Which Fluorescent Protein Should I Use?
Type
Blog Post
...effect called FRET (fluorescence [or Förster] resonance energy transfer). FRET occurs when energy transfer...
We narrowed to 739 results for: SON
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Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...Medical Center. You’ve probably heard of Forster Resonance Energy Transfer (FRET). Through the non-radiative... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...off-target editing. In one direct transcriptome-wide comparison, REPAIRv2 reduced off-target sites from the 18,385... -
AAVs in Retinal Gene Therapy
TypeBlog Post...gene therapy revolution and the development of personalized medicine. References and further reading 1.... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post...finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator where... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post...lines in B-D represent the pure DNA spectra for comparison. Each of these contaminants will have a slightly... -
Getting to Know Expansion Microscopy
TypeBlog Post.../10.1126/science.1260088 Damstra HG, Mohar B, Eddison M, et al (2022) Visualizing cellular and tissue... -
Great Results Start with Great Standard Curves
TypeBlog Post... ELISAs and When to Use Them A Control for All Seasons More resources on addgene.org Addgene's Antibody... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...single flask. Through a bit of stochastic magic and Poisson statistics, each sub-population has, on average... -
Plasmids 101: Colony PCR
TypeBlog Post...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org ... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...43:729-736. PubMed PMID: 3907859. 4. Fields, S., Song, O. A novel genetic system to detect protein-protein... -
Plasmids 101: Protein tags
TypeBlog Post... Additional resources: Young CL, Britton ZT, Robinson AS, Biotechnology Journal 2012 , (7): 620-634.... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post...boring. I think that such tendency is one of the reasons why we have only three biology-related Nobel prizes... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post... among the earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study ... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post... 1. Atasoy, D., Y. Aponte, H. H. Su, and S. M. Sternson. 2008. “A FLEX Switch Targets Channelrhodopsin... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...the ability to cleave the RNA target. Table 1: Comparison on Common CRISPR Enzymes Name Enzymatic Domains... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post...Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base editing of ... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post... and updated April 16, 2020. Figure 1: Comparison of SHERLOCK and DETECTR nucleic acid detection... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...plasmid. Asymmetric plasmid loss occurs for several reasons, including one plasmid blocking replication of ... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post...computed during generation of the ICE Score, the Pearson correlation coefficient (r) is also computed and...