Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 911 results for: IRE

Showing: 681 - 700 of 911 results

  2. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...impacted the experiments we wanted to perform. It required a great deal of time and effort to test and optimize...J. O., Outram, S., Koenig, B. A., Pereira, S., McGuire, A. L., & Lázaro-Muñoz, G. (2020). Researcher Perspectives...

  3. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...availability following cross-linking fixation; they require an additional step generally referred to as Antigen...retrieval here because it’s not quite as ubiquitously required for IF as fixing and permeabilizing.  References...

  4. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...choose, you may not be able to elute the antibodies directly into your preferred buffer. If that’s the case...suggests, you may also concentrate your prep to the desired concentration using these columns. If you are conjugating...

  6. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...the frontiers of fluorescence microscopy often requires more photons. For example, moving from transient...Unlike other systems, no genetically encoded tag is required as the small-molecule binding motif targets endogenous...

  7. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats...integrase-deficient lentiviral vectors for applications that require the large genetic capacity of a lentiviral vector...

  8. CRISPR Antimicrobials

    Type
    Blog Post
    ... PMC4466736. 5. Ando, Hiroki, Sebastien Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular...

  10. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...about its limited resources. Rather than developing entire new teams in house to solve every problem (although...collaborators’ professional developments without directly contacting them. One great way to transition from...

  11. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...solution. Since this technique is intensive and requires an electron microscope, it is not routinely performed...a few ways:  Absolute quantification by qPCR requires generating a standard curve of known concentration...

  12. Lab to Office Culture Shock

    Type
    Blog Post
    ...process of onboarding a new employee who's coming directly from grad school. The transition has been a bit...thought they would be working in a lab for their entire career. Yet even success in academia means you’...

  13. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...drawbacks limit the utility of these systems: St1Cas9 requires a very specific PAM sequence that limits the number...SaCas9, Zhang’s group used an approach called BLESS (direct in situ breaks labeling, enrichment on streptavidin...

  15. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... with a distributor for that country. In China, direct shipments weren’t able to clear import control...., we launched the Addgene blog for topics that required a lot of nuance or detail. By the end of 2023,...

  16. How to Be an Excellent Trainee

    Type
    Blog Post
    ...someone who provides career advice and offers direction on your scientific project. Your scientific mentor...meaningful relationships in your new lab, learn the required techniques, and be ready to take on trainees one...

  17. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...as Rep or other initiator proteins, they do not require host proteins for replication and can maintain ...pneumophila, Methylophilus methylotrophus, Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia...

  18. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...all of these platforms were developing, Google acquired Youtube and has been growing its online video ..., also known as the science videographer, is a retired botanist and oceanographer who has a blog and several...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...best to start from scratch. Obtain a vial of the desired cell line from a reputable distributor such as ...course of infection. These binding events can be impaired by the electrostatic repulsion of the negatively...
Showing: 681 - 700 of 911 results