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We narrowed to 1,106 results for: Ide

Showing: 681 - 700 of 1106 results

  1. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    ...service that we, as scientists, provide for journal editors to help decide whether work is suitable for ...of the 18th century. Yet the peer-review was not widely adopted by the scientific community until the mid...peer-reviewing journal submissions since 2015 for a wide variety of journals. Since then, I developed a routine...submitted paper in addition to the journal-specific guidelines.  Peer-review step 1: The first read At the first...

  2. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ..., Addgene will have unique Research Resource Identifiers (RRIDs) for each antibody, making it easy for...topics are discussed on Addgene’s blog and Antibody Guide, which include information about immunofluorescence...suggestions about how to make the resource better or ideas for new materials to add to the collection, please...find useful antibodies for your research. Please consider sharing your experiences with antibodies from ...Introduction to Immunofluorescence Addgene’s Antibody Guide The project is supported by a NIH BRAIN Initiative...

  3. RUBY-Red Siliques

    Type
    Blog Post
    ...transformation fall into two main categories: antibiotic/herbicide resistance and fluorescent proteins. Selection...undifferentiated plant cell culture), which is great for a wide variety of applications. But some techniques require...CRISPR-Cas9 gene editing, transformation may be identified by resistance to an antibiotic. Successful editing...therefore, are useful for research, and they are identified by a phenotype caused by the segregating mutation...these mutant plants. Using RUBY to simplify the identification of these mutants will speed up my work and ...

  4. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...a new CRISPR screening method that makes use of guide RNAs with internal barcodes (iBARs) in their loop...experimental replicates for each of the gRNAs. The idea was to assign four different barcodes to each of...covers every annotated human gene, it enables genome-wide screening with a high MOI, which is beneficial for... and can serve as a drug-screening platform to identify new antifungals that target calcineurin. Current...

  5. Plasmid Cloning by PCR

    Type
    Blog Post
    ...important to use a high fidelity polymerase to minimize mutations. The fidelity of the polymerase becomes...as Addgene’s Sequence Analyzer, to allow you to identify which restriction sites are present in a given...compatible with a standard PCR reaction (see PCR Video). Therefore, our Forward Primer will use the sequence... chance for mis-priming, so you can use a pretty wide range of annealing temperatures, but you may need...

  6. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...this stage, only cells from the immune system show wide scale mobility with movement from the bone marrow...you can detect signal in thick specimens. See the video below for an example of two-photon intravital microscopy... in diverse tissue environments. These studies provide quantitative measurements of cellular motility,...and skin. Two-photon intravital microscopy. The video above depicts the infiltration of neutrophils expressing...technical specificities have to be taken into consideration especially in the use of fluorescent proteins... Addgene.org Check out Our Fluorescent Protein Guide Pages Find Plasmids for Subcellular Localization...

  7. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...working in academia or industry. At BosLab, we identify ourselves as part of a network of independent ...square, but we saw the potential in the space and decided to reactivate it. We started out by holding a few...from hacking probiotics to filming educational lab videos, while some are looking to develop new skill sets... mind. Thus, to encourage more involvement and provide all our members the opportunity to do molecular...project. This project was inspired by previous city-wide genomic studies and our study will involve getting...

  8. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...product because the plasmids in the library are identical except for the insert; there’s no need to sequence...results represent a successful amplification? To provide a point of reference, Addgene commonly prepares...results If your sequencing is successful, your NGS provider should return results in the form of many, many... want! At the end of the day, it’s up to you to decide your threshold of what a good amplification looks...post has helped clarify some of the important considerations when using, amplifying, and verifying pooled...

  9. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ... the widely used 293T. During vector production, assembled AAV vector particles accumulate inside the ...either associated with the outside surface of the exosome or are carried inside the vesicle, which may also...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

  10. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...smaller fragments that are then barcoded for identification. This mixture of barcoded fragments is known...for each sample remain highly normalized across a wide range of input DNA concentrations. This process ...of 96 plasmids on a plate) will typically have a wide range of input quantities. This variation is problematic...are produced from large numbers of samples with a wide range of input concentrations (via proprietary chemistry...alternative to Sanger sequencing because the sample identity information is always displayed at the individual...

