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  2. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...with the previously established physical map. In comparing pairs of targets ~2 Mbp apart, they noticed that...that they could evaluate the degree of chromatin compaction even for this small distance! To the authors’...

  3. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ... next steps and follow up especially in large companies. It is fine to address the cover letter “To Whom...If the organization is small, any person at the company might be able to help you. If the organization ...

  4. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    .... “Initial and sustained motivation is a huge component of success.” 2. “Enjoy Teamwork.” Be friendly...friendly, open, sharing, rather than intensely competitive. Be curious and interdisciplinary.   3. “There are...

  6. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...an ‘-omics’ study or is resistant to specific compounds, links to publication references, and cross-references... publications cited. If the information seems incomplete or unclear, Bairoch follow up via email to the...

  9. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...optimal growth temperature of 55 °C. For a brief comparison of GeoCas9 vs. SpCas9, see the table below and...Yes Longevity in Serum?* No Yes Table 1. Comparing SpCas9 and GeoCas9. *For in vitro Cas9 RNP activity...

  10. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...started running on a treadmill. After being with the company for about six months, I learned there was a Genzyme-based...visit us and watch fellow Run4Rare team members complete the Boston Marathon, he wanted to run and play...

  11. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...or leading. All three roles are important to accomplishing any goal or finishing any project. Doers are...Leadership Insights for Engineers, Scientists and Computer Professionals as Leaders, by Ken Graham, Ph.D....

  12. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...training from an organization such as COMPASS. Although COMPASS has a special interest in ocean science...

  13. Advice for Choosing a Research Project

    Type
    Blog Post
    ...typical research project can take several years to complete and has multiple benchmarks. Think about what ...Researchers should  pick a topic that “looks at the complexity of biology and break apart a piece that can address...

  14. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...protospacer adjacent motif (PAM). Once the CRISPR/Cas complex binds to the foreign DNA, a cut is made to destroy...eight evolutionarily conserved CRISPR subtypes comprised of more than 40 cas gene families have been characterized...

  15. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...targets.  Pre-formed primary and secondary antibody complexes (Figure 2B) Some labs have found that you can ...). One issue with this strategy is that large complexes may not permeate through your tissue samples as...

  16. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...CellTagging. By constructing lineage trees and comparing them with the gene expression changes of more ...CellTags for tagging two cell populations in a competitive transplant assay in the mouse intestine, demonstrating...

  17. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and cell ...several pooled libraries: Cas13X and Cas13Y, two compact Cas13 proteins found in the metagenome datasets...

  19. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ...secondary structure and high GC content, but some companies now offer Sanger sequencing conditions which can...
Showing: 681 - 700 of 931 results