We narrowed to 1,017 results for: PLE;

...tens of thousands of biomolecules each containing multiple binding crevices that can nonspecifically soak...measureable FRET will be swamped out by a sea of uncoupled donors. The best method for targeting a fluorescent...NTA binds to the His tag on your protein and the coupled Cy fluorophore acts as the FRET acceptor. Binding... calibrated FRET distance measurements (for an example see Mahalingam et al., 2014).  Many thanks to...

...lab? Addgene is after all a place that encourages people to take some time out every once in a while – just... stress in adolescents. It also put those young people in a better mood. Laughter itself can sometimes...and, of course, it’s a way to connect with other people. (We don't need evidence for that one, do we?) ...

...export controls and regulations are complex and may change suddenly. Please email [email protected] for the ...If you have any questions on ordering plasmids, please feel free to email [email protected]. We’ll be happy...

...Watson & Crick - Science History Rap Battle A great example of science story telling following the unfortunate...importance in understanding biological history. 4. Please Don't Kill My Hive Beautifully filmed video about...science educator looking for creative ways to implement the next generation science standards and communicate...

...sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide multiple streams for composting... new programs, we're always striving to set an example of environmental stewardship.  Additional resources...

...single base changes in the DNA sequence without completely cutting the DNA. RNA Editors: CRISPR nucleases...CRISPR nucleases that can’t cut DNA (dCas9, for example) that are used to increase or decrease expression...library of gRNAs with a CRISPR nuclease target multiple genes in a cell population. The resulting mutant...

...pharmaceutical company based in Switzerland. After completing her PhD in Chemistry and Chemical Engineering...working in industry and how delegating can empower people Chapter 3: How Crystal advanced at Novartis, and... and the importance of realizing that different people have different management needs. How Crystal developed...

...gene/target, name, application, pass/fail rating, sample species, antibody species, or whether the results... by Addgene's curation team for accuracy and completeness. The information is then formatted and published...application. The submission process is easy, with simple, descriptive fields, drop-down boxes when appropriate...

... they have developed this further to provide multiplexed detection, higher sensitivity, and more. Read...lab compared the efficiency and specificity of multiple wild-type and engineered variants of Cas nucleases...included in the next What’s New in CRISPR blog post, please let us know! Additional resources on the Addgene...

...looking to delve deeper or stay tuned, there are plenty of online resources available. I recommend a few...Science Translational Medicine have made their complete collection of relevant papers freely available...you have information or another resource to add, please tell us about it in the comments. ...

... Aavery in this Zoom video background. Want the purple bacteria video background with mascots Blugene ...and more!) on Addgene’s Help Center. Download the purple bacteria background.       Are none of those ...Addgene’s Science Careers blog topic. Download the purple lab-themed background.     We hope these science...

...template covalently attached to the Cas9-gRNA RNP complex via the fused HUH endonuclease PCV. This increases...Neisseria meningitidis, Nme2Cas9, that recognizes a simple dinucleotide PAM (N4CC), lending to high target...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

...results Above (top right corner of this post) is an example of a seemingly clean DNA sequence (no Ns in sight...less and you might suspect contamination in your sample or consider asking your sequencing facility to ...Use a silica spin column for purification of the samples you send for DNA sequencing If your sequencing ...

... need a high efficiency of transformation (for example, if you are working with a library or an especially...making competent cells are available online. A few examples are provided here for reference: Untergasser ...transformation. This protocol has worked well for us for multiple E. coli strains, including DH5alpha, XL1 Blue,...

... of the coast of Washington state. Using these samples, he was able to isolate two proteins from the jellyfish...potential as a research tool. Soon, the number of people studying it grew. In particular, studies in the...organisms to find the culprits. He even obtained samples of certain ones from local pet shop aquariums in...

...Stefan Stricker’s lab, is a single step gRNA multiplexing system that generates vectors containing high...efficiency can be controlled by manipulating complementarity between the gRNA and target. Read the paper...included in the next What’s New in CRISPR blog post, please let us know! Additional resources on the Addgene...

...scientific community? Access to plasmids which gives people more time to focus on their research.  How will...plan for moving forward. Sooner or later enough people will be vaccinated and we’ll have some herd immunity...raises questions like “How do you start to bring people back to on-site work?” and “How do you start to...

...cassettes for selection of transgenic plants. The complete GreenGate toolkit, now available at Addgene, also...tested GreenGate in Arabidopsis, it should in principle work in any plant species. Tricks of the trade...essential for scientific progress. Also, the more people use such a system, the more building blocks will...

...interviewing fellow Addgenies. I spoke with amazing people that were a part of the different departments of...felt like I could actually make connections with people. These were some of the things that really made...by Addgene. I have met some of the most genuine people who have shared amazing advice with me. Due to ...

...Science Communication group started. It's as simple as getting people together at a bar. Chapter 10: Maintaining...well as a platform for scientists to be viewed as people (#ActualLivingScientists). We also discuss the ...