We narrowed to 802 results for: Eng

1. How Addgene Creates Content

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   Blog Post

   ...and your research community! We work to provide engaging and informative resources that answer questions...

2. Hot Plasmids - May 2022

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   Blog Post

   ...Leeson Multiplexed images in live cells remains a challenge due to multiple factors, including limitations...

3. Antibodies 101: Isotypes

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   Blog Post

   ...number of constant domains, and the hinge region length. Now for some essential isotype nomenclature. Antibody...

4. 7 Tips to Secure a STEAM Internship This Summer

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   Blog Post

   ... while you make the ultimate case: why you? 7. Strength in numbers Encourage your close peers to come ...

5. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   Blog Post

   ...the Toettcher Lab, OptoNBs are photoswitchable engineered nanobodies whose binding of their target protein...

6. Screening for Successful Genome Editing with Digital PCR

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   Blog Post

   ...cleavage assays to detect mutations triggered by engineered nucleases. G3 (Bethesda) 5, 407–415 (2015). PubMed...

7. 15 Years of Addgene: The Top 15 Plasmids

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   Blog Post

   ...Cas9 (SpCas9) has been widely popular in genome engineering. For use in human cell lines, the Zhang lab codon-optimized...

8. Improved Plasmid Maps Powered by SnapGene

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   Blog Post

   ...primer names, sequences, binding site locations, lengths, and direction. Clicking on a particular primer...

9. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   Blog Post

   ...including the specs of your microscope (excitation wavelength, available emission filters, and detector sensitivity...

10. Plasmids 101: Methylation and Restriction Enzymes

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    Blog Post

    ...specialized E. coli strains have been specifically engineered to be Dam and Dcm methylase-deficient, and, as...

11. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    Blog Post

    ... Besides being fast and efficient, what other challenges does this system help to solve? Myers: Only a...

12. Plasmids 101: Blue-white Screening

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    Blog Post

    ...practical use in the following way. Scientists engineered a multiple cloning site (MCS) into the α-peptide...

13. Dos & Don'ts When Publishing a Scientific Manuscript

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    Blog Post

    ...the journal. Ask colleagues (preferably native English speakers) to proofread your manuscript and help...

14. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    Blog Post

    ...current position Understand your interpersonal challenges if they know some of the people you work with...

15. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    Blog Post

    ...genomes of mammalian or other cells. TALENs are engineered proteins that comprise a DNA binding domain and...

16. Viral Vectors 101: An Introduction to AAV

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    Blog Post

    ...and a self-complementary AAV (scAAV) vector. Full-length ssAAV vector genome of both polarities are rescued...

17. Transferable Skills Guide: Public Speaking

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    Blog Post

    ...gives your entire audience a chance to comfortably engage with you. If you can give a seminar, lead a journal...

18. The time and cost required to make a plasmid

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    Blog Post

    ...these rough estimates, it’s clear that community engagement with and use of repositories like Addgene provides...

19. Plasmids 101: The protein expression toolbox

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    Blog Post

    ...promoters Tetracycline-dependent promoters are engineered by placing tet response elements upstream of ...

20. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post

    ...Galvan et al., 2019). The hM4Di receptor is an engineered Gi protein-coupled receptor, one of the Designer...