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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 910 results for: Nes

Showing: 681 - 700 of 910 results

  1. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...can be identified. Even if some mammalian cells lines have similar morphologies, being familiar with these...Twice a week often works for most fast-growing cell lines (such as HEK293, which multiplies every 16 hours...

  2. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...proteins both transiently or through stable cell lines. Both methods produce high protein yields if transfection...cells)  that allow for continuous expression of genes integrated into the insect cell genome. Both of ...

  3. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...that could directly impact patients. And, to be honest, I’ve never enjoyed grant-writing. The more I talked...academia, however, writing papers and grants is an inescapable and essential part of being able to continue ...

  4. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...SpCas9 (derived from the bacterium Streptococcus pyogenes). Consequently, fewer genetic tools for targeted...nuclease-competent Cas9, obviating the need to generate new cell lines or transgenic animals expressing dCas9 as a means...

  5. Pooled Library Amplifications

    Type
    Blog Post
    ...other colonies containing smaller, recombined backbones that may replicate faster. For this reason, if...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

  6. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...CRISPR plasmids categorized by year of deposit. Outlines show a representative proportion of CRISPR plasmids...of tools seen in Figure 3, indicates that the usefulness of popular CRISPR tools is both enduring and ...

  7. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ... the hierarchy of web pages the plasmid page is nested in. You can see in the example above that the bread...Database has more information about common vector backbones.  The backbone modifications listed can refer ...

  8. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...protocols and examples of applications from across disciplines now available. In this post, we’ll review the...example, cytoskeletal structures that form continuous lines in pre-expanded samples can sometimes take on a ...

  10. MXS Chaining

    Type
    Blog Post
    ...derived from the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin ... might not, however, be appropriate for cloning genes from organisms with high CpG dinucleotide representation...

  12. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...variety of chromogenic, chemifluorescent, and chemiluminescent substrates are available. Labs with limited...reader as opposed to chemifluorescent, and chemiluminescent methods that require specialized equipment...

  14. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ... than optimal user experience and the overall soundness of the software design. Each of us brings slightly...Demo details for this would be something along the lines of showing that the checkout takes the user directly...

  15. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...easier. You should also demonstrate a strong willingness to help others with their problems, a general...sorts of details about the application, just not the ones that matter. Therefore, if there is a bit of a Sherlock...

  16. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...Other fields, often highly technical or skills-based ones, may not use them very often. Similarly, entry-level... cover letter should be no more than a page. Conciseness is key! If possible, address to the professor...

  17. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...base pairs long and found upstream of their target genes. The sequence of the promoter region controls the...elements, insulators, and silencers; however, some "leakiness" of transcription may occur. This is normally ...

  18. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...talks for non-academic settings. Many of these guidelines will apply to any type of science presentation...supposed to be done. They will start looking at their phones. Speaking longer just makes you seem unprepared...

  19. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD to manipulate...illumination, substantial PA accumulates in the plasma membranes, indicated by the PA probe colocalizing with SuperPLDx30...
Showing: 681 - 700 of 910 results