Components of CRISPR/Cas9
Type
Blog Post
... endonuclease function, when used for genome engineering, the only CRISPR protein required is the Cas9...
We narrowed to 809 results for: Eng
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Management for Scientists: Delegating is Key
TypeBlog Post... in the organization. This will result in more engaged, successful employees who can accomplish great ... -
Resume Writing for Non-academic Science Careers
TypeBlog Post... one, one of the biggest changes (and perhaps challenges) is the need to present yourself using a resume... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...the Toettcher Lab, OptoNBs are photoswitchable engineered nanobodies whose binding of their target protein... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...Cas9 (SpCas9) has been widely popular in genome engineering. For use in human cell lines, the Zhang lab codon-optimized... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...primer names, sequences, binding site locations, lengths, and direction. Clicking on a particular primer... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...including the specs of your microscope (excitation wavelength, available emission filters, and detector sensitivity... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post...cleavage assays to detect mutations triggered by engineered nucleases. G3 (Bethesda) 5, 407–415 (2015). PubMed... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog Post... while you make the ultimate case: why you? 7. Strength in numbers Encourage your close peers to come ... -
How Addgene Creates Content
TypeBlog Post...and your research community! We work to provide engaging and informative resources that answer questions... -
Hot Plasmids - May 2022
TypeBlog Post...Leeson Multiplexed images in live cells remains a challenge due to multiple factors, including limitations... -
Antibodies 101: Isotypes
TypeBlog Post...number of constant domains, and the hinge region length. Now for some essential isotype nomenclature. Antibody... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post... Huang, N., Preston, G., Drouin, L. M., Jia, N., Deng, J., Hebben, M., & Liao, J. (2024). A Comprehensive... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...GCE4All is a community resource which depends on engagement from the scientific community to fulfill their... -
Viral Vectors 101: Types of viruses
TypeBlog Post...high immunogenicity, but some strains have been engineered to induce a lower inflammatory response within... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...specialized E. coli strains have been specifically engineered to be Dam and Dcm methylase-deficient, and, as... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post... Besides being fast and efficient, what other challenges does this system help to solve? Myers: Only a... -
Plasmids 101: Blue-white Screening
TypeBlog Post...practical use in the following way. Scientists engineered a multiple cloning site (MCS) into the α-peptide... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post...the journal. Ask colleagues (preferably native English speakers) to proofread your manuscript and help... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post...current position Understand your interpersonal challenges if they know some of the people you work with...