Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 1,015 results for: LEA

Showing: 681 - 700 of 1015 results

  1. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...subsequently changed fields and my cohort of friends and colleagues had moved on as well, so I knew very few people...people, it was certainly an issue worth addressing. Clear expectations have been established for future participants... after our initial pilot, and look to take some leaps. Binning Singletons is its own organization, working...

  2. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...this gap by both highlighting that researchers do learn MANY transferable skills while working in the lab...to live in your lab, right? Wrong! While it is clearly a good idea to be diligent in your research, remember...career center. Alternatively, it might even be a colleague. A mentor does not necessarily need to be someone...

  3. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...contributed by the Mesothelioma Cancer Alliance. We have learned much about the causes of cancer and the different...nucleus, if the cell dies from injury, this protein leaks out into the cytoplasm, migrates out of the cell...About Cancer and Addgene's Cancer Collection Pages Learn How the ClonTracer Library Can be Used to Study ...

  4. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...for Titering Your Lentivirus Learn All About Viral Vector Elements Learn How to Do Genome-Wide Screens... target cells. Poor tissue culture practices can lead to low titers and poor transductions. Cell lines...

  5. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ..., using up valuable resources in the cell thus leading to slowed growth. Additionally, some protein products...well for most experiments, the lac promoter can “leak,” meaning that a low level of expression exists ...your favorite protein expression tips and tricks! Leave a comment below or browse Addgene’s collection of...

  7. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...very high titers (Zingg et al., 2017), which can lead to cells in far away brain regions expressing your...experiment. Some inducible constructs can exhibit leaky expression in the absence of the inducing drug, ... result in overexpression of your gene and could lead to toxicity or trouble interpreting your results...

  8. Deep Dive: qPCR

    Type
    Blog Post
    ...temperature 5-10 degrees above the primer and be at least 25 bp away from each primer, ideally located close...suitable for your cell lines and/or tissue types.  Please do note my oh-so-subtle use of reference genes,...can be used up quickly by abundant transcripts, leading to falsely lowered levels for less abundant transcripts...

  10. The 12 Days of CRISPR

    Type
    Blog Post
    ...me: Drag-and-drop genome insertion, without DNA cleavage, with CRISPR-directed integrases🎵 On the 6th ...Addgene gave to me: dCas9-based gene editing for cleavage-free genomic knock-in of long sequences🎵On the...

  11. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...this series, we’ll highlight one tip that we’ve learned in the lab that will hopefully make your life a...be able to locate materials quickly and easily. Learn more about using barcodes. Check out our “Lab...

  12. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    ...Guide to Synthetic Biology One of the best ways to learn about a new field is simply to know where to look...have additional resources that might be helpful, please reach out to me by email.  About the author Niko...

  14. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    ...all of the answers, but we’re betting that your colleagues do. That’s why we’re launching the AAV Data Hub...thank you for your contribution to open science.  Please note that the AAV Data Hub is a beta project, and...

  15. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ...’ll discuss the types of programs SITN runs and learn how they think about communicating science. Listen... our Interview with Science Rapper Tom McFadden Learn about SITN's work at DayCon 2015 5 Reasons to Use...

  16. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...really incredible science - I hope you've enjoyed learning about it (and maybe even picked up a new technique...a research story you want to share on our blog, please reach out to [email protected] - we'd love to hear...

  17. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...cleverness, and crafting skills have surpassed your colleagues. Success is sweet – as sweet as all that Halloween...yourself on in the coming days. Hopefully, I’ve made clear how excited we get for Halloween and how seriously...

  18. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    ...shared might have you wondering how many plasmids leave Addgene headquarters every day. That would be an...celebration was so much fun! Here's the Addgene Leadership team in all their photo booth glory! #1millionplasmids...

  19. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...Founders - Melina Fan, Ken Fan, and Benjie Chen. We learn what it was like to get Addgene off the ground and...idea got off the ground Chapter 3 - Addgene as a leader in standardized tech transfer and the adoption ...

  20. AAV Packaged on Request is Here!

    Type
    Blog Post
    ... (AAVrg), and volumes of 1 mL, 2.5 mL, or 5 mL. Please note that all orders will be shipped in 100 µL ...Request webpage, or our announcement blog post to learn more. And our helpful scientific support team is...
Showing: 681 - 700 of 1015 results