample Denaturing  First, let’s think about induced conformation changes. Several types of assays...immunoimaging assays. It can also elucidate information about what a given protein is doing or changing in different...

  5. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 114472  AAV5...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  7. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...last updated Nov 10, 2020. Now that we know all about antibiotic resistance genes, let’s consider another...the replicon. For example, the pMB1 ori maintains about 20 copies per cell, while pUC – which differs by...

  8. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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    Blog Post
    ...Our work accelerates and enhances productive collaborations as well as provides visibility for grant-driven...research is accelerating essential research in laboratories all around the world." We thank the Chan Zuckerberg...

  9. Plasmid Cloning by PCR

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    Blog Post
    ...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time...Additional Resources on the Addgene Blog Learn About Other Cloning Techniques Catch Up on All Things ...

  10. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...getting together and chatting with another person about their career path is a great start to a mentoring...Medical School researcher Connie Cepko to learn about her mentoring style. Set goals with a mentor: The...

  12. Science Career Options

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    Blog Post
    ...skills and experience.You might not normally think about it, but hobbies and volunteer experiences, as well...show that you have some of the abilities listed above. Showing that you are willing to exercise these ...

  13. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...conduct the screen. To help alleviate some of this labor for our users, we now provide the Brunello (targeting... hear from you if you have used or are thinking about using our viral services. Please send any questions...

  15. The MTA at Addgene

    Type
    Blog Post
    ... who aren’t familiar with it. If you’re curious about the hows and whys of the material transfer agreement...easier for others.  If you’ve still got questions about our MTA process, you can check out our Tech Transfer...

  16. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ... it can be used in a lab? Here we’ll be talking about the ins and outs of this classification system, ...The classification system is also useful in a laboratory setting. When designing a plasmid to be inserted...

  17. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...brightness after ~100 action potentials. This is about twice as bright as GCaMP7f and GCaMP7s. See all...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  19. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 37825  AAVrg...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...
Showing: 681 - 700 of 963 results