...capsid variants could unintentionally limit the utility of candidate capsids.  Are you interested in using...

...laundry list of molecules to screen has unclear utility. You cannot predict what will happen. Without the...

...t have to be long and it doesn’t have to be revolutionary, but you could include a few comments on where...

...Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything...

...PILATeS suitable for live imaging with a time resolution of minutes.  Find PILATeS plasmids here! Zhong...

... AAVs preps from 2016–2023. B) Inset showing distribution of only GCaMP6, jGCaMP7, and jGCaMP8 per year...

...interacts with DnaA, stabilizing its binding and contributing to DNA unwinding. This promotes initiation at...

...compatible or not.  Using plasmid incompatibility for therapeutic advantage Although it may seem like plasmid incompatibility...

...and its subsequent repair. Additionally, the distribution of enrichment and depletion scores for safe-...

...experiments. There are far too many behaviors that have utilized optogenetics to be fully summarized in a short...

...? As mentioned above, many expression plasmids utilize inducible promoters, which are 'inactive' until...

...ensure that you are in compliance with your institution’s biosafety regulations. Last Update: February...Leupeptin, Millipore Sigma L2884 Aprotinin saline solution, Millipore Sigma A6279 Before Starting Warm the...µm filter. Prepare 10 mL aliquots and store the solution at -20 °C. 1 mg/mL PEI-MAX Add 1 g of PEI-MAX ...but check for the presence of particles still in solution. Continue to stir until all particles have dissolved... to ensure that it has not drifted. Filter the solution through a 0.22 µm filter. Prepare aliquots and...at 4 °C until use. We suggest preparing fresh solutions after one month. BCD Feed 500 mL BalanCD HEK293...at 4 °C until use. We suggest preparing fresh solutions after one month. 1000X protease inhibitor cocktail...

...considered when interpreting results.  The recent revolution in genome editing methods (most notably CRISPR...

...strand. Each degenerate primer set contains three consecutive randomized bases (NNN) at a given codon site ...

...engineering world by storm owing to its ease of use and utility in a wide variety of organisms.  While much of ...

...Addgene to accelerate biomedical research by distributing antibodies developed by IPI. Our integrin antibodies...

...local temperature sensing with sub-millisecond resolution and sub-degree temperature differences under ...

...protocol, multiplex IF can give you impactful and beautiful results. Performing an immunofluorescence experiment...

...must contact your institution’s Bio-Safety office to receive permission and institution-specific instructions...sulfate for polybrene. c. Add lentiviral particle solution from step E. For a 6 cm target plate, add between...Please follow all safety guidelines from your institution and from the CDC and NIH for work in a BL2 facility...safety practice to follow, please contact your institution’s safety office. For more information, please ...extract 10g sodium chloride 15g agar Prepare LB agar solution by dissolving 40g of LB powder in 1L of distilled...Hexadimethrine Bromide (Polybrene) Prepare a 1mg/mL solution of polybrene (Sigma-Aldrich catalog #H9268) in...in 0.9% NaCl. Autoclave to sterilize. Stock solution is stable at 4°C for up to one year. The powder form...

...cuvette. Pulse. Caution Electroporation involves the use of high voltages, please use caution when activating... a second and then third 1:100 dilution). Plate 100 µL of each dilution onto a prewarmed Petri dish. Incubate...adequate time to fully gel and to dry slightly. We routinely use 350 mL of LB Agar per bioassay plate. 14 mL... mix the four tubes. Perform sequential 1:100 dilutions of the cells (add 10 µL of the pool to 990 µL ... colonies can be too great to count. Ideally, dilutions would have been sufficient to enumerate single...plate, count the second plate (ie. the 1:10,000 dilution). After 12-18 hours of growth, use spreader and...suspended in cryoprotectant like Glycerol or DMSO solutions. Quantify the individual Maxipreps by Nanodrop...