  11. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...scarless DNA constructs using homologous regions to guide the joining reaction. BioBrick Assembly will leave...fragments, and generate constructs for producing single-guide RNAs – but it also allows you to skip purification...NEBuilder HiFi so accurate (apart from the high-fidelity polymerase) is the ability to remove, reset and...freely available online. Its simple interface can guide you through both experimental and primer design ...single-stranded synthetic oligo to generate a single-guide RNA (sgRNA). Using only one single-stranded oligo...

  12. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...Nicholas T., et al. “Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome...sequenced and aligned to the genome to determine genome-wide translational frequency. Although highly useful,...reporter mRNAs. Using translation inhibitors cycloheximide and puromycin, Halstead et al. verified that... translated, the SunTag sites on the nascent polypeptide recruit scFV-GFP, generating green puncta that...

  13. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...situation. But a few days later we had an idea. And this idea rapidly grew and became Crowdfight COVID-...modern science in our view. And it was this very idea that research is done better with others than by...has been essential to push certain projects. The idea behind the platform was simple: any person working...remote collaborations Read our Transferable Skills Guide article about external collaborations Resources...

  14. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...physical location didn’t matter. I didn’t have to consider that aspect and I could plan more with regard ...depending on the conference, the audience can help decide which questions get answered. “You can upvote a...presenting is eye contact. “It’s important to keep your video on and look into the camera to make it seem more...on Twitter where each poster presenter had four “slides” to use for their poster, eliminating the need ...to make space for it,” says Bachle. “On the flip side, you can attend conferences for just a little bit...

  15. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...efficiencies and expression levels in the cells. Ideally, the chosen reporter is unique to the cell, is ... generations. Stably transfected cells may be considered an entirely new cell line from that of the original..., NIH3T3, CHO, 293HEK, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation 100...hygB HeLa, NIH3T3, CHO, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation. ... 10-14 days depending on how quickly your cells divide. Gentamicin is often used as a supplement in mammalian...

  16. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...fall back to? We have outlined some important considerations to help you choose the plasmid prep kit that...or by incorporating recycled paper and plastic. Aside from the more obvious swaps like using smaller bottles...Where can you look for help?  All manufacturers provide specs, protocols, and quality control information...help explain the rationale behind each step and provide troubleshooting advice. In these cases, it can ...between these manufacturers, as price is a large consideration for most labs. Many institutions have preferred...department for these.  While budget is an important consideration, you do want to make sure you’re getting the...

  17. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...Visit Our AAV Guide Page Find Ready-to-use Virus for Your Research Visit Our Lentivirus Guide Page ...understand brain function So far scientists have identified 180 brain regions. Both this geography and the...Using retrograde AAV AAVrg can be used to deliver a wide array of genetic tools for neural interrogation ...where the cell bodies of the projection neurons reside.   Impact for scientists While the mechanisms ...

  18. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...chemical substrate (and sometimes cofactors) to be provided and are generally dimmer than fluorescent proteins... remains popular, other luciferases have been identified in the sea pansy (Renilla reniformis), click ...assays (low background, no photobleaching) but provides flexibility by pairing the luciferase with a variety...change in conformation of a single protein. Considerations for luciferase assays Luciferases can only ...luciferase output at different wavelengths. Addgene provides empty backbones with the luciferase gene, a variety...

  19. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...of dNTP in the reaction mix, the researcher can decide exactly how far the T4 exonuclease proceeds: at...at the first occurrence of the chosen nucleotide, T4 will switch to polymerizing and then stall due to...the “damage” and producing a complete plasmid. Besides being compatible with any vector, the SLIC protocol...multi-fragment DNA assembly, and its low cost makes it ideal for researchers doing large amounts of cloning. ... 10.1007/978-1-61779-564-0_5. PubMed. JBEI-J5’s guide to SLIC Resources at Addgene Ligation-Independent...

  20. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...in the lab. This is commonly done using calcium chloride which permeabilizes the cell membrane so the bacteria...inject their genetic material into the cell. Once inside, phages can follow one of two different life cycles...both of these life cycles bacterial DNA can be accidentally packaged into the newly created phages. Transfer...referred to as transduction. Transferred DNA once inside the infected bacterium can either exist as transient...Addgene’s protocol page, which has both protocols and videos for techniques in basic molecular biology, plasmid...
Showing: 681 - 700 of 1106 results