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  1. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...initial assumption. Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts...integration activity in human cells. Moreover, they optimized the specific attachment sites via sequence engineering...

  2. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...regulated to ensure that it occurs at the appropriate time—for example, the cell must have enough nutrients... its job — although ATP does get hydrolyzed over time, inactivating the protein binding it (Grimwade et...

  4. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...coli are easily propagated and have a generation time of around 20 minutes, they are perfect for molecular... Rat physiology is also easier to study and at times more closely corresponds with human diseases and...

  5. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...State-of-the-art FPs (listed in Table 1) were optimized for efficient binding of endogenous BV.  When ...intensities in NIR. Usually, mercury lamps are suboptimal, metal halide lamps are better, and xenon lamps...

  7. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...enhance targeting to the nucleus, and several codon optimized Cas9 variants have been created in an effort to...correct CRISPR components for your application, it is time to deliver these components to your target cells...

  9. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present...present on different cell types and sometimes vary between target species or even strains. Pro tip! Getting...

  10. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...important that the digest go until completion. The time required for complete digestion varies for different...speeds; after running the gel for some duration of time, these differently sized pieces will be in different...

  11. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...concentrate your lentiviral particles to get them to the optimum concentration to infect your cells of interest....References 1. Miller, A. DUSTY, and C. A. R. O. L. Buttimore. "Redesign of retrovirus packaging cell lines ...

  12. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...and vice versa? We draw a parallel between the timelines of the scientific advancements and the noteworthy...amino acid recognition sites had to be designed each time we wanted to target new genomic sites. The discovery...

  13. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...Class I TEs are considered replicative since every time they jump they make a copy of themselves. This increases...active in mammalian cells compared to the codon-optimized wild-type piggyBac transposase.  piggyBac plasmids...

  14. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...are the most efficient (i.e., happen most of the time). Figure 1 shows a graph from the BD® Spectrum Viewer...show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of about...

  15. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...This is not an extensive list Acceptor labeling: Sometimes it's okay to be wrong In contrast to FRET donors...PubMed PMID: 16908183. 10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A...

  17. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...new sections, including our popular "Dimers and Multimers" post. It's also been re-organized and updated...

  19. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    ...techniques — but perhaps are a little limited on time — check out Addgene’s new Quick Tip series on YouTube...
Showing: 681 - 700 of 925 results