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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,045 results for: STI

Showing: 681 - 700 of 1045 results

  1. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...Oliver Griesbeck of the Max Planck Institute for Neurobiology has been working on genetically encoded...these Twitch sensor variants were optimized by testing various linker configurations in a large-scale ... is disrupted in the presence of a ligand (they stick together in one state, but not the other). “It’s...

  2. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...for making your meetings productive: Discuss logistics if appropriate:  How often will you meet? How ...smaller, actionable steps (e.g. Meet 10 PhDs with interesting careers, find a summer internship, etc.). It ...Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring"    ...

  3. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ... Which graduate student hasn’t been asked the question: “Academia or industry?” Once academia was the...opportunities beyond academia. Once a profession sounds interesting, TAKE ACTION! Taking action: Examples of extracurricular...'d like to learn more about next. Leave your suggestions in the comments section.  ...

  4. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...ug/ml pure dsDNA. Use the following formula to estimate your DNA: Concentration (ug/ml) = A260 reading...sensitivity at low concentrations of DNA and it can’t distinguish between DNA and RNA. See our protocol about using...dyes that fluoresce when bound to DNA. The main distinction here is that these dyes, such as PicoGreen or...

  5. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...human proteins that can be targeted (hopefully by existing therapeutics that are safe, effective, and already...ways by the pandemic, it is clear that we have to stick together (6 feet apart), to fight the new Coronavirus... all people, not just scientists! *If you are requesting plasmids for coronavirus or COVID-19 research...

  6. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...via restriction enzyme digestion, and used directly in DNA assembly will still yield scarless assemblies... well-designed fragment overlap sections, while still creating scarless plasmid inserts.  Want to Learn...

  7. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...avenues previously unavailable to them. With these fantastic tools it’s now easy to monitor protein expression...loops on either side of an enzyme’s active site and still block enzyme function upon exposure to ultraviolet...sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter...

  8. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ... by an anterograde AAV was shown to drop off drastically with lower titers. As you can see in figure 2...many more viral particles to your solution while still using a volume that’s compatible with your experimental...fewer opportunities your viral particles have to stick to your tubes, the higher titer you will have. When...

  9. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...companies and BosLab has grown into an organization consisting of over twenty members and volunteers. Transforming... prior to getting started. For example, Timothy Stiles joined BosLab last year with a computer science...developed community projects. Recently, Timothy Stiles and Mrina Nikrad have started the Open Yeast Engineering...

  10. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...enough to engineer it to suit our purposes? These questions can be addressed using R bodies: simple, force-generating... modified to extend at different pH levels. Domesticating R bodies We have shown that all three of these...extends to rip the bacteria apart; however, they still remain attached to the ciliate. It’s thought that...

  11. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...understands that no question is “dumb” and that it is better to ask before wasting time. Focus on results...support. The manager must be available to answer questions. If you are perceived as too busy to be approachable...

  12. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ... not naturally exist in mammals, scientists can still reap the benefits of plasmid-based research using...that you may not want the plasmid depleted, but still want to use transient transfection methods. Since...* Mode of Action** Working Concentation*** Blasticidin bsd  HeLa, NIH3T3, CHO, COS-1, 293HEK Inhibits...

  13. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Anterograde and retrograde tracing take advantage of existing transport pathways in neurons. Anterograde transport...neuroscientists for some time due to their advantageous characteristics for brain research and their ability to introduce...Addgene). The Gradinaru Lab at the California Institute of Technology generated novel AAVs (Chan et al...Samaranch, L., Kells, A.P., Mittermeyer, G., San Sebastian, W., Zhou, S., Beyer, J., Forsayeth, J., and Bankiewicz...

  14. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...control AAV in smaller aliquots of 20ul for serotype testing to help you pick the best AAV for your experiment...GABA-ergic interneurons (83895). See our Serotype Testing AAV inventory for the entire collection! Plasmid...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...

  15. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...and its potential applications. Challenges of existing mutagenesis methods Several continuous diversification...be used to map protein-protein interactions, investigate non-coding segments of the genome, or engineer...flexibility to address many different types of research questions. Are you ready to start EvolvR-ing your targeted...

  17. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ... at the Hotchkiss Brain Institute. He previously worked at The Allen Institute for Neural Dynamics and...multiple brain regions or cell types at once using existing indicators. For example, you might want to use...

  18. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...traditional restriction enzyme cloning uses short sticky ends to insert genes into plasmid backbones, base...generated in several ways. One option, imprecise T4 digestion (no dNTPs needed), allows both insert and vector... are generated in the linearized vector by T4 digestion; then the components are combined and annealed...

  19. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...promoters, origins, tags, and certain ORFs. Their sophisticated detection algorithm easily and precisely identifies...Sequence” tabs. Primers This tab contains a table listing commonly used primers detected in a given nucleotide...thanks to you (our community of scientists) for suggesting these changes, and the SnapGene and Addgene software...

  20. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...an important feature of a fluorophore. The key question is how the brightness is best defined or determined... cells within the dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct...precise determination of brightness in cells, under realistic experimental conditions. By determining the brightness...
Showing: 681 - 700 of 1045 results