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  1. CRISPR in the Clinic

    Type
    Blog Post
    ...mutant allele. When all was said and done, the researchers had cured 24 mice of their disease. Meanwhile...traditional gene therapy pipelines (viral), and researchers are currently working on giving this treatment...

  2. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...targeting. By employing various AAV serotypes, researchers can precisely direct the calcium sensor to specific...complex brain circuit dynamics in vivo. Neuroscience Research. 2021 Aug 1;169:2-8. Suzuki J, Kanemaru K, Iino...

  3. A Look at Addgene's QC Process

    Type
    Blog Post
    ...the accumulation of errors in DNA sequences. In research, these errors can affect experimental processes...importance of QC Plasmids are a fundamental part of research. They are easy to produce, easy to edit, and easy...

  4. Design Tips for Prime Editing

    Type
    Blog Post
    ...! First, a quick refresher: prime editing is a “search and replace” genome editing technique that relies...Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand...

  5. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...switches were designed as a genetic tool for researchers to conditionally manipulate gene expression in...allowing for expression of GeneR. This allows a researcher to test whether or not expressing GeneR alone...

  7. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...FRET based tools to study epigenetics Epigenetic research uses a wide range of molecular biologic techniques...none of these techniques can be used for in-situ research of dynamic modifications in real-time, posing ...

  8. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ... the battle when using optogentics to answer a research question. The flip side of the optogenetics coin... molecules in freely moving mice. Neuroscience research. 2011;70(1):124-7. doi: 10.1016/j.neures.2011.01.007...

  9. Hot Plasmids February 2024

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    Blog Post
    ...fluorescent proteins derived from StayGold. ResearchSquare (Preprint). https://doi.org/10.21203/rs.3.rs... brilliance By Hridaya Patel (Addgene co-op) Researchers in the Gradinaru lab have developed an engineered...

  11. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ... A recent survey of PhDs found that many researchers feel that they lack formal training in a variety...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working...

  13. Truncated gRNAs for Regulating Gene Expression

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    Blog Post
    ...bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss Institute for Biologically Inspired...University of California, Santa Cruz, where her research is focused on engineering nanoparticles as drug...

  14. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...resistance gene can affect a cell's phenotype, researchers usually remove it after selection using the Cre...conditional knockout. To make a conditional knockout, researchers often use the Cre/Lox system described earlier...

  15. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... of a gene exists, there must be assays that researchers can use to quantify said copies. They do so using...commonly available and may not be an option to all researchers. Accuracy needed Do you need to call exact...

  16. Transferable Skills: Negotiation

    Type
    Blog Post
    ...A 2017 survey found that many researchers feel they lack formal training in a variety of transferable...set out to fill this gap by highlighting that researchers learn many transferable skills while working ...

  17. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid library encompassing...encapsulating a single enzyme. Compared with previous research, which required protein compartments to co-localize...

  18. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...of CRISPR-based immune systems in bacteria and archaea. Class 1 is further broken down into different ...ubiquitous in nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...

  19. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...recent studies (Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted...Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand...

  20. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...has a research background in CD8+ T cells and cancer immunotherapies. She is currently a research technician...
Showing: 681 - 700 of 923 results