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We narrowed to 1,141 results for: Gene

Showing: 681 - 700 of 1141 results

  2. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...Oregon created mTFP1 by transforming E. coli with a gene library of approximately 484 variants, including... your research Subscribe to the Addgene blog Resources on Addgene.org Learn about the Michael Davidson...Allele Biotechnology that now can be deposited at Addgene. What makes these fluorescent proteins unique and...was then screened through successive rounds of mutagenesis for the brightest teal fluorescent proteins that...scientists from Florida State University, they used a mutagenesis approach and found that specific amino acid changes...scientific community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "Directed evolution...PMCID: PMC3811051. Additional resources on the Addgene blog Read our fluorescent protein blog posts Find...

  3. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...during adeno-associated virus manufacturing." Human gene therapy21.10 (2010): 1251-1257. PMID: 20649475. ...have probably heard about Addgene’s viral service but did you know that Addgene also performs independent...101 eBook Additional resources on Addgene.org Learn about Addgene's viral service Learn about viral production...research? Since launching the viral service in 2016 Addgene’s research team has been actively investigating ...PMCID: PMC2957237. Additional resources of the Addgene blog Read our beginner's guide to viral vectors...

  4. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...Solubility Enhancer. In P. E. Vaillancourt, E. coli Gene Expression Protocols (Vol. 205, pp. 99–118). Humana...-59259-301-1:99 Resources on Addgene.org Addgene antibodies Addgene’s antibody guide Antibody protocols...having solubility issues!). Need a GST antibody? Addgene has you covered! His His tags, often called polyhistidine...Purification of His fusion proteins from E. coli is generally more straightforward and of a higher purity than...and other qualities of each tag class, they are generally best suited for their primary application. Ready...protocols  Resources on the Addgene blog Plasmids 101: Protein tags Antibodies 101: Epitope tags To Each...

  5. What the HEK?

    Type
    Blog Post
    ...affect factors that could skew research results (gene expression, growth rates, etc.). Thus, it’s important...present in this line, making it genetically female. HEKs are generally cultured as an adherent monolayer...plasmids with the SV40 origin of replication. To generate a virus, you need only transfect the ‘helper’ .../fbioe.2021.796991 Additional resources on the Addgene blog Five Popular Model Organisms 10 Tips for...and Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ...

  6. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ...Neddersen characterized a few samples by 16S rRNA gene sequencing which showed the presence of many “dark...Yellowstone National Park (YNP). Extremophiles - phylogenetically diverse microbes living in extreme physical...lab and ThermoCas9 from the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9...particularly useful. Additional resources on the Addgene blog Learn about other Cas proteins Read all of...GeoCas9, another thermostable Cas9 Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

  7. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...by the nearly infinite regulatory capabilities of gene pathways just waiting to be discovered. As we started...Bricking Science.      Additional Resources on the Addgene Blog Learn How You Can Use Video to Share Your... 10 Ways to Share Your Science Resources on Addgene.org Find Protocols and Protocol Videos Check out...

  8. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...at East Tennessee State University. He works with gene expression in Pseudomonas aeruginosa. Follow him...Reading, & Organizing Write for the Addgene Blog Resources on Addgene.org Check out Our Revamped Protocol...laborious, but helped build my understanding of how to generate a manuscript. At the end of the online course,.... Over time that portion of the paper will be generated. After you complete a publication quality figure...figure. Writing a scientific manuscript is like generating a quilt with your lab mates, you add the individual...100percentSDS.     Additional Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific...

  9. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests, though, have...Career Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...than what fits for applied research. I focus in general terms on a couple of very broad topic domains, ...Pam_Hines and LinkedIn. Additional Resources on the Addgene Blog What Do I Do Now? Academic v. Non-Academic...

  10. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ... to examine translation of Drosophila patterning gene oskar. Early in development, the osk-TRICK reporter...Find plasmids from this paper at Addgene. Additional Resources the Addgene Blog Learn More about SunTag... remained elusive. Two techniques developed by Addgene depositors have made it easier to track translation... on the nascent polypeptide recruit scFV-GFP, generating green puncta that colocalize with the red reporter...PMC2746483 Find ribosome profiling plasmids at Addgene. 2. Halstead, James M., et al. “Translation. An...: PMC4451088 Find plasmids from this paper at Addgene. 3. Yan, Xiaowei, et al. “Dynamics of Translation... Brightest Fluorescent Protein Resources on Addgene.org Find Fluorescent Protein Plasmids Check Out ...

  11. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    ... role in the transcriptional regulation of many genes. A number of groups have designed means of studying...with mAID. Using this method, the lab was able to generate conditional alleles of a few essential proteins... al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova et al., Methods, 2019. PubMed PMID:...targeted by the Cas9 nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to recognize...difficult. Find SpCas9 and Target-AID-NG plasmids at Addgene! Nishimasu H et al., Science, 2018. PubMed PMID... MerMAIDs - marine channelrhodopsins for the optogenetic toolbox Article contributed by Susanna Bachle...quickly and with high precision - a valuable optogenetics feature when studying neuronal activity. The...

