We narrowed to 928 results for: Mpl;

...gene of interest is cloned into/inactivates or completely removes (in the case of Gateway cloning) one ...Posts Learn How to Use Blue White Screening to Simplify Your Cloning Experiments See Where You Can Find...

...reporter. Quantification of editing efficiencies simply involves tracking the ratio of mCherry-positive...APOBEC3A-Cas9n-UGI and APOBEC3B-Cas9n-UGI base editing complexes are more efficient than the BE3 plasmid containing...

...efforts is the Golden Stopwatch Award to celebrate exemplary TTOs. Our new Golden Stopwatch Award recognizes...agreements slow down your research; provide specific examples if possible Tell them about the Golden Stopwatch...

...educational materials to our repository of open samples. We are, and have always been, a community resource....43322 Bandrowski, AE, Martone, ME (2016) RRIDs: A Simple Step toward Improving Reproducibility through Rigor...

...cannot be indirectly probed simultaneously on the sample. What to do?  If you’ve ever found yourself facing...visualize both targets in parallel (Fig. 2). For example, if both of your antibodies are rat IgG1, then ...

...Pharmaceutical in 2017. As one of the original employees at Dimension Therapeutics, she helped build and...manufacturing and quality, especially with regard to compliance with health authority regulations,” Baradaran...

..., this cell free system allows for a relatively simple, controlled “one-pot” reaction capable of reproducing...found in the metagenome datasets from hypersaline samples, were engineered and adapted for RNAi experiments...

...able to mediate homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication...(so called “gDNAs”) rather than the RNA guides employed by Cas9 or Cpf1. These short, 24 base DNA guides...

..., in turn, the big picture of the project. For example, how can future regulations impact your work? What...sounds interesting, TAKE ACTION! Taking action: Examples of extracurricular activities The table below...

...breaks down the HMO of interest into its component simple sugars (including lactose), and a lac operon controlled...to determine the amount of HMO originally in the sample. The second sensor is a fusion protein consisting...

...Thus, the bEFPs bind the UAS via the dCas9-gRNA complex which allows transcription from the promoter.  ...should serve as both a powerful tool for generating complex regulatory systems and further the study of natural...

...binds to TIR1 of the SCF-TIR1 ubiquitin ligase complex, promoting its interaction with the degron sequence...used ChRs in that they cause a rapid and almost complete desensitization when exposed to continuous bright...

...size 20 µL samples of control AAV that scientists can use for serotype testing. These samples are available...

...other ways to make your session more engaging, for example, by using short animations and visuals, or asking...be a “place” for networking within the app. For example, the NeuroSymposium had virtual tables that you...

... binding affinity to major histocompatibility complexes (MHC class I and II).  There are multiple algorithms...approach The in silico approach cannot replicate the complexity of antigen processing events that take place ...

...from a month to a year. The researcher remains employed by their home institute, meaning the host university...and a priority list of experiments you hope to complete. Share this plan with your host prior to arrival...

...JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587...antibodies allows you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal...

... proteins should be separated by 50-60 nm. For example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm...protein of interest has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer...

...an interview setting, this means persuading an employer that your past work has prepared you to contribute...audience and ask for their feedback before you complete your work. If you’re giving a public talk, ask...

...interact with each other. Chromatin regulation is complex and influences--directly or indirectly--many other...phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling...