We narrowed to 931 results for: TIM

...notebooks in the ELN, without having to add at the time of deposit, makes it far more likely that deposits...commercialization community in greater Boston, while at the same time keeping one foot on the other side of the pond through...

... pKABE7.9, and pKABE7.10) so that the user can optimize base editing efficiency based on the location ...editors, keep in mind that the generation of indel sometimes may also results in RE site destruction. Many...

...regulated to ensure that it occurs at the appropriate time—for example, the cell must have enough nutrients... its job — although ATP does get hydrolyzed over time, inactivating the protein binding it (Grimwade et...

...State-of-the-art FPs (listed in Table 1) were optimized for efficient binding of endogenous BV.  When ...intensities in NIR. Usually, mercury lamps are suboptimal, metal halide lamps are better, and xenon lamps...

...coli are easily propagated and have a generation time of around 20 minutes, they are perfect for molecular... Rat physiology is also easier to study and at times more closely corresponds with human diseases and...

...again. So useful, yet so finicky to design and optimize. Today we’ll cover the normalization and loading... Design of a Western Blot Troubleshooting and Optimizing a Western Blot Resources on Addgene.org Addgene's...

...such as the brightness, photostability, maturation time, and acid sensitivity of various fluorescent proteins...References Adjobo-Hermans, Merel JW, et al. "Real-time visualization of heterotrimeric G protein Gq activation...

...enhance targeting to the nucleus, and several codon optimized Cas9 variants have been created in an effort to...correct CRISPR components for your application, it is time to deliver these components to your target cells...

...signals. Shading denotes SEM. D) Summary of ΔF/F0, Time-to-peak, Rising τ, and Decay τ of the three sensors... a snapshot of interacting proteins at a certain time and genomic location. TurboID improved on other ...

...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present...present on different cell types and sometimes vary between target species or even strains. Pro tip! Getting...

...desired chimeric mice, you would breed them many times, hoping to produce a mouse with the desired edit...template with 55-100 bp homology on each side. For optimal repair, place the loxP sites precisely at the cut...

...important that the digest go until completion. The time required for complete digestion varies for different...speeds; after running the gel for some duration of time, these differently sized pieces will be in different...

...Class I TEs are considered replicative since every time they jump they make a copy of themselves. This increases...active in mammalian cells compared to the codon-optimized wild-type piggyBac transposase.  piggyBac plasmids...

...and vice versa? We draw a parallel between the timelines of the scientific advancements and the noteworthy...amino acid recognition sites had to be designed each time we wanted to target new genomic sites. The discovery...

...concentrate your lentiviral particles to get them to the optimum concentration to infect your cells of interest....References 1. Miller, A. DUSTY, and C. A. R. O. L. Buttimore. "Redesign of retrovirus packaging cell lines ...

...This is not an extensive list Acceptor labeling: Sometimes it's okay to be wrong In contrast to FRET donors...PubMed PMID: 16908183. 10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A...

...are the most efficient (i.e., happen most of the time). Figure 1 shows a graph from the BD® Spectrum Viewer...show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of about...

... a Western blot can be a bit daunting for first-timers. That's why we gave our written protocol a video...

...new sections, including our popular "Dimers and Multimers" post. It's also been re-organized and updated...

...techniques — but perhaps are a little limited on time — check out Addgene’s new Quick Tip series on YouTube...