..., using up valuable resources in the cell thus leading to slowed growth. Additionally, some protein products...well for most experiments, the lac promoter can “leak,” meaning that a low level of expression exists ...your favorite protein expression tips and tricks! Leave a comment below or browse Addgene’s collection of...

...a first time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an ..., would help improve split vector strategies and lead to greater and more consistent transgene expression...

...very high titers (Zingg et al., 2017), which can lead to cells in far away brain regions expressing your...experiment. Some inducible constructs can exhibit leaky expression in the absence of the inducing drug, ... result in overexpression of your gene and could lead to toxicity or trouble interpreting your results...

...temperature 5-10 degrees above the primer and be at least 25 bp away from each primer, ideally located close...suitable for your cell lines and/or tissue types.  Please do note my oh-so-subtle use of reference genes,...can be used up quickly by abundant transcripts, leading to falsely lowered levels for less abundant transcripts...

...episode of our Hot Plasmids podcast series, you'll learn about optogenetics tools for controlling protein...plasmids covered in this segment Hot Plasmid 1: Photocleaveable PhoCl protein for controlling molecular processes...

...me: Drag-and-drop genome insertion, without DNA cleavage, with CRISPR-directed integrases🎵 On the 6th ...Addgene gave to me: dCas9-based gene editing for cleavage-free genomic knock-in of long sequences🎵On the...

...Guide to Synthetic Biology One of the best ways to learn about a new field is simply to know where to look...have additional resources that might be helpful, please reach out to me by email.  About the author Niko...

...If you have any questions on ordering plasmids, please feel free to email [email protected]. We’ll be happy...regulations are complex and may change suddenly. Please email [email protected] for the most-up-to-date ...

...this series, we’ll highlight one tip that we’ve learned in the lab that will hopefully make your life a...be able to locate materials quickly and easily. Learn more about using barcodes. Check out our “Lab...

...all of the answers, but we’re betting that your colleagues do. That’s why we’re launching the AAV Data Hub...thank you for your contribution to open science.  Please note that the AAV Data Hub is a beta project, and...

...’ll discuss the types of programs SITN runs and learn how they think about communicating science. Listen... our Interview with Science Rapper Tom McFadden Learn about SITN's work at DayCon 2015 5 Reasons to Use...

...really incredible science - I hope you've enjoyed learning about it (and maybe even picked up a new technique...a research story you want to share on our blog, please reach out to [email protected] - we'd love to hear...

...cleverness, and crafting skills have surpassed your colleagues. Success is sweet – as sweet as all that Halloween...yourself on in the coming days. Hopefully, I’ve made clear how excited we get for Halloween and how seriously...

... (AAVrg), and volumes of 1 mL, 2.5 mL, or 5 mL. Please note that all orders will be shipped in 100 µL ...Request webpage, or our announcement blog post to learn more. And our helpful scientific support team is...

...shared might have you wondering how many plasmids leave Addgene headquarters every day. That would be an...celebration was so much fun! Here's the Addgene Leadership team in all their photo booth glory! #1millionplasmids...

...Facebook Additional Resources on the Addgene Blog Learn About Our Recent Visit to China Read Zafira Castano...Recent Post on the International Mentorship Program Learn How to Network and Meet People at Scientific Conferences...

...Founders - Melina Fan, Ken Fan, and Benjie Chen. We learn what it was like to get Addgene off the ground and...idea got off the ground Chapter 3 - Addgene as a leader in standardized tech transfer and the adoption ...

...happy during their time in grad school. As you'll learn, grad school has its ups and downs for everyone ...Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve the ...

...changed its name from Novophage to Sample 6. To learn more about Novophage's successful transformation...Our Podcast Series Get Additional Startup Advice Learn How to Be A Good Manager from Addgene Executive ...

... we stick to citation counts for a moment, it’s clear that articles in less-prestigious journals have ...citations to all articles, Anurag Acharya and colleagues report, the percentage of citations to articles...