  12. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...China was using CRISPR/Cas9 in an attempt to create gene-edited babies. Several hours later, the scientist...public consensus on genome editing, there was a generally agreed-upon international moratorium on utilizing...incorrectly known as “three-parent babies,” where fused genetic material from intended parents is “rehoused” in...decisions on scientific, ethical, regulatory, and general public consensus to the next meeting of experts...and the entire 2018 saga of “what’s happening in genetics this time?” She lives with her partner (in business...on theirpenmanship. Additional resources on the Addgene blog Find all of our CRISPR blog posts Download... genome engineering blog posts Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

  13. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...journal club discussion on Kevin Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People kept asking... at Addgene.org Order Plasmids from Addgene! Find Free and Open Educational Resources at Addgene! ...BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge with the community...microbes. We’ve ordered a few yeast plasmids from Addgene and are eager to do our first yeast transformations... @ZebraElement     Additional Resources on the Addgene Blog Experimenting in an Open Source Lab: from...

  14. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...Congratulations, you have a plasmid expressing your gene of interest (YGOI) and are ready to dive into your...received matches the expected sequence. Here at Addgene, we use NGS-based quality control to confirm the...necessary but also an easy process. Please vist Addgene's resource for plasmid verification to find additional...DNA gel.  More Plasmid Eductional Resources: Addgene's Molecular Biology Reference Find More Protocols...

  15. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...protein (FP) tags. Information on genes and associated neurodegenerative diseases was curated from several...but not exhaustive. If you do not see a particular gene of interest, please search our full plasmid collection... Huntington's Disease Parkinson's Disease Others Gene Type Wildtype Mutant Partial Sequence Codon-Optimized... T7 polH GAL Other Clear Filters ID Plasmid Name Gene Tags Promoter Disease PI 1177 p416 25Q GPD HTT GFP... Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a ...Spinal muscular atrophy Eugene Yeo 156073 TARDBP TARDBP V5-MCP EF1a ALS Eugene Yeo 156883 pD649-HAsp-CSF1R-Fc...Parkinson's, FTD Eugene Yeo 214673 lucMAPT-30U MAPT T5, luciferase CMV Parkinson's, FTD Eugene Yeo 214674 lucMAPT-GenRep...

  16. Chemogenetics AAV Preps

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    Collection
    ...Service AAV Chemogenetics Viral Vector Packaging Service: Chemogenetics AAV Use our chemogenetics AAV to chemically...Ready-to-use AAV available from Addgene's viral service encoding chemogenetics tools. Viral...in specific cell types. See our Chemogenetics Plasmids and Chemogenetics Guide pages for more information... Retrograde AAV Inventory Page . Browse all Chemogenetics AAV Don’t See What You’re Looking For? Our Packaged...

  17. Optogenetics AAV Preps

    Type
    Collection
    ...Packaging Service AAV Optogenetics Viral Vector Packaging Service: Optogenetics AAV Optogenetic tools can be used... potentials in neurons. See our Optogenetics Plasmids and Optogenetics Guide page for more information...ready-to-use AAV preparations of optogenetics plasmids available in Addgene's collection. Opsin Excitatory...select plasmids in the repository. Browse our optogenetics AAV collection, including channelrhodopsins,...control cell activity in living tissue with light. Optogenetics is typically used in neuroscience to control...consider using fewer filtering options. Browse all Optogenetics AAV *AAVrg = retrograde serotype, produced with...

  18. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...sequence between two target sites. Modifying multiple genes at once. Using multiplexed gRNAs can target multiple...simultaneous multiplex editing of six different genes. This system doesn’t rely on any additional or host-dependent...from this paper at Addgene. 9. Yin, Linlin, et al. “Multiplex Conditional Mutagenesis Using Transgenic ...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Browse Our CRISPR Topic...add multiple gRNAs on one plasmid. Sound good? Addgene has many tools to help you multiplex - we’ll use...multiplex gRNAs, some being: Using dual nickases to generate a knockout or edit. This can help reduce off-target...Multiplexing gRNAs: the basics One common question Addgene Senior Scientists receive is: can I express more...

  19. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ... distribution via Addgene. For a full list of Addgene's viral vectors See Addgene's Current AAV inventory... from the Penn Vector Core. Chemogenetics (including DREADDs) Optogenetics Recombinases Calcium Sensors... are now available for distribution at Addgene: Penn ID Addgene ID Name Function PI AV-1-49531P 100040...tdtomato Optogenetics Scott Sternson AV-1-20071P 20071-AAV1 pACAGW-ChR2-Venus-AAV Optogenetics Karel Svoboda...mCherry.WPRE.SV40 Optogenetics Karl Deisseroth AV-1-26966P 26966-AAV1 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl...eYFP.WPRE.hGH Optogenetics Karl Deisseroth AV-1-26968P 26968-AAV1 pAAV-Ef1a-DIO ChETA-EYFP Optogenetics Karl Deisseroth...EYFP Optogenetics Karl Deisseroth AV-1-26971P 26971-AAV1 pAAV-CaMKIIa-eNpHR 3.0-EYFP Optogenetics Karl...

  20. Addgene Packaged on Request: Scope of Service

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    Collection
    ...Request: Scope of Service Addgene's Packaged on Request Service is subject to Addgene’s Terms of Purchase and... If Addgene determines that our current queue is too high and we do not expect to meet the general turnaround...with our materials, Addgene cannot guarantee the function of any viral vector. Addgene performs QC measures...produced through our Packaged on Request service, Addgene guarantees a titer of at least 4 x 10 12 GC/mL ... to toxicity or other reasons For these cases, Addgene will promptly notify you by email within a few ...expectations communicated by email will supersede the general guarantees above. We will have our expert viral...not yield the titer guaranteed here or by email, Addgene will contact you to offer some options for moving...
Showing: 681 - 700 of 1141 results