We narrowed to 802 results for: Clu

1. Viral Vectors 101: Producing Your rAAV

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   Blog Post

   ...can obtain excellent quality, high titer rAAV (including Addgene!), but you can also generate rAAVs in ...

2. Polymerase Chain Reaction Overview and Applications

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   Blog Post

   ...qPCR. qPCR is used in a variety of applications including gene expression profiling, studying copy number...

3. Hot Plasmids and Viral Preps - May 2021

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   Blog Post

   ...our recombinase-dependent virus selection to now include Flp-dependent DREADDs viral vectors. Both inhibitory...

4. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   Blog Post

   ...chromatin interaction networks since they are a large cluster of enhancers. CAPTURE 2.0’s multiplexing and high...

5. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   Blog Post

   ... xCas9 2.0-2.6. This second round of evolution included continuous outflow of phage, increasing stringency...

6. Academic vs. Industry Postdocs

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   Blog Post

   ...Many companies have industry postdoc positions, including AstraZeneca, Pfizer, Novartis, Biogen, Amgen, ...

7. Cloning Mammalian Cells with the Agarose Method

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   Blog Post

   ... will die. If the solution is too cold, it will clump prematurely, and not spread out onto the plate. ...

8. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   Blog Post

   ...all of the differentiated cells in an organism, including the embryonic and extraembryonic cell types. In...

9. Tips for a 1st time AAV user (by a Rookie AAV user)

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   Blog Post

   ...… A no template control (NTC) should always be included. While the physical titer generated by qPCR is...

10. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post

    ...mind that there are many other cloning methods - including some that rely solely on PCR, and a host of commercially...

11. The Scientific Conference Poster Session: Tips for Success

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    Blog Post

    ...Cancer Research Annual Meeting, you might consider including more background on how your virus of interest ...

12. Experimenting with New Careers while in Grad School

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    Blog Post

    ...multiple avenues for professional development including workshops and networking events. I took advantage...

13. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ...additional resources provided by the depositor including genbank files and protocols here. Articles Citing...

14. Genome-wide Screening Using CRISPR

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    Blog Post

    ...available in a 1-plasmid system, in which Cas9 is included on the gRNA-containing plasmid, or a 2-plasmid...

15. Which Fluorescent Protein Should I Use?

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    Blog Post

    ...encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato (pH sensors), HyPer (H2O2 ...

16. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post

    ... molecules into regulation of gene expression including growth or fluorescence. There are a few examples...

17. Getting to Know Expansion Microscopy

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    Blog Post

    ...insights into the cytoskeleton of malaria parasites including the conservation of a conoid. PLoS Biology 19:...

18. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    Blog Post

    ...PPIs that regulate numerous cellular functions, including ubiquitinoylation, kinase signaling, integrin ...

19. CRISPR 101: RNA Editing with Cas13

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    Blog Post

    ... ability to edit RNA has multiple advantages, including reversibility and use in non-dividing cells. With...

20. MXS Chaining

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    Blog Post

    ...mammalian cells. The modules used in this method include fluorescent proteins, promoters, enhancers, polyadenylation...